The Medical Microbiology Laboratory provides Bacteriology and Serology services for state of Melaka. Bacteriology laboratory is particularly involved in isolation or establishing the causative organisms as well as monitoring and screening of diseases. Serology is the scientific study of plasma, serum and other bodily fluids. In practice, the term usually refers to the diagnostic identification of antibodies in the serum. Such antibodies are typically formed in response to an infection (against a given microorganism), against other foreign proteins (in response, for example, to a mismatched blood transfusion), or to one's own proteins (in instances of autoimmune disease).
Head of Unit
Bacteriology laboratory provides the following services:
Diagnostic Microbiological services which comprise of direct microscopic observation of pathogens, culture and sensitivity testing of bacteria and fungus as well as identification of medically important parasites.
Participation in hospital wide infection control activities related to surveillance, control and prevention of nosocomial infections.
Provision of microbiological studies of the hospital environment and sterility testing.
Serology laboratory provides the following services:
Virology, Serology, Immunology and Molecular testing
Participation in national epidemiological activities such as dengue surveillance, Severe Acute Respiratory Infection Surveillance (SARI) and other emerging outbreak diseases such as leptospirosis, Influenza, SARS-CoV-2, MERS-CoV, Zika Virus and etc.
Serological and virological investigations for medicolegal cases.
Tests required to be informed to Scientific officer / Medical Microbiologist before sending specimens:
Organ Transplant Cases
Zika PCR
MERS-CoV PCR
24 Hours (Bacteriology)
Afternoon shift : Monday – Thursday : 2.00 pm – 9.00 pm
Friday : 2.30 pm – 9.00 pm
On call : 9.00 pm – 8.00 am
Saturday – Sunday / Public Holiday : 8.00 am – 8.00 am
Office Hours (Serology)
Weekday : 8.00 am to 5.00 pm
Weekend : 8.00 am to 2.00 pm
PER PAT 301 (1 copy)/ 3 copies (for H. Sg Buloh only)
Permohonan Ujian TB - TBIS 20C
Brucellosis Laboratory Request Form (IMR/IDRC/BACT/BRUCE/03)
Bacteriology Request Form (IMR/BACT/FORMS/SMIS/01 Version 6.0)
Mycology Request Form (IMR/IDRC/BACT/MYCO/01)
Borang Permohonan Ujian Calitan Torehan Kulit Kusta - LIS102A
Borang Permohonan Kusta - LIS102C
Borang Permohonan Ujian Makmal (Spesimen Klinikal) - MKAK-BPU-U01/Rev2018
Borang Permohonan TB Targeted Next Generation Sequencing (NGS) Drug Susceptibility Test (DST) - IMR/IDRC/BACT/TBNGS/01
Additional verbal requests using existing primary specimens shall be followed by new request form with details of requester, date and time of request. Acceptance of this requests are subjected to specimen stability or according to unit criteria.
All serological tests can be requested using PER PAT 301 form except for the following tests.
Additional verbal requests using existing primary specimens shall be followed by new request form with details of requester, date and time of request. Acceptance of this requests are subjected to specimen stability or according to unit criteria.
General Specimen Collection Guidelines
The quality of laboratory results depends greatly on the proper collection and handling of the specimen as well as obtaining satisfactory material for examination.
The clinical specimen must be material from the actual infection site and must be collected with minimum contamination from adjacent tissue, organs or secretions.
A sufficient quantity of specimen must be obtained in order to perform the examination required.
Appropriate collection devices, specimen containers and culture media must be used to ensure optimal recovery of microorganisms.
Ideally, the specimen must be collected before the commencement of antibiotic therapy.
Specimens are best transported immediately to the laboratory.
All culture and sensitivity tests requests are advised to fill up one copy of PER.PAT 301 form.
Specific requirement criteria for each specimen is listed below under Notes in specific type of specimen collection's guide.
For common rejection criteria in Microbiology Laboratory, please refer to the list below.
Method of Blood Collection for General Test:
Draw 3-5 ml of blood into a plain tube with / without gel (without anticoagulants).
Clot at ambient temperature.
Dispatch to the laboratory within 4 hours of collection for serum separation by centrifugation.
For HCV Viral load, request forms must be signed by a specialist / consultant.
Note:
Haemolysed, icteric or lipaemia specimens invalidate certain tests. If such specimens are received, the samples will be rejected to assure that results are of clinical value.
Form and specimen not tally
Insufficient info on demographic / information for testing
Insufficient volume
Leaking
Name and I/C not tally
No specimen/ form received
Sharing form for many tests
Repeating sample between inappropriate time
The blood bottle not sent/collected in pair at the same date and time (CRBSI)
Sample collected in wrong container
Sample not labelled
Test not offered
Unsuitable specimen for testing (salivary specimen)
Unsuitable specimen for testing
AFB smear POSITIVE (sent > 3 samples at diagnosis)
AFB smear POSITIVE (subsequent samples sent within 2 weeks)
BFMP smear (Positive) (sent > 1 time within 24 hours)
BFMP smear (Negative) (sent > 1 time within 12 hours)
Poor BFMP smear (Wet smear)
Poor BFMP smear (Detached smear after staining)
Unsuitable Specimen BFMP (Label sticker on smear)
Unsuitable specimen for testing (Cover the manufacturer's blood bottle sticker)
Unsuitable specimen for testing (Cover blood bottle bottom sensor)
Hard stool for Ova & Cyst / C. difficile/ Rotavirus/ Adenovirus testing
Rotavirus request for children >5years
Wrong labelling
Wrong request form
Wrong test requested
Sending ATTEST (Sterrad) samples more than 2 hours
Unsuitable specimen (Frozen urine)
Unsuitable specimen (Urine collected from urinal/urine bag)
Unsuitable specimen (Foley catheter tip)
Unsuitable specimen (>2 hours urine in sterile container)
Unsuitable specimen (>24 hours urine in boric acid container)
Repeat urine culture within 2 days of a previous urine culture
Inadequate blood bottle volume
Basic Triple Packaging System as illustrated below is designed for the purpose of safe transport of infectious substances and diagnostic specimens. The system consists of three layers as follows:
Primary receptacle
The container (e.g. tube, vial, and bottle) that holds the specimen.
Must be securely sealed and leak-proof (Screw-top tubes must have a piece of waterproof tape around the top to prevent the top from coming loose in transit).
Must be surrounded by absorbent material capable of taking up the entire liquid contents.
Must be packed in the secondary receptacle in such a way that it will not break.
Secondary packaging
The receptacle into which a primary receptacle and the absorbent and cushioning material are placed which is must be leak-proof and securely sealed.
Must be placed in the outer packaging so that it does not move.
The biohazard marking should be on the secondary receptacle and may be on the primary receptacle.
An itemized list of contents is required and is placed OUTSIDE the secondary container. DO NOT place documents inside the secondary container.
The laboratory request form should be placed OUTSIDE the secondary container.
Outer packaging
The receptacle where the secondary packaging with cushioning materials is placed.
The outer packaging must be rigid.
The outer packaging bears the addressing information along with all required markings and labels such as:
The full name and address of the shipper and consignee must be on the outside packaging.
The outside packaging must have the name and telephone number of a person who is knowledgeable about the contents of the shipment.
This is important emergency information in the event an exposure occurs during shipping.
Medical Consultation
06 -289 2862
Result Consultation
06 -289 2860/63
Demographics Query / Addition
06 -289 2879
Demographics Query / Addition
06 -289 2881
06-556 2333/160 (HAG)
06-5294262/212 (HJ)