Last updated: 1/12/12 By: Tim Starr
You can either buy the Invitrogen NuPage prepoured gels, or pour your own gel using Invitrogen Gel Cassettes (1.0mm cat# NC2010). Or use any other electrophoresis apparatus. The gel we currently use is a Bis-Tris acrylamide gel. Generally I use a 10% acrylamide gel, but you can vary the percentage depending on the size of the protein you are looking for. The recipes below are adapted from Invitrogen.
Separating Gel Recipe for 4 gels (approximately 25 ml)
Stacking Gel Recipe for 4 gels (approximately 12.5 ml)