Microscopy : Histopathology and Histology of Skeletal Muscle

 Skeletal muscle tissue which signs of DMD.

DMD  signs: 

  Muscle fibers

    Size: Varied

    Shape: Small fibers round or polygonal

    Internal nuclei

    Hypercontracted

    Necrosis

  Endomysial connective tissue: Increased between muscle fibers

  Fat: Increased in endomysium & preimysium

1.Compare this to your microscopic images of skeletal muscle.

2. Read  about the normal histology of skeletal muscle and view microscopic samples.  This will help you to identify structures on your microscope slide of skeletal muscle.  ( See the image o this web page, below.)

LAB : Slides : 

1. a. View your slide under low power on the digital microscope.  Practice labeling and measuring.  Save this image in your Google folder. Insert it into a Google drawing. 

       b.Label it with your name. Magnification.  Take a measurement in um. Label what it is. Labels should be made under the image, not on the image.  The exception is the measurement.

Each person should take their own image...They should all be different!

    So, required work :  

                    1. One Low Power Slide image 

                      2.  One High Power Slide image with calibrated measurements, and    labeled parts.

2. View the slide under high power.  Label it with your name and a title.  Make  at least one  measurement of a structure in um.  Attempt to measure the sarcomere. Save this to your Google folder.  Additional labels can be made using Google Draw.

Try to identify structures.  Use arrows and letters or numbers to identify structures.  Then, make a key under the image. This is so structures are not blocked by your labels.

Define and identify on your slide:   Review the images A,B,C,D for help with identifications

1. A-Bands  ( See image D)    NOTE : You may zoom in on an area using your computer to identify the parts (Red Bands)

2. I-Bands  ( White Bands)

3. Sarcomeres - The unit of contraction; a structure from Z to Z-Band ( Z-band is the center of the I-Band and may not be clear)

4. Striations  ( stripes)

5. Label cells as transversely cut  ( a cross section) or longitudinally cut cells.

6. Label your image with the magnification used.

7. Label your image with a title and include your name.

8. Are there any other structures visible?  What might they be?  Label them.

Image A

Image B

Image C

Image D

Slide Share : You may be able to search and access Slides produced by physicians on the topic : SLIDES