Annotation of the Photorhabdus luminescens Genome from DNA Coordinates 4488317..4489174 (Locus Tag PLU_RS1965)

Advisor: Lawrence Grisanti

Student Researcher: Leah Stucke

Abstract: A gene from the microorganism Photorhabdus luminescens (PLU_RS18965) was annotated using the collaborative genome annotation website GENI-ACT. The Genbank proposed gene productname for the gene was assessed in terms of the gene sequence, structure, localization, alternate open reading frames, enzymatic function, duplication and degradation, and horizontal gene transfer. Through the use of various online programs the gene was annotated and found to match the proposed product names. The results presented by the softwares used generally did not differ significantly from the proposed gene annotation for the genes in the group and as such, the gene appears to have been correctly annotated by the r database. According to gene annotation websites and software gene PLU_RS18965 was correctly named as a chaperone protein called Photorhabdus luminescens subsp. laumondii TTO1.

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