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LIVE BLOOD ANALYSIS HEMAVIEW WITH DIGITAL DARK FIELD OR BRIGHT FIELD MICROSCOPE
You may not think of your mobile phone as being anything like a microscope, but it has almost all the parts you need. The lens and camera sensor are arranged exactly as they would be inside a microscope – all you need to do to get some magnification is stick another lens in front.
The next part is to think about how you are going to illuminate your sample, which is often just as important as the lenses you use.
There’s been a lot of great work over the past decade or so engineering mobile phone microscopes with amazing capabilities – for example, the Fletcher lab at UC Berkeley, and the Ozcan lab at UCLA – and a lot of it has to do with custom illumination.
The engineering involved to assemble these mobile phone microscopes is not trivial, however. You often need a decent amount of skill and a lab to be able to put these devices together. We wanted to see how simple we could make a microscope, meaning the fewest extra parts and assembly steps possible.
We figured that it made a lot of sense to use the internal flash in the camera to light up your sample. The challenge is that the flash points in the wrong direction – you need to turn it around to shine through the sample and into the camera.
Redirecting light like this usually requires something fancy like a mirror or a prism. But we realised that the flash on a phone is so bright we can just use the diffuse reflection (glare) off regular plastic. So we designed the clip to have a series of tunnels that confine light and turn it
around to face the sample and camera.
Flash illumination is indicated by the blue arrow. Upon striking the illumination backstop (made of the same 3D printed resin as the rest of the clip), this light is reflected diffusely towards the sample and then through the lens into the camera. Right: Cutaway 3D model of the clip-on device, showing the illumination tunnels. Scientific Reports
A lot of light is absorbed by the 3D printed resin of the clip, which is black. But it’s not perfectly black, and even the tiny fraction of light that makes it through the tunnels and reflects off of the black surface is more than enough to light up a microscopic sample. And that’s it – no mirrors, prisms or illumination lenses are needed.
Light and dark This type of illumination is called bright-field microscopy. But we actually went a bit further, and showed that you can turn the flash off and use the Sun to perform dark-field microscopy - where the specimen is lit up, but the field around it is dark.
The clip is designed in such a way that sunlight (or ambient room light) gets trapped in the glass sample slide, and can only be redirected into the mobile phone camera if it hits an object in the sample. If the sample slide is empty, the background is dark (hence dark-field). If there is an object it shines bright on the dark background, and as such this is a great way to detect really subtle objects such as cells (which are mostly water) sitting in water.
We’ve released the design online so that anyone can print it and modify it to suit their needs. This part is important because the mission of low-cost microscopy is to ease access to this high tech equipment. This is best accomplished when everyone has the opportunity to make one for themselves or to adapt it freely.
The clip can be printed using any 3D printer - we prefer the Formlabs family of printers - and you’ll need black resin. The cost in resin per clip is typically a couple of dollars at most. You’ll also need a lens to put in the clip. We buy ours from an online retailer and then remove the lens from the camera module.
Rouleau
RBC Aggregation
Protein Linkage
poor protein metabolism and altered pH or acid imbalance, which varies the electrical negative charge of the cell membrane causing them to stick together.
Macrocytes, Microcytes
The loss of the negative surface charge; this is a more disorganizing symptom where plasma acids act as molecular glue, causing RBCs to stick together
Echinocytes
Over acidity and blood pH imbalance. The diet is high in strong acids from proteins and carbohydrates
Fibrous Thallus
Ingestion of an excessive amount of over acidic food and drinks which causes a deficiency of sodium bicarbonate in the alkalophile glands
Fibrin Spicules
Latent tissue acidosis in the extra cellular fluid and the body’s inability to remove acid waste build up in the blood causing the cells to break down
Mold
Indicates a nationalization of bacteria, yeast/fungus, mold, and their acid wastes and acid crystals lying in a dormant/inactive state
Target Cells
Involved in clotting to prevent internal bleeding. There is usually an increase during detoxification with the complete program and effective diet because the body is pulling acids stored in the connective tissues back into bloodstream for elimination.
Yeast overgrowth, collection of yeast, bacterial, fungus, mold. very toxic. Indicated in advanced stages of latent tissue acidosis. Highly disruptive to normal blood circulation
Fermenting RBCs; White spots or white yeast forms inside RBCs; Indicates the diet is too high in carbohydrates/simple sugars; sugar intolerance and/or imbalance; endocrine system/ pancreas stress
Born out of RBCs due to blood pH Imbalance from latent tissue acidosis; diet too high in protein, carbohydrates/ sugars; may be caused by excess antibiotic use, hormonal therapy, steroid use; fungal outfection
Yeast
Platelets
Rod Forms
Born out of RBCs due to blood pH Imbalance from latent tissue acidosis; diet too high in protein, carbohydrates/ sugars; may be caused by excess antibiotic use, hormonal therapy, steroid use; fungal outfection
Anesthetized WBC's
High counts are due to latent tissue acidosis and excess acids in the bloodstream not being eliminated through the urinary tract causing RBCs to biologically transform giving birth to “filthy, dirty, platelets”.
Basophiles
Bacterial forms born out of RBCs and found in the blood when there is latent tissue acidosis which alters the blood pH; due to acidic diet, emotional or physical stress, low nascent oxygen (O1); waste products of bacteria and yeast/ fungus .
T-cell
Indicates recent consumption of excess sugars/ carbohydrates or proteins; WBCs are paralyzed by the acids (acetyl aldehyde, ethanol alcohol, lactic, nitric, uric, sulfuric, and phosphoric) for up to 5 hours.
Perceived to be related to allergies and/or sensitivities to foods or the environment; exotoxic and mycotoxic reactions; histaminea.
Alergic reactions to dairy.
WBCs that neutralize acids by releasing electrons or “oxygen buffering species” also known as “free radicals” into the blood or lymph plasma; elevated from serious illness, lymphatic stress, environmental chemicals/toxins, drugs or medications,
Crystals
Black Crystals
Cholesterol
Crystals are observed when there is excess acidity. It is the body’s preservation mechanism to buffer acidity and create a solid form which is less toxic than the liquid acids. Crystal are perceived to be he signature of the microzyma fermenting sugar, protein or fat.
Tobacco, marijuana; chemical, recreational and prescription drugs. Brown is also associate with the fermentation of protein.
Usualy indicates high blood pressure, arterial sclerosis, high cholesterol. Diet is too high in animal source proteins. Dehydration, acidosis.
Healthy Unhealthy
Heavy Metals
Low Alkaline Buffers
Circulatory Imbalances
Appears as a double coastline. Perceived to be low alkalophile buffers including sodium bicarbonate and minerals; and spiritual disconnection.
Bowel Toxicity
Appear as one or more raised or rubbed out lighter areas or a blisters if found in 4th Ring. Perceived to be circulatory imbalances, high or low blood pressure, high cholesterol or magnesium deficiency.
Parasites
Perceived to be holding metals in the tissue which may be due to dental fillings, first or second hand cigarette smoke, environmental pollutants, cleaning products, personal care products, water pipes, table salt, acid music, acid thoughts, etc
Reproductive Organs
Stress
Perceived to be small and large bowel holding toxins, possible damage to the intestinal villas, possible pockets in intestine, poor or irregular elimination, and poor digestion with gas, pain or bloating.
Possible sources of parasites include diet, raw fish, improperly cooked protein, pets, travel out of country, water supply, weakened white blood cells. Parasites can only exist in an acidic environment with weakened tissue.
Perceived to be challenges in men to the prostate; in women the uterus, cervix or ovaries; also the bladder; includes emotional imbalances
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