Generate cDNA from isolated RNA. Generally, the procedure requires the addition of a short template that will bind to the RNA and then addition of an enzyme that will synthesize DNA using the RNA with the template attached. The template can be oligo(dT) (a string of TTTTTT), which will bind to the polyA tail of mRNA; random hexamers, which will bind anywhere the random sequences lie in the RNA; or specific primers, which are targeted to specific RNA. Currently we are using the Superscript III First-strand synthesis kit from Invitrogen. But also included in this protocol is the older Superscript II First-strand synthesis protocol. Read the Superscript III Platinum two-step qRT-PCR kit with SYBR Green instructions for more info.