CYPSD - Bacreria201-250

Reference

Cytochrome P450 HomepageNelson, DR (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.AlphafoldJohn Jumper, Richard Evans, Alexander Pritzel, et al. - Highly accurate protein structure prediction with AlphaFold. Nature, 1–11, doi: 10.1038/s41586-021-03819-2 (2021)ColabfoldMirdita M, Ovchinnikov S and Steinegger M. ColabFold - Making protein folding accessible to all. bioRxiv, doi: 10.1101/2021.08.15.456425 (2021)Localcolabfoldhttps://github.com/YoshitakaMo/localcolabfold

Cytochrome P450 HomepageComprehensive Bacterial P450shttps://drnelson.uthsc.edu/comprehensive-bacterial-p450s/

D. R. Nelson's

Bacteria_1

Bacteria_2

Bacteria_3

https://twitter.com/P450nelson/status/1501396401317883904

Method

Localcolabfold (model1_ptm, recycle=6 or CaRMSD < 0.4, centerd and rotated around proximal Cys)runner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -alirunner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -ali -upMaestro (Protein preparation Wizard)proPKa pH 7.0, save as pdbHeme was extract from proximal-ligand-aligned crystal structure of CYP101A1-CYP150A1Heme was added hydrogen using maestro builder, save as mol2ES_scoreMaestro output

runner_P450.py

align_P450.py

CYP201A1

>CYP201A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNIASVRRPI VPPTPPRAPD DMSFLGRVAV IRQNMIATWG QRAYEEDVLE 00500051 GRFFLHKSFI LNRPDAIRHV LLSNYENYTR TPAGIRMLRP VLGEGLLIAE 01000101 GHAWTFQRRT LAPAFTPRAT ANLVPHMTAV LDETIAKLDA RSGETVDLRE 01500151 TMQRMTLEIA GRTMFSFGMD RHGPTLRNFV VEYGERLGRP YFLDMLLPVS 02000201 WPSPMDFARA RFRKRWTEFV AMLIAERRAA GKKDGAPPRD LFDLMDEARD 02500251 PETGKGFSDE QLIDEVATMI LAGHETTATA LFWALYLLAL DPDTQEEVAS 03000301 ETRGEHLDSM ADIDRQKFTR AVIEETMRLY PPAFLIARAA RAKDNAAGIE 03500351 IGRGDIIMIA PWLLHRHEKL WDQPNAFVPK RFMSTEAPDR FAYLPFGAGP 04000401 RVCVGAPFAQ AESVLALARL IGAFRVELVD TVPVIPHGVV TTQPDRSPMF 04500451 RITRR

Alphafold2

201A1_m5.pdb

.fasta

UniProt Q89WX1

AlphaFoldDB

Heme Docked

PDBj

CYP202

>CYP202A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSIAPGITID GPARRVSLDV RNPRFFRNPL PAYAALHAQC PAFFWEEPQQ 00500051 WFFAGYEQVN SLLRDRRFGR QILHVATREE LGMPEPKPHL KDFDALEAHS 01000101 LLELEPPAHT RLRTLVNRAF VSRQIEELRP EIEALSHAVI DGFEKDGETE 01500151 LLKTYAETIP VTIIARMLGI PVEAAPRLLD WSHRMVKMYV FNPSLETEFD 02000201 ANNASAEFAD YLKGIIAEKR TNPADDLLTH MITSEKDGER LSDAELISTT 02500251 VLLLNAGHEA TVHQIGNAVR TILQSGLSPA ELFSDEKATE RTVEECLRFA 03000301 APLHIFQRYA LMDIELENGI ALRKGDKIGL MLGAANVDPR KFSSPDTFRP 03500351 DRNEGANVSF GAGLHFCIGA PLARLELQIS LPILFRRLPG MRLKNEPPVK 04000401 DAFHFHGLER LDLVW

Alphafold2

202A1_m5.pdb

.fasta

UniProt Q92QQ5

AlphaFoldDB

Heme Docked

PDBj

CYP203

>CYP203A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MFSFDPYSPI VDADPFPLYK TLRDEYPVFW SEPAQMWILS RYLDVAGAGS 00500051 NWQVFSSAKG NLMTELPNRA GATLGTTDPP RHDRLRGLVQ HAFMKRNLEA 01000101 LAEPMREIAR DAAEALRGRD QFDFISDFSS KFTVRVLFAA LGLPMGDEQT 01500151 VRDKAVLMVQ SDPVTRAKGP EHLAAYAWMQ DYASSVIAQR RAEPKNDLIS 02000201 HFSMAEIDGD RLDEREVLLT TTTLIMAGIE SLGGFMSMLA LNLADFADAR 02500251 RAVVADPALL PDAVEESLRY NTSAQRFKRC LQSDLTLHGV TMKAGDFVCL 03000301 AYGSANRDER QFPNPDVYDV KRKPKGHLGF GGGVHACLGS AIARMAIRIA 03500351 FDEFHKVVPD YTRTEQQLNW MPSSTFRSPL RLDFAVEQAA SRSAA

Alphafold2

203A1_m3.pdb

.fasta

UniProt Q6NB21

AlphaFoldDB

Heme Docked

PDBj

CYP204

>CYP204A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MARAATAAGN GLPLLDGGVP LLGHLAQFFR DPVSVLKRGY RSKGRLFAMN 00500051 FMGQRMNVML GPEHNRFFFE ETDKLLSIRE SMPFFLKMFS PEFYSFAEMD 01000101 EYLRQRSIIM PRFKAASMKQ YVPVMVEESL NLVERLGEEG EFDLIPTLGP 01500151 VVMDIAAHSF MGREFHEKLG HEFFELFRDF SGGMEFVLPL WLPTPKMVKS 02000201 QRAKRKLHAI LQSWIDKRRA APLDPPDFFQ TMIETKYPDG RPVPDEIIRH 02500251 LILLLVWAGH ETTAGQVSWA LADLLQNPDY QKVLRGEISS LLGGSDGRDL 03000301 GWEQAVAMEK MDLALRETER LHPVAYMLSR KARADIERDG YVIRKGEFVL 03500351 LAPSVSHRME ETFRNPDAYD PERFNPANPD AQIESNSLIG FGGGVHRCAG 04000401 VNFARMEMKV LVAILLQNFD MELMDEVRPI AGASTYWPAQ PCRVRYRRRK 04500451 LDGSEAGADM AALARAAGCP AHT

Alphafold2

204A1_m3.pdb

.fasta

UniProt A4XF07

AlphaFoldDB

Heme Docked

PDBj

CYP205

>CYP205A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIPAIPVLIG DTAMIRFPSP VAIRNLQQLR REPLTLLEEL AARGDVVPFR 00500051 VGPQMMVLVN HPDLIREVLV TQHRSFVKGR VLERAKRLLG EGLLTSEGEL 01000101 HLRQRRLMQP AFHRQRIAAY GDAMVAVAEA RSARWQDGLV LDVSREFMAI 01500151 TLQIVGITLF SADTEADADE VFAAMHDLVA MFDLAVLPFA DWLFALPLPP 02000201 VRRFQAVKAR LDAIIYRLIA QRRANPVDRG DLLSMLLTAV DHEGDGYRMT 02500251 DTQLRDELLT IFLAGHETTA NALTWALYLL AQYPSLAAHL AAELDTVLGG 03000301 RKPTVADLPK LTYTSWFFAE ALRLYPPAWL IGRRAIAPVT LGDVRIAPDT 03500351 IVLLSPWLMH HDPRFFHEPY HCDPLRHTPE AQAQRPKFAF FPFGGGPRTC 04000401 IGEPFAWMEG ILVLATLAQR WQFLPVADHP VVLQTGITLR PRYGMQLQLR 04500451 ERRTVLGAA

