X1. Igem

https://www.genscript.com/express-cloning-vector-list.html

https://genocad.com

https://parts.synbiochem.co.uk

Partes

http://vm-vpr.bioswarm.net:8081/parts

Bacillus

Partes para Bacillus

http://parts.igem.org/Bacillus_subtilis#Plasmid_backbones

Partes de Bacillus subtilis (gram positivo)

http://parts.igem.org/Bacillus_subtilis

E.coli

Promotores

http://parts.igem.org/Promoters/Catalog

Promotores evaluados por Prof. Anderson

http://parts.igem.org/Promoters/Catalog/Anderson

RBS

http://parts.igem.org/Part:BBa_B0034

RBS evaluados por Prof. Anderson

http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Anderson

Ensamblaje de dos piezas

http://parts.igem.org/Help:Assembly/3A_Assembly

Terminadores

http://parts.igem.org/Terminators/Catalog

Doble terminador

http://parts.igem.org/Part:BBa_B0015

Qué es un terminador?

http://blog.addgene.org/plasmids-101-terminators-and-polya-signals

Proteínas fluorescentes

https://www.addgene.org/fluorescent-proteins/plasmid-backbones/

Proteínas reporteras (color)

http://parts.igem.org/Protein_coding_sequences/Reporters

Promotor que se activa con tetraciclina

http://parts.igem.org/Part:BBa_R0040

Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.

Proteína reguladora del promotor de tetraciclina

http://parts.igem.org/Part:BBa_C0040

Proyectos interesantes

Olor http://parts.igem.org/Part:BBa_K1403009

Sabor fruta milagrosa http://parts.igem.org/Part:BBa_K382021

Sabor a manzana http://parts.igem.org/Part:BBa_K395602:Design

Limoneno http://parts.igem.org/Part:BBa_K801061

LEVADURA

Promotor constitutivo de Levadura

http://partsregistry.org/Part:BBa_K517001

Colores para levadura

http://www.addgene.org/browse/article/7252/

Otras secuencias para levadura

http://www.addgene.org/yeast-gateway/#protocols-and-resources

Partes de Plantas

http://parts.igem.org/Collections/Plants

- Arsénico

http://parts.igem.org/wiki/index.php?title=Part:BBa_K957000

- Si se va a utilizar un ensamblaje por bloques, debe recordar añadir los prefijos y sufijos

Para añadir piezas adelante (en el prefijo EcoRI-XbaI)

- La secuencia a insertar se corta con EcoRI- SpeI

- El plásmido o sitio donde se ingresa se corta en EcoRI-XbaI

• (XbaI y SpeI se unen de manera complementaria)

Para añadir piezas atrás (en el sufijo SpeI-PstI)

- La secuencia a insertar se corta con XbaI- PstI

- El plásmido o sitio donde se ingresa se corta en SpeI- PstI

• (XbaI y SpeI se unen de manera complementaria)

Estos son los prefijos y Sufijos que bordean la secuencia que queremos insertar

Protein Coding Region Prefix*

GAATTC GCGGCCGC T TCTAG[ATG Remaining CDS]

EcoRI NotI XbaI

Protein Coding Region Suffix

[CDS]T ACTAGT A GCGGCCG CTGCAG

SpeI NotI PstI

Este es el prefijo para otras partes que no son CDS

• 5' GAATTC GCGGCCGC TTCTAGAG '3 - for all other parts

EcoRI NotI XbaI

Más detalle en http://parts.igem.org/Help:Prefix-Suffix

Un ejemplo es este CDS que está en color ROJO y al cual le añadimos el prefijo GAATTCGCGGCCGCTTCTAG y el sufijo TACTAGTAGCGGCCGCTGCAG

