Unit 6 Gene Expression & Regulation

Thursday 3/6/2025

Today's Focus: Restriction Enzyme Digestion Lab

Bell Question

1. No Bell Question, we will immediately get started on the lab

Today's In-class Tasks.

1. We will conduct the Restriction Enzyme Digestion Lab, which uses different different restriction enzymes to cut DNA in specific locations.

   1. 1. • A hard copy of the lab handout.

Assignment(s):

1. 1. Complete the Unity 7 Topics 4, 5 and 12 Lecture Notes. Due Monday 3/10

      1. • The videos are in Google Classroom

         • We ware running labs for the next 3 classes. Unit 7 work will be given but we will not start addressing the concepts until after the labs.

   2. Topics 1 - 3 are covered in Chapter 19 of the book

      1. • Read and take any additional notes needed

           1. • Use the Unit 7 Objectives and Understandings to guide your note-taking

Supportive Media

1. Unit 7 Objectives and Understandings

Tuesday 3/4/2025

Today's Focus: Amplifying DNA with Various Primers and PCR

Bell Question

1. Place your homework on the table in front of my desk and take:

   1. • Tonight's homework, Topics 4, 5 and 12 Lecture Notes

      • A copy of today's Lab

Today's In-class Tasks.

1. We will conduct the PCR 101 Lab, which uses different primer sets to amplify different segments of DNA.

   1. 1. • A hard copy of the lab handout, along with several lab videos is given to you in Google Classroom.

Assignment(s):

1. 1. Complete the Unity 7 Topics 4, 5 and 12 Lecture Notes. Due Monday 3/10

      1. • The videos are in Google Classroom

         • We ware running labs for the next 3 classes. Unit 7 work will be given but we will not start addressing the concepts until after the labs.

   2. Topics 1 - 3 are covered in Chapter 19 of the book

      1. • Read and take any additional notes needed

           1. • Use the Unit 7 Objectives and Understandings to guide your note-taking

Supportive Media

1. Unit 7 Objectives and Understandings

Wednesday 2/28/2025

Today's Focus: Unit 6 Assessment

Bell Question

1. No Bell Question, get into test taking seats.

Today's In-class Tasks.

1. Students will complete the Unit 6 assessmenty

Assignment(s):

1. 1. Complete the Unity 7 Topics 1 - 3 and DDT Case Study. Due Tuesday 3/4

      1. • We ware running labs for the next 3 classes. Unit 7 work will be given but we will not start addressing the concepts until after the labs.

   2. Topics 1 - 3 are covered in Chapter 19 of the book

      1. • Read and take any additional notes needed

           1. • Use the Unit 7 Objectives and Understandings to guide your note-taking

Supportive Media

1. Unit 7 Objectives and Understandings

Wednesday 2/26/2025

Today's Focus: Operons / Epigenetics / PCR / Electrophoresis

Bell Question

1. People who are diabetic have cells that are unable to take in glucose. They lack or are deficient in a hormone that signals their cells to bring the GLUT transport proteins to the cell surface in order to move the glucose into the cells.

   1. • What is the hormone?

      • If they can not produce this hormone in sufficient quantities, they must inject the hormone into their body after meals.

        1. • Where does the injectable hormone come from?

Today's In-class Tasks.

1. We will start the class by reviewing answers to the Trp & Lac Operons Worksheet.

2. We will then discuss epigenetics:

   1. 1. • will use the BioNinja page on Epigenetics in our discussion as well as Image 1.

         • Our discussion on epignetics will continue with Video 3, Amoeba Sisters - Epigenetics

         • We will finish our discussion on epigenetics by discussing microRNAs and siRNAs

3. We will then discuss Gene Manipulation, PCR and Electrophoresis

   1. 1. • We will discuss the videos below.

Assignment(s):

1. 1. Prepare for the Unit 6 assessment, to be given on Friday.

Supportive Media

1. BioNinja link on DNA structure

Monday 2/24/2025

Today's Focus: Micropippeting Practice / Operons / Transcription Factors / Epigenetics

Bell Question

1. Take out your Topics 7 & 8 Guided Lecture Notes and your Cystic Fibrosis Case Study and a Restriction Enzymes assignment, place them in two piles on your tables.