Alphafold2

205A1_m2.pdb

.fasta

UniProt A9WIU2

AlphaFoldDB

Heme Docked

PDBj

CYP206

>CYP206A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTEIGFRTPS TDTTGAQPVS KLATARLALS LIRNPLKALP PEIFSEPAVF 00500051 TRLGGVMRVH LADPVLIHEA LVKNAALLGK GEDVRRALGP ALGQGLLTAD 01000101 GDHWKWQRQS VAAAFRHEKL LELLPVMIET ARRTQKRWRS SSTADIDIGH 01500151 EMMRTTFDII VETMMSGGYG IDIARVEQSI TDYLKPTGWT FALAMLGAPE 02000201 WLPHPGRRKS RAAVDYLRAS LATVITGRRK NPTDRPDLVS MLLEAKDPET 02500251 GRMMSDEEII DNLLTFITAG HETTALGLAW TFHLLSQNPE TERKAVEEIE 03000301 AVTGGEPVAA EHIANLAYVR QVFSEAMRLY PPAPVITRTA LQDFRLGEHD 03500351 IPAGTVLYVP IYAVHRHTAL WDEPERFDPS RFEPEKVKAR HRYAYMPFGA 04000401 GPRVCIGNAF AMMEAVAILA VILQKNHLEN RTMASAEPLM RVTLRPQERL 04500451 MMKITQRQNK SPAV

Alphafold2

206A1_m2.pdb

.fasta

UniProt A9CIX2

AlphaFoldDB

Heme Docked

PDBj

CYP207

>CYP207A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKGNLPAMST ATSSSVTGSV TTPRTATTPG RRPGLAPGGV PVLGHLPMIL 00500051 RSRFEFIETV RNAGPVTRVK LGPKTAYFVN DYELLAQILV SDADKFVRGI 01000101 HFKKMRNMVG NGVVTTSGDL HRRQRRIMLP SFSQRRLAMH LPVMRKIMSE 01500151 FVASVPERRP YDLMGPVMGV GCDIVTSTML GEKTPPEVLR LVREAVPVFV 02000201 ENAAIQAVDV TGIYKHLPTK SNRDFERLLN AFNEYMYSVI DDKFRNGAGE 02500251 EAGLLDMLIN ATDPETGEKF DRTEVRDQAA TILLASTETT ANTISWACYE 03000301 LARHPRIFAE CRAEIDALVK DRDWLDIEIG RHDLPALKRV LFEALRMYPS 03500351 SYLLSRQASV DTTLGGYAIP KDAAILYSHY GQQRDERNFP HGDEFDPDRW 04000401 LDKDGAEVTA SAFMPFGFGA YRCLGESVAV LEATYCLAMM VHQWDFALSD 04500451 YSEPKMNATI TLSPKDLEFL FTKRTESGAH DE

Alphafold2

207A1_m4.pdb

.fasta

UniProt Q9AJE2

AlphaFoldDB

Heme Docked

PDBj

CYP208

>CYP208A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRIDPPGPPL RALPGLLRKL AVDRLGMMRD AAGLGDAVRV SMGPKKLYIF 00500051 NRPDYAKHVL ADNSDNYHKG IGLVQSRRVL GDGLLTSDGE TWREQRRIVQ 01000101 PAFKPGRINQ QAAAVAEEAA KLVALLRGHE GGGPVDVLQE VTGLTLGVLG 01500151 RTLLDSNLTA HESLAHSFEE VQDQAMLEMV SQGTVPAWLP LPPQARFRRA 02000201 RRELYRVADL LVADRRSRMA DGGPGDDALS RIIVAADRRR DDPARARNRL 02500251 REELVTLLLA GHETTASTLG WTLHLLERHP EVRDRVRAEA RAALGDGVPG 03000301 PEDLHRLTYT TMVVQEAMRL FPPVWILPRV AQQRDVVGGY TVSAGSDVLV 03500351 CPYIMHRHPG LWEDPERFDP ERFEPRQTAD RPRYAYIPFG AGPRFCVGSN 04000401 LGMMEAVFVT ALVTRDLDLR TVAGHRAVAE PMLSLRMRGG LPMTVSTAR

Alphafold2

208A1_m4.pdb

.fasta

UniProt Q8GME3

AlphaFoldDB

Heme Docked

PDBj

UniProt P00183

AlphaFoldDB

CYP209

>CYP209A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MGTSEPVEPD HALSKPPPVA PVGAQALPRG PAMPGIAQLM MLFLRPTEFL 00500051 DRCAARYGDT FTLKIPGTPP FIQTSDPALI EVIFKGDPDL FLGGKANNGL 01000101 KPVVGENSLL VLDGKRHRRD RKLIMPTFLG ERMHAYGSVI RDIVNAALDR 01500151 WPVGKPFAVH EETQQIMLEV ILRVIFGLED ARTIAQFRHH VHQVLKLALF 02000201 LFPNGEGKPA AEGFARAVGK AFPSLDVFAS LKAIDDIIYQ EIQDRRSQDI 02500251 SGRQDVLSLM MQSHYDDGSV MTPQELRDEL MTLLMAGHET SATIAAWCVY 03000301 HLCRHPDAMG KLREEIAAHT VDGVLPLAKI NELKFLDAVV KETMRITPVF 03500351 SLVARVLKEP QTIGGTTYPA NVVLSPNIYG THHRADLWGD PKVFRPERFL 04000401 EERVNPFHYF PFGGGIRKCI GTSFAYYEMK IFVSETVRRM RFDTRPGYHA 04500451 KVVRRSNTLA PSQGVPIIVE SRLPS

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP210

>CYP210A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MISISKSKQK LLPPGPRSPM ALQTLQWLKN PVPFLEACGA RYGEMFTLKL 00500051 PTQWPVVVVQ HPEAVKEVFA LDSNAGHAGE ANNILKPFLG KYSLLVLDGE 01000101 EHMRQRKMMM PAFHGERMEA YGHAMIDAAH ASIDAWPVGS PFGVHAPMQA 01500151 ITLQVILRTV VGMTDGPLLA ELEALYPQVI DAASAPAMHF ELFRKDLGPW 02000201 SPWGKFKRRS ARGKEIMIHE IRRAREKGTA GRTDVLAMII DAKDENGELL 02500251 TEDEIHGELM TLLVAGHETT ATALCWALRW LLRDAALTRR VAEEAAEVAD 03000301 DPVKIAKSEL LDRVVKEALR LQPIGPVVAR VLKQPLTIQG RELPADVMVA 03500351 PCVQLLHHRP SLYPEPTRFD PDRYATFTPK PWEFIPFGGG LRKCIGAAFS 04000401 MYEMKMVLAT AFSRLSMELA TDDIKIIRRG VTLAPSGGLP LVIRKKSPRA 04500451 TKPIAA

Alphafold2

210A1_m4.pdb

.fasta

Heme Docked

PDBj

CYP2

CYP211

>CYP211A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAGLVMSPVE ALDALGTVQG RQDPYPFYEA IRAHGQAVPT KPGRFVVVGH 00500051 DACDRALREP ALRVQDARSY DVVFPSWRSH SSVRGFTSSM LYSNPPDHGR 01000101 LRQVVSFAFT PPKVRRMHGV IEDMTDRLLD RMARLGSGGS PVDLIAEFAA 01500151 RLPVAVISEM IGFPAKDQVW FRDMASRVAV ATDGFTDPGA LTGADAAMDE 02000201 MSAYFDDLLD RRRRTPADDL VTLLAEAHDG SPGRLDHDEL MGTMMVLLTA 02500251 GFETTSFLIG HGAMIALEQR AHAARLRAEP DFADGYVEEI LRFEPPVHVT 03000301 SRWAAEDLDL LGLSVPAGSK LVLILAAANR DPGRYPEPGR FDPDRYAPRP 03500351 GGPEATRPLS FGAGGHFCLG APLARLEARI ALPRLLRRFP DLAVSEPPVY 04000401 RDRWVVRGLE TFPVTLGS

