The protocol was adapted from the Puregene Genomic DNA purification kit by Gentra. Generally start with 50 to 100 mg fresh or frozen tissue (about the size of a pencil eraser) and expect to get between 20 to 150 µg DNA. If there is more tissue, or it is possible to scale up and use a dounce homogenizer in the first step instead of using a razor blade to mince the tissue.
Cell lysis buffer 100 ml
Protein Precipitation solution 50 ml