Acinetobacter baumannii

Introduction

Acinetobacter baumannii is a round rod shaped gram negative bacteria. It was named after Paul Bauman (a biologist). It is a pathogen that affects humans with weakened immune system. It is often linked with hospitals.

Growing Microbe

A. Baumannii is a BSL level 2. Meaning it occurs naturally and is moderately hazardous. These are used to grow the microbe.

Blood Agar

MacConkey agar

Tryptic soy agar

Tryptic soy broth

Visualizing A. Baumannii

The images of Acinetobacter Baumannii through different microscopes.

Gram stain - Negative

Bacteria Morphology

A. aumannii through SEM

A. Baumannii through TEM

A. baumannii vs E. coli through confocal view

Differentiation of Microbe

Differentiation of A. Baumannii

Beta hemolysis

Neutral PH, negative

Positive Dnase

Negative gram staining

Indole positive

Catalase positive

Oxidize negative

Background and Treatment

Acinetobacter baumannii is a nosocomial opportunistic pathogen (contracted by toxins in a certain location) . It causes Acinetobacter baumannii infection. It causes infections in blood, brain, and lungs, it also causes urinary tract and wound infections. It does not have host but is commonly found in water and soil.

It can be prevented though environmental cleaning and proper hygiene. It can be dangerous

There are some antibiotics that can help with it. The antibiotics are: colistin, tigecycline, imipenem and meropenem.

Molecular Diagnosis

AGA AAG GAG GTG ATC CAG CC- Forward

GGC TGG ATC ACC TCC TTT CT- Reverse

Melting temp: 53.8 (basic), 60.5 (salt adjusted), and 53.1 (Nearest Neighbor)

GC content: 55%

16rs primer

AGAAAGGAGGTGATCCAGCC

GGCTGGATCACCTCCTTTCT

About the Researcher

Christina Joseph

Third year Microbiology student on a pre med track

UF Graduate 2022

Love puppies and music and SELFIES

Refrence

Acinetobacter baumannii Bouvet and Grimont (ATCC® 17978™). (n.d.). Retrieved September 23, 2020, from https://www.atcc.org/products/all/17978.aspx

Acinetobacter baumannii. (n.d.). Retrieved September 23, 2020, from https://microbewiki.kenyon.edu/index.php/Acinetobacter_baumannii

https://www.researchgate.net/figure/Confocal-microscopy-experiments-performed-on-E-coli-K12-MG1655-and-A-baumannii_fig3_334660941

https://www.researchgate.net/figure/Scanning-electron-microscopy-of-Acinetobacter-baumannii-ATCC-17978-Cells-were-grown-in_fig3_237069168

https://www.researchgate.net/figure/Transmission-electron-microscopy-images-of-A-baumannii-ATCC-19606-and-the-lptDKm-r_fig5_287109721

http://microbe-canvas.com/Bacteria.php?p=12

Acinetobacter Baumannii. Retrieved October 14, 2020, from https://www.sciencedirect.com/topics/medicine-and-dentistry/acinetobacter-baumannii

Acinetobacter in Healthcare Settings. (2019, November 13). Retrieved October 14, 2020, from https://www.cdc.gov/hai/organisms/acinetobacter.html

Retrieved October 14, 2020, from https://www.uptodate.com/contents/acinetobacter-infection-treatment-and-prevention/print