Alphafold2

211A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP212

>CYP212A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKTPPQSSCP FHAVGRPPTP PRSSAGRWPP GPESGLTGWG LLKLMSRDLM 00500051 GTLAGWQREF GDLVHVRTWP EHQVIVSDPQ LARELLVNQA DALQRWERAL 01000101 TVYRRVHGHS VLIAEGQVWR EKRQALQPDF TRKSVQAFSP SIVEAARRAF 01500151 EQWPARHAAW PIESELTSVT MEVILRMMFS SGVGSEAQQA EEAVHTLMVA 02000201 STEELWRPAS LPDWVPWQRK RRRARLLMNG LIERHLQARL AMPQDAWPED 02500251 LLSRLLRLHL QQPQSWPLQA VRDECKTAFL AGHETVATSL TWWAWCMASH 03000301 PEIQERAREE ALAALSGGGQ ADPAALQYVS QTLLETMRLY PAVPLLMSRR 03500351 ALKPVTLGDW TFPAKTVFMV PMQLMQHDER WFPEPRSYRP ERFGPDAARP 04000401 QQGAYLPFGG GPRVCLGQHL AMAEMALVAA QLLLRYRLSA PEGAEPPRPV 04500451 FHVSQRPSQP LTLGIARI

Alphafold2

212A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP213

>CYP213A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTAAPLPSTG AVTGLGETLA FFRDPSFSQR RFSELGDVFE TKLLAQSIVF 00500051 IRGERAIGDL LKQEDCLQGW WPDSVRQLLG SKSLANRSGA DHKARRRVVG 01000101 QLFSSAALSR YTPAIEALVN DLANELQQAE GPIPLAARMR RFAFSVIATT 01500151 VLGLEAENRD ALFADFEIWT RALFSIPLAL PGTPFARALA ARQRLLARLK 02000201 TVLQTNNNRQ QGGLDLLSGG LDEAGLPLDD DDLVEQLLLL LFAGYETTAS 02500251 SLSCLFRALL LNPEVEQWLM QDLNNHERPS RLDATVLEVM RMTPPVGGFF 03000301 RQNTQSIELA DVAIPQGRVI QVVLSSSSTT NQIDLETFRP QRHLDGSFQQ 03500351 TLLPFGGGER VCLGKALAEL EIRLMAMGLL QRVQLHLEPD QDLNLQLIPS 04000401 PTPRDGLLVR ATAR

Alphafold2

213A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP214

>CYP214A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNMNFKSSNP ASSLALPPGD LGLPFIGQNK KIFKNPQNFI EEVYQKYGPV 00500051 YKTNFLGKNF IYFQGYEAIK FILTNENKYF TYSQILRNYQ RIFGENDITV 01000101 LAGKEHRERQ KILAKTIKSK NLNNYIDIIH DLSQSYFLKW IKSDYVDLYS 01500151 EINNYTLDMI LKLLLGIDYA SKSEISNYLK DMSSGLNTIP VVFPWTKFGS 02000201 ALESKNKLFN QFEQIIVRRK KENNFGSDIL GILLTVQEQM NYELTPREIV 02500251 GQMVNLLSLG KKELSSALSS FFILTSEHLD VLKLLQIEQE KMDVSEPLSL 03000301 DKYKKMVYLE QVIKEVLRLV PPVSGGLRKI IEDCSFQGFR IPKGWHAYYY 03500351 ISSVLKDPEI YKQPEIFNPE RFNPTNAEDK KKPLCYIPFG GGARECIGKE 04000401 FAYLVIKIFI SALLDNCSWK FKENQDLTIN TFPVARPAHK IEVCFTPK

Alphafold2

214A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP215

>CYP215A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPAPIRIQQC PVSHTDFSTD TEIYGHYAML DAEREASRFR FNDTTDRGFL 00500051 MIQRYDDVVE GFQHHETWTT KVRSAINPDS RSGTALLPQD LNGEAHAKLR 01000101 RVLNPFFSPA AVRRMEPMAV ARCIELIEEL QPKGSCDFVA EFAIRYPTDL 01500151 FLALLGLPVS DGEFFLPWSE TVFAGFFGED PAKVAEARKN IIDYFTETIR 02000201 ERRANPRDPK EDMVTRLLEA RIDGTPIPEE DILTICLTLM LAGLDTTRSA 02500251 LGYIYAHLAR NDADRQAIID NPDLVPRAVE EFLRMYPLVF QVGREVQEPT 03000301 EFHGLDLQPG DVVWLGIAQA NRDPRKFPEP DRFNPDRKGV NQHIAFGAGP 03500351 HRCLGMHLAR LELAIVLREW HQRIPHYRIR EGVTLTERGG QLTLPTLPLE 04000401 WEA

Alphafold2

215A1_m4.pdb

.fasta

Heme Docked

PDBj

CYP216 !!!FW!!!GL!!!

>CYP216A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTRSPSPPIR LYQAADPTAL WPALLAEYGE IAPVELEPGI NGWLLLSWRL 00500051 NREVMRDAET YARDPDRWAD MANGRIGPRS PLRVLYGKYQ SALYSDGADH 01000101 KRYRAALTAA LDSLSLRQVT GHITAAADQL IDAFCLHGKA DLVTQYTRQL 01500151 TVTVLARLFG LGPAETMRIC LEMQAMWDEG PNAFPALMRI AEMLTELARK 02000201 RRRTPGKDLA SVLVAQGLSD VEVRDNLLLI IAAADDPVTH LTGNTIRMLL 02500251 TTPESRAALT TSPGLISEAV NTALWTTPPL QTLVGRYPVR DVELAGVPIR 03000301 AGECLVLGFA AASLDLAQHS DADTMSTNRA HLTWGVGPHS CPLRGQDMAL 03500351 AIAETAVERL LRRLPDVALA VPPEQLRWRP SLAVRGLESL PVRFTPTPVS 04000401 NPGGTEWDSP ADPTPSTSIR PKESTSPRSD ANSSTKARWY AWLFPGTWKS 04500451 GL

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP217

>CYP217A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTLAPSTDL DFYSEEVILN PYDTYRRLRD TAPAVWLERY QVWAVTRYDD 00500051 VYAALHDHRT FASGYGVALN DPLNEKMKGS ALVSDPPYHD HVRGVMGGPL 01000101 TPRALRKHHD YFQERADALI DHLLELGRFD GVRDFAQIFP LSVVPDLLGW 01500151 PAEGREHFLD WASAGFQALG PMNERALRDL PTLKGMWEYM AEIVRPGRLA 02000201 PGSWGAALVE AAEKGEVDKQ LLPALIGDYL VPSLDTTVSA LSTMLWQLGE 02500251 NPDQWRMVRE DPSLIPNVLN ESIRYEAPIR ALSRYVTEDT EIGGIPLARG 03000301 SRALMVYASA NRDERHRKDP DTFDVTRSNA NTHVGFGHGI HGCVGQGLAR 03500351 LEGHSLLNAL VRKVARIEVG QPTWRVHNTI RAMTTLPVEF SA

Alphafold2

217A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP218 !!!FW!!!GA!!!

>CYP218A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MILNCEHDPV FHVPVVSTPG RWSVTHPLYP VGNSDTPHDR NAGSSEGCPV 00500051 SLDDREALHR LAVPVYGELD ADLPDVLERL RKQFGRIAPV EVAPGVAAWI 01000101 LLGYDVNRRV LQDSAEFARD PRRWREAREG RATPEKVPGP FWYFRNALAS 01500151 DDPDHSRYRP VIVDALSGIT ADGTRLAVRR IATELLAPVA LTGKADLVAD 02000201 FAFRVPLLVL NRYFGLSAEE GLELVELMRQ VWDGGEEAEK ARLGLFAYAQ 02500251 SVTARRRENP GSDIVSRMVR HPNALDDEEI AHQLILLISA AHDPLMNLIA 03000301 NTAHTLLTDE EVRYDLAGAH LRVEEVVDTV LWRSPPITLL PGRYPVRDTL 03500351 LEGAYVQEGD CLIIGYGPAH ADPAVAPYID PLSPSGIRGH LAWGTGPHAC 04000401 PAQRISRQIA VDAVSALLDL LPDVRLAVPP ESLERRRSLF AHGLKALPVT 04500451 FTPVDITPPT EAPWQQSQNP SSSSQENPAS KPSSSVKRGR LPEWWSKVWK 05000501 FGR

