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Candidate Cancer Gene Database
In The News
Ovarian Supergroup
Archived schedules
Ovarian SuperGroup Schedule 2020-2021
Ovarian SuperGroup Schedule 2019-2020
Ovarian SuperGroup Schedule 2018-2019
Ovarian Supergroup for 2017-2018
Ovarian Schedule for 2016-2017 Academic Year
Ovarian Supergroup 2015-2016
Ovarian Supergroup 2014-2015
Ovarian Supergroup 2013-2014
Ovarian Supergroup 2012-2013
Ovarian Supergroup 2011-2012
People
Protocols
Apoptosis Assays
AnnV-PI-7AAD
Hoecsht Apoptosis Assay
Cell Work
Cell_line_authentication
Colony forming cell assay
Counting Cells
Culture Freeze Thaw Cells
Intestinal Organoid Culture
Proliferation MTT/MTS assay
Soft Agar Colony Formation Assay
TKS Batch Count Colonies
Cloning and Transfection
Agar plates
CIP treatment
Cloning transgene integration sites
CRISPR/Cas9
Electroporation
Electroporation using Neon Electroporation from Invitrogen
Gateway Cloning
Klenow Reaction
Preparation and transformation of chemically competent E. Coli using CaCl2
Preparation and using glycerol stocks of bacteria
Tranformatio of ES cells using Electroporation
Transfection using Lipoectamine
Transformation of ES cells using electroporation
DNA
Amplicon sequencing using the Illumina GAIIx platform
cDNA synthesis using SS III from Invitrogen
DNA extraction from cultured cells
DNA extraction from tail clips
DNA restriction digest
Genomic DNA from fresh or frozen tissue
Plasmid purification from E. Coli
Flow cytometry
Microscopy
IHC of paraffin sections
Microtome sectioning
Nikon Eclipse TE2oo microscope
Paraffin embedding tissue
Miscellaneous work
Coating beads with molecules for immune stimulation
Mouse Work
Colonoscopy using Karl Storz Coloview system
Intestinal polyp counting
Xenografts
Nanoparticles
DNA-AuNP Thiol Conjugates
PCR
Gel Electrophoresis
General PCR
Quantitative Real-Time PCR
Roche Light Cycler 96 PCR protocol
Quantitation
Protein quantification using Bradford Assay with Coomassie
Quantification iof DNA using Epoch BioTek plate reader
Quantification of protein bands using densitometry
RNA
cDNA sythesis using Vilo from Invitrogen
DNase treat RNA using Ambion Turbo DNA-Free kit
RNA isolation using Trizol
Single nuclei RNA sequencing
miScript_PCR_System
Western Blot
Chemifluorescent detection of protein on a Western Blot
Making SDS-PAGE Gels
Protein Purification for WB
Separate proteins on an SDS-PAGE gel
Stripping and Reprobing a Western Blot Membrane
Transfer proteins from gel to membrane
Western Blot Details
Western Blot Dot Blots
Western Blot Presentations
Western blot protein detection using chemiluminescence
Single cell RNA sequencing
Publications
Sleeping Beauty DNA transposon
Starr Lab Standard Operating Procedures
Adcre Surgery Supply Checklist
Cell Culture
Chemotherapy
Cloning
DNA and RNA extraction
In Vivo Cell Implantation
In vivo viral gene delivery
Mouse Breeding, weaning, and necropsy
PCR
Protein Extraction and Western Blotting
Surgery Procedure Checklist Template
Transfection
Viral Transduction
StarrLab
Protein Extraction and Western Blotting
170328_SOP_Protein_Extraction_Westerns_TKS.docx
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