Last updated: 1/12/12 By: Tim Starr
Follow this protocol to strip off antibodies from a membrane in order to re-stain the membrane with a different antibody. Generally use mild stripping, unless it doesn't work. PVDF membranes are more durable and will withstand more stripping and reprobing. Can check efficiency of stripping by incubating with chemiluminescent reagent and image the blot. This protocol comes from the Abcam protocol
Mild stripping buffer (100 ml)
Harsh stripping buffer (100 ml)