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP219

>CYP219A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEAEAAIPPL DTSDPALIPD PWPTFTTLRE RDPFHWSKYG YWVVSRHEHV 00500051 RDVLMNRKDF GTGDFAANLR LFYGPDFDVL ANPAYRWLSE VFIMQDPPQH 01000101 TRIRNLVVGS LTAKRVRAME PRIREIAQAL TDGFKARGSA DLITEFAYKF 01500151 PVMVICDLMG IDYEASEMAD LIAAIPEAFT VFEARILSPE ELALANRRIL 02000201 ELEAFFQAQF ENRLAHPRGD LLTSLARTGQ EPGGLSVHEA ITVTIGLFGA 02500251 GFETTANIIG NGLHALHANP EQWARLVADP SGMASGACEE ALRHQSSLIA 03000301 TYRTALADTS VCGHPVSAGQ RVLTLIGAAN RDPRKFADPD RFDIARNDAD 03500351 HLTFGGGIHF CVGAELARIE ARVAFEHLAR ELPQMQVDTG GACWRENFLF 04000401 RGLTGLEARW PAQA

Alphafold2

219A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP220

>CYP220A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTFIYDPNDP DVRRDPHAVF RKLRETEPVH WSPKLSGWVV TSYELASEVL 00500051 TTNGTYSAER FTAVQQHLSE EKRVTAAEVM RWFQHWMVFR DPPDHTRLRR 01000101 HMANTLNIPV FDARRETVIS VVNELLDRIP VGDAFDFFQA FSLWMPGIVV 01500151 ADLLGVERDR LLEVKQWSDD MMTFIGSARG VPDKYERARR GANGMGTYFL 02000201 DLIAKRRAEP REDALSRLIA SEVEGQRLSD DELVGCMMMV LNGGHETTAN 02500251 LLNNSMLALA AHPQTVQHLR QHPDEMAAAV EEFLRYDSPV LSIGRIVTED 03000301 TELGDQEIAA GDRVFAMLVG ANRDPEVFSD PDELRTSRNP NPHMAFGKGP 03500351 HFCLGTPLAR LEGQIALTAI LERFSSIELC EPVESIPWLN SLVTHGPTRL 04000401 PLRLK

Alphafold2

220A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP221

>CYP221A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSDPLLKLLQ KPLFDPEKRH RVSLREYMDL NIDRMRAIIG NGLMTNAMWL 00500051 SQPRQSEFRL MLERAALIGA VDYSLLACIV DHFIAGDAFF AHGSQHQIAQ 01000101 YHQEICQLKA VYAFGCTEIA SGSDVANLQT TINYDPHKHC LILNSPTPQS 01500151 CKFWIGNALH AAVVVMVLGR LIVKGVDEGL HWFRVRIREQ ENGPLLPGVR 02000201 ITTCDPKGGI HANQVAGIRF CNMKLPLDAL MQRYARFSAQ GVFSSEIPPK 02500251 ERLKSAMQTF IQERLFLIAG ARGAASMCVY LAYRFACHRL VKGNEGSQSL 03000301 LTKALFRQRL YAEQLKVLAL KLLEQAVLSR FEACWHQPAR RKELHILAAV 03500351 VKSVGTWLGL EVMSACRELC GSQGFHHHNR IVTLVMDHGI STTFAGDNNI 04000401 LCCQVARDAI NRPRFANENI AQRIESLIVD QCRRAGDFSH RQAVALTYAR 04500451 ALDLIINEGK HHPLVTSEIF EDIVHVFSPK LYEWELVAST KLEQNATEAQ 05000501 LILLNELLKP PSELVRAPID KKNYVKHFTK PLYDNKPDFS NRNTIRNPYR 05500551 AYTWLRKHQP VYWCEHLQAW FLTRYCDVIA AQADSRRFSS NRMQQLIDAR 06000601 VPENKRTHLN EFIKLASRWM YSQDGDTHKA SRHLLGNAFT PRSIEALRAI 06500651 IQDITDRELS RLHGQTDLKT ALFDRVPALI LARLYGMKDD EALRLRRWTR 07000701 DIVMFLGGSQ DADQGPDQAL EGIKEMYACF AELIEQRRRQ PGDDLVSRVL 07500751 ESGQNSAASL DEVLAQIVFI LVAGYTTSAD QMCLGLLHLL KHPQQLEALL 08000801 ADPTLIGSFI EEMLRFDPAG SLSHRILMED VTIDNITMKK GNLVYLIRAS 08500851 ANRDPEKFHA PNTFDIRRAR NEHLTFGKGE HFCMGTSLFR LEAEIVFTSL 09000901 LKRFPDLQLI ARRPAKWRNS NLQFRGLKTL PVDLGTGV

Alphafold2

221A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP222

>CYP222A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSLRGLPGTA PRQRAGSPGP ASFLKRRRDA APRPTISLDG DGPLRAGLGS 00500051 IPGRRLQFGA HLFVEHVGVP VFFARRKHVG RGHVAHTVPL ALLSVDSDAH 01000101 VALLRDQRSL FSLAILTLML LSITIITRTA IKITHHTRSR HRPYPHSSQR 01500151 TSAHEPGQER AHSMSNPTRC PVIHFDHHSP EHAKDPVGAY RALRQTHKRG 02000201 WTEAHGGYWV LSDYQSVFDA ARDDDLFSST REAAGTDGLA IVIPPTPMYH 02500251 HIPIEVDPPE FRKYRKIVNQ VTAPAAVERM KEMVERYTVA FVDSVIEKGE 03000301 CDFTTIIGVP AIVTIDWLGL PVKDWKRYAD AHRATLAEPQ DSEAFRHAVE 03500351 VELPALSQQM WDTIRARRQE PKDDVISFLV SQKVDDRPIT DEEVFAMVDL 04000401 LVSGGTGTTA SLVSQALVWL AKNPDVRQEL IDDLSLLDRA VEEFLRVFSP 04500451 TQALARTVTR DVEFHGCSMK KGDRVLLSWA SANRDEEQFE NPDTIDIHRW 05000501 PNRHVAFGIG VHRCAGSHLG RFMAKRLLQE ILTRMPDYTI DFDALVPFHD 05500551 QGTNVGFRSI PAKFTPGKRV LPLSEAVIG

Alphafold2

222A1_m1.pdb

.fasta

Heme Docked

PDBj

CYP223

>CYP223A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MHRAMTTTVQ DFDPEVPEDF DSPHAEYARL RRECPVAHTN GLGGFWALTR 00500051 YEDVKRAASD STTFITSVQN VVPKVAFTGR RPPLHLDPPE HTPYRKALNP 01000101 LLSLERSEAF AGKARELTRK LLAPMVENGG GDICVELSSY LPVHVFGEWM 01500151 RMPEEWLDTL HDAGRAFILA VHSNTPERMK ETSLRLYDMA RGLIAVRREN 02000201 PQDPALDPTS ALLAARHEGE PLPEELLVGT VRQVLVVGMV APMVMIGNIC 02500251 VHLSRDKALQ QQLRADPSLV PAAIEEFLRL YTPYRGFART AVCDVDMGGR 03000301 TIPKDEAIAL VYASANRDED VFPDGDKFIL NRPNIAQHLA FGRGPHNCPG 03500351 VHLGRMQLRV ALEEILAATR EFELSGPVSV SRWPEVGALS VPLRFV

Alphafold2

223A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP224

>CYP224A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTLLFQPSPP DHVPGERMVD FDMFHVPEGQ DDPVEIWHDL VRRGVPRIFY 00500051 TPRNGGHWVF LDYADIVEAY RDHTVFSTYQ TPVPPIEPFP VVQPQGVDPP 01000101 AHNVFRRLLA PMFTPTAVRG MIGELERRAS ELIDRFAARG ECDFITEFAE 01500151 RFPTSTFLHL FGLPEEQLDA FLALANVFFR STDAETRARN IGEIYAVLDT 02000201 LFREKERNPG NDIASAIVAA RDEEGRQHPW EDILNCGFLL FVAGLDTVTN 02500251 TMAYIWRYLA TTPAARRHFR ERLDDPDAFL RAIEELMRIN AVSNLFRRVT 03000301 HDCEYKGVQL RRNDRVVLPN TVANRDPRVF SDPQAIDLDR EVNVHLTFGV 03500351 GPHRCIGSVL AKREVMVSLQ QWLRRIPEFE LAPEQPAGSA FGGSVMGFTA 04000401 LRLRWVRVEA

Alphafold2

224A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP225 !!Q insertion !!

