Western Blot Details

Western Blot Details. Initials: ________ Date: _____________

Cell names and growth conditions: ___________________________________________________

Method of collecting lysates: ________________________________________________________

Volume and total mass of protein loaded: ______________________________________________

Name of ladder: __________________________________________________________________

Type of gel: ____________________________ Type of membrane: ________________________

Voltage, temp, and length of transfer: _________________________________________________

Blocking buffer ingredients & concentration: ____________________ Blocking time: __________

Primary Ab used (include clone, company & catalog #): __________________________________

Primary Ab concentration: ___________________ Time/temp of primary incubation: ___________

Blocking/washing time: ____________________________________________________________

Secondary Ab used (include clone, company & catalog #): _________________________________

Secondary Ab concentration: ________________ Time/temp of secondary incubation: ___________

Blocking/washing time: ____________________________________________________________

Tertiary Ab/reagent used (include clone, company & catalog #): _____________________________

Tertiary Ab/reagent concentration: ______________ Time/temp of tertiary incubation: ___________

Blocking/washing time: ____________________________________________________________

Detection Reagent used (Name, company): _____________________________________________

Amount of detection reagent: ________________ Detection reagent incubation time: ____________

Name/date of image file(s) on proteinsimple machine: _____________________________________