CIP treatment

Overview

This protocol describes how to use Calf Intestinal Phosphate (CIP) to remove 5' phosphates from DNA

Protocol

• Suspend DNA in 1X NEB buffer (0.5 μg/10 μl)
• Add 0.5 units of CIP/μg vector DNA
• Incubate 60 minutes at 37°C
• Purify DNA by gel purification, spin-column purification or phenol extraction.