>CYP225A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPSPPRLAGT STASGLEDQM QFPFSRSTNP NVDLSSLDAF NEGAPFATFD 00500051 RMRREDPMAW SEMVNGDRGF WSVTRHADLL ELNRQADLLS SAKGIRMEDQ 01000101 TEEEYEARKT FQETDAPHHR GFRALVSKAF SKGTVAGFED QIRKIVTDLL 01500151 DVALAEGEFD AVDRIARRLP MQMLAQIMGV PQEDGPWLVE KGDALISNSD 02000201 PDYTDFVVDQ VDTEAYRMLP FRSPAAVELF DYANGLLDRM DAGEQIGVLN 02500251 LVREPTSTGT RMSRDEFRNF FCLLVAAGND TTRYSISATI HALANNPHLL 03000301 QALKDGDFTS WEAAADEMIR YASPTTHFRR TATRDFTFHD RHVKAGDKVL 03500351 LWFISGNRDE TAILDPYTIN LRRERNPFLS FGQGGPHICL GMWLAKLEVA 04000401 IVMQELAKRL SSIEQVAEHS YLRSNFIHGI KHLPVRIVAR

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP226

>CYP226A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 METGMTSPAK SNALESAFES TASNYRGSDV DLNAIYRDMR RNSPVIAQDF 00500051 MASLGVPNIA KLDPNRPTFT LFKYKDVMSV LRDAANFTSG FIAEGLGSFF 01000101 DGLILTGLDG EAHRRARALL QPVFLPEVVN RWRESKMEPI VRNEFIGPMV 01500151 PQRRADLMHF GLHFPIRLIY SLIGFADDRP EQVEQYAAWA LAILAGPQVD 02000201 AQKAAIARKA AMEAAEALYA AIRSEVAVVR AKGAEGEDLI SRLIRAEYEG 02500251 RRLDDHDIAT FVRSLLPAAG ETTTRTFGSL MTLLLERPAL LERVKADRSL 03000301 VSKAIDEAVR FEPVATFKVR QAAVDTGIGG VSIPKGAMVQ CIVSSANRDE 03500351 EVFENSETFD IDRKPKPSFG FGFGPHMCIG QFIAKVELQV AVNAILDLLP 04000401 NLRLDPDRSP PKIVGAQLRG PDAVHVVWD

Alphafold2

226A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP227 !!FW 2aa!!

>CYP227A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVNIMTLKD KVLKGQDRQL WLAPILAKVG YDTAIGKFLR LIFYYTDASI 00500051 ILKAWRDFLY IRKDNIGDKY FAVDQAIMHS SHSQVRELMQ TQPQLRGNDL 01000101 GIIRILAPSY LLDNPLSLGT NGNEHTGLRT VILQALPEPS QKIDFLGNLV 01500151 EQSLLEAAKQ GKLHIGNDLP KIILSILHQL VFQISLSEEE ITASDSYIKG 02000201 LALASLPNFI NKYLLAILTA PKIRHRQYLT NRYKQSAKWA SYFETGAQYQ 02500251 LNEHQIANTL FDMIHIAGTA GTSALLGSVI GVLCLDNDLR NDVVSEVNAV 03000301 WNGKKTLDPD ALEQLTILNQ VILETARLYP PVRFVSQLTN EGGEVEIGEQ 03500351 KCPFQKGTRL LGSIFTANRD ANRYQNPNDF DLTRNFSDIL SWNGEGHERA 04000401 CPGKSLSIGF IKIFCLHLFQ NYQWDSITEV KWDFEKVTAV TPNNLVLQGF 04500451 AQRL

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP228

>CYP228A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MGPRPHARLV RRPPLPARAR GHPVTGTALA PAPAGLAAAM RWEERVQRGA 00500051 HPLVYPAIRA LRHRGPVVRV PGIGVVVSDA ATARAVLLDT EHFSKVGPGS 01000101 PSDLWTPVLG PSVLLNMEGA DHARLRRALS GLFTPRAVRD LVAVSVPDVL 01500151 AGLAPRLLAG ERVDLVAETA TMAGTVVCAM TGLPPTDSAV REAMTAAQSV 02000201 VGLVRLHRRS LTPSQVRHAR AVLARLSAPA RDAYRAGDPA TVPGRMRELG 02500251 LSEDEALGAV GAFVLTGTET IQSFVPRLVA LTADTGWLDR LLAADPGAGP 03000301 EAAALRGRVV EEALRVTAPT PAMLRSVRAA TTVGDVRVRA GDRVVIATIS 03500351 CCKDAGPFDP DAPVDPAVRH LWFGAGPHFC LGMPLATAQV DAVLDALRPV 04000401 AAAGRSLQVT DRAVARGVLI PAYRSLV

Alphafold2

228A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP229

>CYP229A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDPIIAATHA DPYPYYAELR AAGGLTFHHG LKLWVASSAR AVCAVLAHPD 00500051 CRVRPVQEPV PKAIVDGMAG KVFGLLMRMN DGEAQRCPRS AIEPPLGLID 01000101 REEVGALVSA RLITNDSDGL YKAMFRGPVC VVASLLGFTP AQARVISELT 01500151 ADFAACLSPL SNDLQLAAAH RAAEQLRGYF IEMLADPNPF LADIRQRFVG 02000201 NEEVLLANLI GLCSQTFEAT AGLIGNALVA LHRQPELRNA SVDSLLAEVQ 02500251 RFDPSVQNTR RFMANSCEID GVRLEAGDVI LVLLASANRD PALNENPDRF 03000301 RVDRPNRRSF TFGSGRHQCP GQTLAMTIAS ATLTEILARN IDPGRFTWHY 03500351 RPSLNGRVPM FSEVQP

Alphafold2

229A1_m1.pdb

.fasta

Heme Docked

PDBj

CYP230

>CYP230A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTSWEREVSR GAGNRQLPVV KGWPLLGSAL ALIRNPLGFL QTTRSTYGDV 00500051 YRVKAAHMNF VVLAGMEANR FVADKGKDCF VSSGFWEATL QEMQCPHSFI 01000101 GVDGDAHRFQ RNLMKPLFSK SAFNERIPML AQIFTDTLQA RYGVDQKVSA 01500151 LFRHVLSQQI GGSLQGYQPT PDEVEALMRY QNTAMNVCAL KKWPRLALRL 02000201 PGYRAAKKQV QALADRIIES ERTQEQTQGY FQTLKEKGQM VQPQWFTPGD 02500251 MRNHAIISYL AGIDTVGATL SFMLLELFKQ PHLHQALRDE VDACFSQGLP 03000301 DADGLENMET LKNFIREVMR LYPTAYAVRR TRRKDFEFQG YSIDKGQDII 03500351 LFTTANHTDP AWFKNPQVFD ITRYEEPRLE HRASGAWAPF GRGPHTCIGA 04000401 GLANILLSLN LALFLYHTDL RPACKLSDIK MDFSNPAAGL SERFAISFTP 04500451 RNRP

Alphafold2

230A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP231

>CYP231A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEHDVFQYYR KMRKESPVHF NNDTGSWDVF DYKSVYFVLM NPDIYSSDPS 00500051 YAGNIPENRQ GPGASFITMD NPDHKELRNV TTPYFLTSKI TGYRDMIEST 01000101 SKRLMEGINK NSDFIRDYAV MLPVTVISEL LGVPENDRSK FKEWSDYIIG 01500151 NRSDAGFQDL NRYMYSTMAE IFKTNTEDNI ISTINKGLFH SEPLSINQKI 02000201 GYVMLLVIGG NETTTNLIGN MVKVLSEHPE IADKLRQEPE LKKGFIEETL 02500251 RYYSPIQFLP HRFAARDSVL NGQEIKKGQR LSIWLGSANR DGAKFEDPDT 03000301 FNMERQNNDH LAFGMGIHMC LGSPLARLEA EIALNDILNK FKHVKINAEK 03500351 TSMLKNPMVY GFSTMQLDD

