Cell names and growth conditions: ___________________________________________________
Method of collecting lysates: ________________________________________________________
Volume and total mass of protein loaded: ______________________________________________
Name of ladder: __________________________________________________________________
Type of gel: ____________________________ Type of membrane: ________________________
Voltage, temp, and length of transfer: _________________________________________________
Blocking buffer ingredients & concentration: ____________________ Blocking time: __________
Primary Ab used (include clone, company & catalog #): __________________________________
Primary Ab concentration: ___________________ Time/temp of primary incubation: ___________
Blocking/washing time: ____________________________________________________________
Secondary Ab used (include clone, company & catalog #): _________________________________
Secondary Ab concentration: ________________ Time/temp of secondary incubation: ___________
Blocking/washing time: ____________________________________________________________
Tertiary Ab/reagent used (include clone, company & catalog #): _____________________________
Tertiary Ab/reagent concentration: ______________ Time/temp of tertiary incubation: ___________
Blocking/washing time: ____________________________________________________________
Detection Reagent used (Name, company): _____________________________________________
Amount of detection reagent: ________________ Detection reagent incubation time: ____________
Name/date of image file(s) on proteinsimple machine: _____________________________________