2. While I am checking homework, students will watch Video #1 on Micropipetting 

Today's In-class Tasks.

1. Students will complete a micropipette activity after watching the video on how to use a micropipette.

2. Once finished, students will check their Topics 7 & 8 Guided Lecture notes and the Cystic Fibrosis Case Study and a Restriction Enzymes assignments with the Answer Keys

3. We will review the TRP & Lac Operons by completing this Biology Corner Trp & Lac Operons Worksheet.

4. We will then watch Video 2 on transcription factors and:

   1. 1. • • • Use this BioNinja page on Gene Expression to discuss the function of transcription actors.

             • discuss Image 1, the charge of DNA molecules, methylation, acetylation and epigenetics

               1. • We will use the BioNinja page on Epigenetics in our discussion

         • Our discussion on epignetics will continue with Video 3, Amoeba Sisters - Epigenetics

Assignment(s):

1. 1. Complete the Trp & Lac Operons Review sheet and teh Gel Electrophoresis worksheet, both given in class. Due on Wednesday.

   2. Prepare for a Unit 6 Exam on Friday

      1. • The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book.  Remaining topics are covered in Chapters 14 & 15.

           1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

   3. Add any questions you have to the Unit 6 Questions shared document, given in Google Classroom

      1. • Wednesday is the last day for me to address any questions

Supportive Media

1. BioNinja link on DNA structure

Tuesday 2/11/2025

Today's Focus: Transcription & Translation (continued)

Bell Question

1. Take out your Topics 5 & 6 Guided Lecture and place in one pile on your tables.

2. While I am checking homework, complete the:

   1. •  Prokaryotic vs Eukaryotic Transcription handout and 

      • The Steps in Translation handout

Today's In-class Tasks.

1. Students will check their Topics 5 & 6 Guided Lecture notes with the Answer Key

2. Afterwards, we will go over 1) Prokaryotic vs Eukaryotic Transcription and 2) The Steps in Translation handouts.

3. We will then discuss Topics 5 & 6:

   1. 1. • Students will need to go to the Phet Lac Operon simulator as we will work through the Guided Activity for this simulation. 

         • We will watch the trp Operon video below and respond to the questions on this Biology Corner Trp & Lac Operons Worksheet.

         • We will then watch Video 2 on transcription factors and:

           • • discuss Image 1, the charge of DNA molecules, methylation, acetylation and epigenetics

               1. • We will use the BioNinja page on Epigenetics in our discussion

         • Our discussion on epignetics will continue with Video 3, Amoeba Sisters - Epigenetics

Assignment(s):

1. 1. Complete the Unit 6, Topics 7 & 8 Video Lecture and Guided Lecture Notes.

      1. • Lecture notes template given in class and the video is in Google Classroom

   2. Along with the Topics 7 & 8 Guided Lecture Notes, I gave students a Cystic Fibrosis Case Study and a Restriction Enzymes assignment.

      1. • Both of these are due the first class after break, 2/24.

   3. The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book.  Remaining topics are covered in Chapters 14 & 15.

      1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

Supportive Media

1. BioNinja link on DNA structure

Friday 2/7/2025

Today's Focus: Transcription & Translation (continued)

Bell Question

1. Get your model DNA strand and, using your homework, transfer the RNA codes and amino acids to your model

Today's In-class Tasks.

1. We will finish the Transcription and Translation modeling activity and then discuss the Analysis questions.

2. We will then go through the differences between Eukaryotic and Prokaryotic Transcription

3. We will also take a closer look at tRNA, tRNA activation and Translation

   1. 1. • I will refer to the BioNinja pages on tRNA and Translation as well as a worksheet on Translation

Assignment(s):

1. 1. Complete the Unit 6, Topic 5 Video Lecture and Guided Lecture Notes.

      1. • Lecture notes template given in class and the video is in Google Classroom

   2. The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book

      1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

Supportive Media

1. BioNinja link on DNA structure

Wednesday 2/5/2025

Today's Focus: Transcription & Translation

Bell Question

1. Take out your Topic 4 Guided Lecture Notes

2. While I am collecting notes and passing out your Topic 5 Assessments, discuss with your partners: "What is the central dogma?"