Alphafold2

231A1_m4.pdb

.fasta

Heme Docked

PDBj 2.5Å

2rfb

CYP231A2

CYP232

>CYP232A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEIPTYKEEP FEWYREMRKN SPVYREGNMI HIFKYNTISK ILSDHQNFSS 00500051 QFRDLLGEEM AAMLNEKTTP SILLLDPPLH TTLRGLVGSA FTPRSIELFE 01000101 PRIREIARML AHAIVEKENS DIVSDLSYQL PIRVISEMLG VPESDSEIFR 01500151 DWSDKLATSL GRGPDIETQY DMADYFYKKI DRNSKGNNLI SRLSTVEMDG 02000201 RKLSDKEIAG FAILLLVAGN ETTTNLITNA ILSLYDHPEI YNEMRKTPSL 02500251 IPGVVEETLR YRSPVQSTRR YSKIDTEIEG EEILKNDILA LYLGSANRDE 03000301 EAFEDGESFN PYRKEKRHMA FGQGIHFCLG APLARLEARI ALEEFSKAVP 03500351 GFEIEKPSPD DRIDSDIMYG FRKLNLKVNR S

Alphafold2

232A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP233

>CYP233A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSALPPPRFN PFDSEFRQDP YRVYAHLRVA APIHRSLGMW VLTRYADVLA 00500051 VLKDPHFSSS QIPLAVRQRS ERPDQAQSHP LARLAAKSIV FTDEPDHTRL 01000101 RHLVVRAIKR RTPEQEQAHL TRIASALLER VGPKGRMDAV ADYAERLPLQ 01500151 FMAESMALPP DSWQTVRDWT HQLRYLLEPG LMGRGDFERV QAVLDEVIAF 02000201 FEDMLAVRRQ QPGDDLISAL DAAHREAQAD RLSDEEIVYC CIMMFVAGHE 02500251 TTRSLIASGL LALLQHPEQL AYLRMHPERM GAAVTEMLRY ESPLQQTKRR 03000301 ATAAVAVGGR TIQPQEQVLL CLGAANRDPA RFEQPDRFDI TRTDNGHLAF 03500351 GQGMHHCLGA ALAQMEAQVA LRVLLERFAN LTLQDTPEWL EHSFILRGLK 04000401 TLPVQWDR

Alphafold2

233A1_m3.pdb

.fasta

Heme Docked

PDBj

40.99% CYP109B1

CYP234

>CYP234A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MMNVLINEYK KKMDSVRLGD PERKGFFYDA KQAIWHCYSY DICSYFLNSD 00500051 YVTKKKLSIP LEIFSASDQS RVARFILYLN NSLIFNDDKY NTDAVSFIRG 01000101 KFNEMNFEVI ANDLLSPLKQ CDLLTAKHLR GVNNLLAASL VGLKASAFFS 01500151 AHALNVGMFF DGSMSGRAHF VSIAESFIAI YQQVLRQITI NGGAEDVIHI 02000201 EKFVADLSVT FIAAHETTMQ LIIATFLYIK SHVITVTENN IKSIVTETYR 02500251 LSSPVLAVNR VFKERLIYKN SCFNKGDRVL FYTGLANFDA TVFDHPYQFQ 03000301 LDREGCPLSF GVGVKKCIGM NIAIHFTCQL ITKILSCYQL DDVEIHEVTV 03500351 GSLAIGCSKF TLKISKK

Alphafold2

234A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP235 Cys was not exist (Non-heme protein)

>CYP2A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEDSELGRRL QMLRGMQWVF GANGDPYARL LCGMEDDPSP FYDAIRTLGE 00500051 LHRSRTGAWV TADPGLGGRI LADRKARCPE GSWPVRAKTD GLEQYVLPGH 01000101 QAFLRLEREE AERLREVAAP VLGAAAVDAW RPLIDEVCAG LAKGLPDTFD 01500151 LVEEYAGLVP VEVLARIWGV PEEDRARFGR DCRALAPALD SLLCPQQLAL 02000201 SKDMASALED LRLLFDGLDA TPRLAGPADG DGTAVAMLTV LLCTEPVTTA 02500251 IGNTVLGLLP GQWPVPCTGR VAAGQVAGQA LHRAVSYRIA TRFAREDLEL 03000301 AGCEVKSGDE VVVLAGAIGR NGPSAAAPPA PPGPAAPPAP SVFGAAAFEN 03500351 ALAEPLVRAV TGAALQALAE GPPRLTAAGP VVRRRRSPVV GGLHRAPVAA 04000401 A

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP236

>CYP236A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKKDLIPDPF EKTREAAGYG EMNDQNDPVT MILRLKDVRK CAHNFKTFQS 00500051 GARPGRIVVP SEVSIRDTRQ IPFEVDPPEH TDYRALVEPW FKRPLEAEYR 01000101 EKLSQQIGYI VEERLARDAV EVVEEFSLPL QSRALTLLLN IPIEEAETWI 01500151 KWGTHVFRSE DSPVDGDKAK ILYDYIDQQI DRALEKPGED LYTVLLNSEI 02000201 NGKKLSREEV KGVMILTFAG GRDTVINAVT NSVAYFAVHP ESLELLRKEP 02500251 EITGRAVEEL IRYFAPLTHM GRVVTEDTQV CEYAVKADSR ISLVWASANR 03000301 DSSVFEKPNE VVLDRKINPH VSFGFSHHNC LGATHARQIM HILLKTLAEK 03500351 VGSIEIQEHE DNIETWGEFE RKVGYDRLKV QFNPLQ

Alphafold2

236A1_m1.pdb

.fasta

Heme Docked

PDBj

CYP237

>CYP237A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRNGTSYLQN FFPGYRRLSR LPKQCLAINP FAGDCMPSRV RLLAPRSDRP 00500051 TQPFPHRWNY EDPVRILETY FWKADEEQGP GRHNRYLDVP GFAPVLVTRD 01000101 PGMIRAIATA TGDREGQFDR DTLPSVGIAR ATGTDTLLYA NGAEWKKQRK 01500151 IAACPFGKTT LFQPEQFCEF ADTFRETVRG RIDVLRQHLT ASGKKTVDIQ 02000201 LEPEVKVVML EMLTNNFFGA DISYEELREK YVPALERVID HIVKDTVKNR 02500251 LGIPWRKFPS VSDRIVRAKA DDATFEELTQ RILVPRGEKK ALWKQFKSDA 03000301 PDAKLISNLK VFLAGALEAT TSYATWAISH LARHPDAQEK VFEEVKDIDV 03500351 YTPEILAGAK YLRAVLDETL RLTPSLYFLP RRATADTWVT SADGRKMFIP 04000401 WGTHLLLDVW HANRHEDHWG VQVSGYPANE FEPDRWRILA EWGRATKDTL 04500451 HFGFGHGPRV CPGKHLGELE VGLTVGALVK TFRFQSESPE NLARAGVSTK 05000501 PADGTRVCMS LRLS

Alphafold2

237A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP238

>CYP238A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEILDRPQAP SDFNPMSEQS FRDPASICQR AREETPVFFY APLGVWMVTR 00500051 REDAERVLSE WETFSSLANS PNVPEEFRSR FAPSVMADSI VAIDPPRHTQ 01000101 ARNVIQRGFM KPKIDPLEPI IEQRAHEIID RFAGESGTEI MNNYCLELTT 01500151 RTLMALYDLP LEDRPMFERI RDVSIKVLAS VYEPMQEPEK SRVWNEYVSG 02000201 YEYFYQLVEQ RRNSDARDII STMASQKDNQ GNPALSTERI ALHLVEIAFA 02500251 GTDTTAQMMA NAILFLDSHP EALAAAKADK TLWSRVFEET VRRRPSAPFA 03000301 GRITTTEVEI QGVKIPAGSP VWVSLAAANT DPRHVGCPMN FDINREAPQD 03500351 HLAFTKGRHT CPGAPLARLQ GATGLRVLFE RLPELKVVPD QPLNFAPMAL 04000401 LPVRLSLQVI W

