Annotation of the Bifidobacterium longum Genome from DNA Coordinates 26169 to 29638 (or Locus Tags BL0021 to BL0024)

Advisor: James Payne IV

Student Researchers: Crystal Lee, Elizabeth Davlin, and Sara Abdel Khalikh Alhamoud

Abstract: A group of consecutive 4 genes from the microorganism Bifidobacterium longum (BL0021 – BL0024) were annotated using the collaborative genome annotation website GENI-ACT. The Genbank proposed gene product name for each gene was assessed in terms of the general genomic information, amino acid sequence-based similarity data, structure-based evidence from the amino acid sequence, cellular localization data, potential alternative open reading frames, and enzymatic function. The Genbank proposed gene product name did not differ significantly from the proposed gene annotation for each of the genes in the group and as such, the genes appear to be correctly annotated by in the r database. The 4 genes studied in this research acts a an operon to produce a quaternary structure ATP-binding cassette (ABC) transporter to translocate glutamate through the cytoplasmic membrane into the bacteria.

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