Today's In-class Tasks.

1. We will discuss the Unit 5, Heredity Assessment.

2. If not finished discussing Meselson & Stahl Experiment, we will.

   1. 1. • We will then watch the Bozeman video below, if nor seen already,  and discuss the Meselson & Stahl Experiment

3. We will then discus transcription and translation

   1. 1. • Students will work through a paper transcription and translation activity

         • This BioNinja link on Transcription may be helpful

Assignment(s):

1. 1. Complete the Unit 6, Topic 5 Video Lecture and Guided Lecture Notes.

      1. • Lecture notes template given in class and the video is in Google Classroom

   2. The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book

      1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

Supportive Media

1. BioNinja link on DNA structure

Monday 2/3/2025

Today's Focus: The Meselson Stahl Experiment & DNA Replication

Bell Question

1. Take out the DNA packet "Class Notes" given last Tuesday and your homework, Unit 6, Topic 3 Guided Lecture Notes.

2. While I collect and credit the homework, students respond to the questions on pages 4, 5 & 6.

Today's In-class Tasks.

1. We will discuss DNA Replication and work through pages 3 - 6 of the Class Notes

2. We will then watch the Bozeman video below and discuss the Meselson & Stahl Experiment

   1. 1. • Afterwards we will discuss and complete the DNA Replication: An Overview handout, part of the Class Notes

           • • This BioNinja link on DNA structure may be helpful.

               1. • This is an IB site and not all information are AP Objectives

3. We will then discus transcription

   1. 1. • Students will work out of the new class notes on transcription

         • This BioNinja link on Transcription may be helpful

Assignment(s):

1. 1. Complete the Unit 6, Topic 4 Video Lecture and Guided Lecture Notes.

      1. • Lecture notes template given in class and the video is in Google Classroom

   2. The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book

      1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

Supportive Media

Thursday 1/30/2025

Today's Focus: The Meselson Stahl Experiment & DNA Replication

Bell Question

1. Open the History and Structure of DNA lecture notes

2. Take out the DNA packet "Class Notes" given in Tuesday

Today's In-class Tasks.

1. We will wrap up the History and Structure of DNA lecture.

2. We will then watch the Bozeman video below and discuss the Meselson & Stahl Experiment

   1. 1. • Afterwards we will discuss and complete the DNA Replication: An Overview handout given in class on Tuesday

3. We will then discuss topoisomerase and watch the Amoeba Sisters video below

   1. 1. • While watching the video we will stop it often and complete the DNA Replication: Key Players handout, given in class on Tuesday

4. Students will then complete the Lagging Strand: A Closer Look handout, given in class on Tuesday, with partners.

   1. 1. • We will discuss the answers when finished.

Assignment(s):

1. 1. Complete the Unit 6, Topic 3 Video Lecture and Guided Lecture Notes.

      1. • Lecutre notes template given in class and the video is in Google Classroom

   2. The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book

      1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

Supportive Media

Tuesday 1/28/2025

Today's Focus: The Pursuit of the Structure of DNA / DNA Replication

Bell Question

1. Take out your Unit 6 Topics 1 & 2 Guided Lecture Notes

2. While scoring your homework, students will work on the Case Study: Bacterial Transformation handout

Today's In-class Tasks.

1. We will discuss the scientists and experiments that shaped our understanding of the structure of DNA.

   1. 1. • I am including here my History and Structure of DNA lecture notes

         • Students should respond to the questions on the Case Study when we are discussing Griffith's experiment.

         • While talking about DNA Structure, students will do a DNA Building Activity

2. With any block time remaining, we will discuss DNA replication

   1. 1. • We will start with the Meselson and Stahl Experiment

         • Students will use the DNA Replication: An Overview handout as guided notes.

Assignment(s):

1. 1. Complete the Unit 6, Topic 3 Video Lecture and Guided Lecture Notes.

      1. • Lecutre notes template given in class and the video is in Google Classroom

   2. The Understandings for Unit 6, Topics 1 - 3 are covered in Chapter 13 of your book

      1. • Use the Unit 6 Learning Objective and Understandings to help you with note-taking

Supportive Media