Alphafold2

238A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP239

>CYP239A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKDVNEVARN FDFHGEALDD IFDTYSTLRH GCPVGRSENY GGFWFLTKSD 00500051 DIFAAEQDPE AFSVYPSMMV PSVSEGIQLP PIDIDPPEHT AYRRILLPLF 01000101 TPQELKKLEQ PIRDTARKLA EEFAKEGSGA DASYHYSRPL PTIIFSRLAG 01500151 YPEQDWPKFD KWVDDIIYER VEKPEVANQA SKDVFSYFEN LLDNWKDDSE 02000201 SANLIDYLCR AKINGRPLTR DELLRYCYLL FLAGLDTTAW SIRAGLWYLA 02500251 NNPADQQKLR DNPDLIPLAC EEFLRTLSPV QVMARTCLKD TVIRDQEIKA 03000301 GERVMLVFGA GNRDEEVFPN PDKIDIERQE NRHLAFGGGI HRCLGSNLGR 03500351 RELVVGIEEF LRAVPQFKPA DPSEKWHGVG PLKLAF

Alphafold2

239A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP240

>CYP240A1v1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIADTARQHR GDIMQPADPL EAVAHPDPYP YYAALARERP FYHDDRLGLW 00500051 VAAGPQAIRA VLTCPAARVR PPGEPVPAAL GAGPAAQMFG RFIRMNDGAV 01000101 HERLKPMLTA YLTQRTAADL AEPAWPAIGN DPAQVDRYLY QAPVHAQACL 01500151 MGLPDEVAAS CAREIEAFMA ACRPGADAAA VARADQAAQA LQARMLAHLR 02000201 AARGDAALGV LRRLALAGGV EADALAANLA GLLLQSCEAG AGLLGNALVH 02500251 AGRLSPAAAA AAPDLLHTCV EIVTHVARHD PPLHNTRRFL AAPATLLGQS 03000301 VPAGAGILVV LAAAHALAEG AWPWTFGAER HACPGRTPAL LHAAQALAHA 03500351 LRHGVDAPAL ARRVRYRPLP NARVPRFHFP PGDTP

Alphafold2

240A1v1_m3.pdb

.fasta

Heme Docked

PDBj

CYP241 rename to CYP152 (4aa insertion)

>CYP241A1X=CYP152M1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKEVPVVDIK ITDLKKLYQK GYNMLEELRH EADAPVVKAK IFNKEAITIY 00500051 GSSAAKVFYD PRNFKRKGAM PKLVLKTLFG QGGVQTLDGA AHHHRKNIFM 01000101 DLMTPERMED YHRILDKNLT QALEAQHGQF ELFDLSKMVF FTSICEWAGI 01500151 NLSAISKDEV EKLAEYQISM ISGTFTSPID HIKGVENRKK SEKWAQGLIE 02000201 EARQNPVAGK ENVALYAFAN ATDLDGQLLP LEVAAVELLN IIRPTVALTV 02500251 WAALMGHALF SRPDLYQQLK NDFSTLQDPF IQEMRRYYPF FPMLPAISLK 03000301 EVEVDGYRIP EGSWVILDLY GTDHDERTVE APDSFMIKRY VGKAKDISYK 03500351 EEYEMIAQGG GNFRQMHRCA GEWITLHSLR VFSDQLVNKF EFSVPEQDWT 04000401 IPFNQFPTYP NSRALLYKN

Alphafold2

.fasta

Heme Docked

PDBj

CYP242

>CYP242A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MADLETKFPR YELISSGKYV DRIPELHELR EKSPIAWVPV MDAAFLTRHA 00500051 DIVRVLKDHR MAPANLTQGI RLLSPEQQEE LEPLSSAVKK WMGHTVPADH 01000101 QRFIGLLKRY FTPAMIDRMR PRVRQLSHEL LDAVEPAGRM DIVSDIAYPL 01500151 PACVIAEMLG VPMDNRAQLL AWSADIGAIA EIVSYDRLME CQRSLLAMQD 02000201 FVLEVVKERR AEPKDDLISM FVAAEREGLV SEAEILSNCV MLLFSGHETT 02500251 GGLITSGLVQ LFDHPDQLEL LKSDPDLMPG AVEEMLRLAG PASVISRVST 03000301 EPVEVAGSSI RSGAAVPPGA DGGKRDPRVF EDPDRLDATR RPNDHLAFAT 03500351 GMFYCLGAAL ARMEADEFFR ILLDRFPDVN PGYETPDWQP VLLISRRLKT 04000401 LPVNLRGVGG SGAGDE

Alphafold2

242A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP243

>CYP243A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTPTRSTSTN WPSTPNCGND VRSRGTRTTA GSGSSAATTP SAKPPQRRHF 00500051 RPQSTSRTPQ TAWTTRARWA SRDPEGQPAL GLGEVDGPYH QALRHALAPF 01000101 FSPGAVEKLN PFMEQSAHWF LDQQITTGQM DLVLDYASPV PAILTMKLMG 01500151 LPYDNWRLYA NLFHSVMAVS QDSDEYAAAI AKVPAMMHEV LDYAATRRAK 02000201 PEEDLTSFLI RFEFDGHRLT DEQLLNILWN LIGGGVDTTT SQTALTVLHL 02500251 GTHPDLRQQL IDHPELYRTA TDEFLRYFSV NQTLSRTVTH DVVLAGQRLR 03000301 KNDRVVISWL SANHDENEFD RPDEIILDRA PNRHVAFGLG PHRCIGSHLA 03500351 RLMSEVMVRA VLXRIPDYQV DVENVHQYLG NPSMTGLGQL PVTFAPGKSR 04000401 KTLRPW
>CYP243A1v1 from Mycobacterium avium subsp. himinissuis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MATRPNVSPD AIVDFDHHSD AFNLNELAVN AELRQRCPVA WNENYGGFWF 00500051 LSSYDAVSQT ARNGDTFAHK YEPNAADGVD YQGEMGVPRP EGQPALGLGE 01000101 VDGPYHQALR HALAPFFSPG AVEKLNPFME QSAHWFLDQQ ITTGQMDLVL 01500151 DYASPVPAIL TMKLMGLPYD NWRLYANLFH SVMAVSQDSD EYAAAIAKVP 02000201 AMMHEVLDYA ATRRAKPEED LTSFLIRFEF DGHRLTDEQL LNILWNLIGG 02500251 GVDTTTSQTA LTLLHLGTHP DLRQQLIDHP ELYRTATDEF LRYFSVNQTL 03000301 SRTVTHDVVL AGQRLRKNDR VVISWLSANH DENEFDRPDE IILDRAPNRH 03500351 VAFGLGPHRC IGSHLARLMS EVMVRAVLDR IPDYQVDVEN VHQYLGNPSM 04000401 TGLGQLPVTF APGKSRKTLR PW

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP244

>CYP244A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTDMPVDPGP FDCMPELLAA ARVAPVVRIP YLEEHAWVVC DPELVRTALT 00500051 HPKMAKDITL VPQFMRKPGL MVGSQPPPEY ARAMIMSDGE DHARIRRVHQ 01000101 PVLSPRNTQR WGERVGVKVG GFLDELEQSR ASDSAEVDVV TDYTHRVPLA 01500151 FISEMLGLPL EAERRLRSIT DVMLYSSDYP ARQEAVGALF GAVESWVQNP 02000201 APLRDGVITG FLAAADGPDK VTEGEVIVWT VGMIITGYET TGSLISASLY 02500251 EALRRPPEER PGTDEEIKSW IEEALRVHPP FPHPTWRFPT EDIELGGYLI 03000301 PKGAPVQVSI AAANRQPGEG ADSFEAARGG HGHLSFGLGM HYCIGASLVR 03500351 LEAQIAVREF LRRFPKARLS DGSAVQWESE WMIRRLSALP AVLN

Alphafold2

244A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP245

>CYP245A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MASATLPRFD LMGWDKKDIA DPYPVYRRYR EAAPVHRTAS GPGKPDTYYV 00500051 FTYDDVVRVL SNRRLGRNAR VASGDTDTAP VPIPTEHRAL RTVVENWLVF 01000101 LDPPHHTELR SLLTTEFSPS IVTGLRPRIA ELASALLDRL RAQRRPDLVE 01500151 GFAAPLPILV ISALLGIPEE DHTWLRANAV ALQEASTTRA RDGRGYARAE 02000201 AASQEFTRYF RREVDRRGGD DRDDLLTLLV RARDTGSPLS VDGIVGTCVH 02500251 LLTAGHETTT NFLAKAVLTL RAHRDVLDEL RTTPESTPAA VEELMRYDPP 03000301 VQAVTRWAYE DIRLGDHDIP RGSRVVALLG SANRDPARFP DPDVLDVHRA 03500351 AERQVGFGLG IHYCLGATLA RAEAEIGLRA LLDGIPALGR GAHEVEYADD 04000401 MVFHGPTRLL LDLPDAA

Alphafold2

245A1_m3.pdb

.fasta

Heme Docked

PDBj

2z3t

CYP245A1 (P450StaP) WT

CYP246

>CYP246A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MESPATQVDP ANSPLEPYHI YPEAKSCPVA KVGLWNGTPA HVFSGYEDVR 00500051 TVLQDRRFSS DSRRPNFTEL TPTLQSQAAA PPFVRTDNPD HRRLRGTIAR 01000101 EFLPKHIELL RPAIREIVQG VLDGLAETAP PQDMLEAFAV PVASATVFRL 01500151 LGIPAEDRAL LTRCVKGVVS AVGSEDEGAE VFRTLGEYIG GLVQDPSELP 02000201 EDSLIRRLVT GPYQEKQLTF HETIGVILML IVGGYDTTAS TISLSLVSYA 02500251 LQPEKFSVVH EHPERIPLLV EELLRYHTVS QLGLGRIATE DVEVGGVTVR 03000301 AGQMVVAALP LANRDESVFP NPDELDFDRP SVPHVGFGYG PHQCVGQALA 03500351 RVELQEAIPA VIRRLPGMRL ACALEDLPFR HDMATYGIHE LPMTW

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

6f0b

CYP246A1 (P450TxtC) WT

CYP247

>CYP247A1 (deleted)---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRARAPLHHQ VLPDGREFWS VTRYDDVCRV LGEHQRFTSE RGTVVTHLGV 00500051 DDVAAGTLLT STDPPRHTLV RRAIGARLTA RAVAPWRERI PERDWDELVQ 01000101 LTAMVTAPSD PHFRHGSEAA TLAIAHHELV TYVKEWAARR RSAGGDDGSL 01500151 LDHLMTVRVA GAPLTDEEIA LDGYSILLGA NVTTPHTVSG TVLALIERPE 02000201 QFGKVQADPS LVPNLVEEGL RWTSAACNFM RYAVDDVRIA GGTIPARGAV 02500251 VAWIGSANRD ESQFADPHTF DVTRNASRQV AFGYGPHYCV GAPLARLTLR 03000301 VFFKELLRRF GSLSSGGS
>CYP247A2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRLSPGAASD VDLDRLDLFD LDLYSSGDPH AIWEVMRAQA PLHHQVLADG 00500051 RAFWSVTRYD DVRAVLGDHR RFTSERGTVV THLGEDDLTA GKLMTSTDPP 01000101 RHTQVRRAIG TRLTARAVAS RQNQIRQAIV RFLEPLLDGG TDDLAERALR 01500151 LPMVVAGPLL GIPERDWDEL VQLTAMVTAP SDPHFQCGGR AATLAIAHHE 02000201 IVTYVTDWVR RRRSSGERDG SLLDHLMSVR AGDAPLTDEE IAYDGYSLLL 02500251 GANVTTPHTI SGTVLALVEL PEQFDKAQAD PTLVPSLVEE GLRWTSAACN 03000301 FMRYAACDTE IGGGTVRRGD AVVAWIASAN RDESRFADPH EFDLTRDANR 03500351 HVAFGVGPHY CIGAPLARLT AQIFFDELLD RCASIELGGA PEHLRSYFIA 04000401 GMTHLPVVAR KRRSS

Alphafold2

247A2_m3.pdb

.fasta

Heme Docked

PDBj

CYP248

>CYP2A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTAFDPTDAD VRRDPYPSYH WLLRHDPVHR GAHRVWYVSR FADVRAVLGD 00500051 ERFARTGIRR FWTDLVGPGL LAEIVGDIIL FQDEPDHGRL RGVVGPAFSP 01000101 SALRRLEPVI AGTVDDLLRP ALARGAMDVV DELAYPLALR AVLGLLGLPA 01500151 ADWGAVGRWS RDVGRTLDRG ASAEDMRRGH AAIAEFADYV ERALARRRRE 02000201 GGEDLLALML DAHDRGLMSR NEIVSTVVTF IFTGHETVAS QVGNAVLSLL 02500251 AHPDQLDLLR RRPDLLAQAV EECLRYDPSV QSNTRQLDVD VELRGRRLRR 03000301 DDVVVVLAGA ANRDPRRYDR PDDFDIERDP VPSMSFGAGM RYCLGSYLAR 03500351 TQLRAAVAAL ARLPGLRLGC ASDALAYQPR TMFRGLASLP IAFTPGG

Alphafold2

248A1_m5.pdb

.fasta

Heme Docked

PDBj

3buj

CYP248A1v (CalO2)

CYP249

>CYP249A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTLSLATAQE RYATDADVFA HDTLVDPYDT YRSLRDIGRV SYMTRYDTWA 00500051 LTRYDEVRHA LGDWQTFSSA QGIGMSTALN EAWKDFAPCK DGADHLPMRK 01000101 LMMQDLGPKA AAAYKEKIQQ AAVTLVEELL DRREFDAVLD FAQMMPMRVF 01500151 MEVLGVEPDI EQRRTMLHWG TDTYNCAAPD GLYDDTLPSM DKLYSWALEN 02000201 ITPETAREGS VAASTWESVE RGDITDVQAV ATLAAYVTAG LDTTAGTLGN 02500251 TIAQFAANPD QWAIVRDDPK TIPGAILEGI RFDSVAQWFT RVTTRDVEYD 03000301 DIVIPAGSRT YHSYAAANRD ERHYRDPDSF DVLRNPTDHV GFGYGPHMCV 03500351 GKSVSNTEMI ALWTELGRRV DRIEQIGPKK QHINNLIRSL DSLPVRIYPK 0400

Alphafold2

249A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP250

>CYP250A1 (C-deleted)---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKEPLDFADP TLYQNPVPAF NKMREEHPVF WSDSAGSWVV SRHADVVRVL 00500051 NNLEDAQASL FKINDYAEQC PFGKGTAISR GIENALVTTD LPDHPRLRRH 01000101 TAPLLTRRSV ERDYAETVEQ TVIALLEGIE EDTRFDVLDS ISVPLPLAVV 01500151 TKLIGFDAED
>CYP250A1v---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKEPLDFADP TLYQNPVPAF NKMREEHPVF WSDSAGSWVV SRHADVVMVL 00500051 NNLEDAQASL FKINDYAEQC PFGKGTAISR GIENALVTTD LPDHPRLRRH 01000101 TAPLLTRRSV ERDYAETVER TVIELFEGIE EDTRFDVLDS ISVPLPLAVV 01500151 TKLIGFEAED PLQLNHWAVD LTRSLEPHLD SDSLEASDKA ATALEDYLQT 02000201 VLRDHASTAP AGTVLQRLSQ GMEEGTLLSE QEAVVRLIEL VAAGTETTTT 02500251 IIPAAFEAMT QFPHVWKELQ ADPSLIPAAA EEFLRFATPS PFTGRVAVKD 03000301 IPLGEHVIRP GDSIQLAILA ANRDPRVFSE PDVLDIHRTP NPHITFGGGI 03500351 HACLGQHLAR LELITVLKHA VSRWESFEVA TEQVVRRDRI GVWGYSAFPM 04000401 TVQMRAA