EcoRI

GAATTCGCGGCCGCTTCTAGATGGGACATGTTACATCTCCCTCTAAACGCTATCCTGCCTCTTTTAAGCGGGCAGGTAGTATTCTTGGCGTGAGTATTGCGCTTGCTGCATTTTCCAATGTGGCAGCAGCGGGTTGTGAGTATGTGGT TACCAATAGCTGGGGGTCGGGATTTACCGCAGCAATTCGTATTACTAACTCAACATCTTCTGTAATCAATGGTTGGAACGTCAGCTGGCAATATAACAGCAACCGTGTTACCAACTTATGGAATCCCAACCTTTCCGGTAGCAATCCCTATACGGCGTCCAA CCTGAGTTGGAATGGCACTATTCAACCTGGGCAAACGGTAGAGTTTGGTTTCCAGGGGGTTACCAATAGCGGCACTGTCGAGAGTCCAACAGTGAATGGTGCTGCATGTACTGGCGGAACCAGTTCTTCGGTGAGTTCTTCCAGTGTTGTGAGTTCAAGCTC GTCATCGCGTAGCAGTGTGTCTTCAAGCTCTGTTGTGTCCTCAAGCTCCAGCGTAGTGAGTTCATCGTCTTCCTCTGTGGTCAGCGGCGGCGGCCAGTGTAATTGGTATGGAACCCTGTATCCGTTGTGTGTGAGCACAACCTCTGGTTGGGGTTACGAAAA CAACAGAAGCTGTATCTCTCCATCAACCTGTTCGGCCCAGCCGGCGCCTTATGGGATTGTCGGGGGCTCGAGCTCGCCCAGCTCAATTTCCAGTTCAAGCGTCCGCTCCAGCAGCTCGTCTTCTGTAGTACCGCCTAGCAGTAGCTCATCTTCCAGTGTTCC ATCAAGCAGCTGTTCCAGTGTTAGCTCCAGCTCGGTTGTCTCTTCCAGTTCATCTTCTGTGAGTGTGCCCGGAACCGGTGTGTTCCGTGTTAATACTAAGGGTAACCTGACGAAAGACGGTCAACTGCTGCCTGCGCGTTGCGGGAACTGGTTTGGTTTGGA GGGGCGTCATGAGCCATCAAATGATGCCGATAACCCCAGCGGTGCGCCAATGGAGTTGTATGCGGGTAACATGTGGTGGGTGAATAACAGCCAAGGTTCCGGTCGCACCATTCAGCAGACTATGACTGAGTTGAAGCAGCAGGGTATTACTATGTTGCGTCT GCCAATTGCACCGCAAACCCTGGATGCAAATGACCCGCAAGGTCGCAGTCCAAACCTCAAAAACCATCAATCCATTCGTCAATCCAACGCGCGTCAAGCATTGGAGGATTTCATCAAACTGGCTGATCAAAATGACATCCAGATCTTTATTGATATCCACTC CTGCTCTAATTACGTTGGTTGGCGGGCCGGTCGTTTGGATGCCCGTCCGCCCTATGTGGATGCGAATCGCGTTGGTTACGACTTCACTCGTGAAGAGTATTCCTGTTCTGCTACCAATAACCCCAGTTCTGTTACCAGGTTCCATGCTTACGATAAGCAGAAG TGGTTGGCAAACCTGCGTGAAATCGCCGGACTATCCGCCAAGCTGGGGGTAAGTAACCTGATTGGTATTGATGTCTTCAATGAGCCTTATGATTACACTTGGGCAGAATGGCAGGGTATGGTTGAAGAGGCCTATCAGGCGATCAATGAAGTTAACCCCAATA TGCTTATTATCGTTGAAGGTATTTCCGCCAATGCTAATACGCAAGATGGAACACCTGACACATCCGTACCTGTGCCACACGGTAGCACCGACTTGAATCCAAACTGGGGTGAAAACCTCTACGAAGCGGGTGCTAACCCACCCAACATTCCCAAGGATCGCCT GTTGTTCTCTCCACACACTTATGGTCCGTCCGTGTTTGTTCAAAGACAATTCATGGAACCGGCGCAGACAGAGTGTGCAGGGCTGGAAGGTGATGAAGCAGCTCAGGCCAGGTGCGGTATTGTGATTAATCCGACCGTGCTTGAGCAAGGTTGGGAAGAGCAC TTTGGCTATCTGCGTGAATTGGGTTACGGTATTTTGATTGGTGAATTTGGCGGTAATATGGATTGGCCTGGTGCCAAGTCGAGCCAGGCTGACCGTAATGCCTGGAGCCATATCACCACCAACGTTGACCAGCAATGGCAACAGGCGGCGGCAAGCTATTTCA AGAGGAAAGGGATAAATGCTTGCTACTGGTCGATGAACCCTGAATCAGCAGATACCATGGGTTGGTATTTAACTCCCTGGGATTCAGTGACTGCCAACGATATGTGGGGTCAGTGGACAGGTTTCGATCCTCGTAAAACCCAGCTGTTGCACAATATGTGGGG TTTGTAATACTAGTAGCGGCCGCTGCAG

SpeI

Si lo tuviésemos que clonar en un plásmido que se corta con EcoRi y XbaI

>pSB1C3 Part-only sequence (2070 bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctc ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa aaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggt cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacctt cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat atatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacag gagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgcca gcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaga cgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatg aaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgt gaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctt tacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattat ggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaa aaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

EcoRI XbaI

Entonces eliminamos el fragmento interno y los sustituimos por nuestro inserto

>pSB1C3 Part-only sequence (2070 bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctc ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa aaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggt cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacctt cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat atatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacag gagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgcca gcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaga cgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatg aaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgt gaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctt tacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattat ggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaa aaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

EcoRI XbaI

>pSB1C3 Part-only sequence (2070 bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctc ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa aaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggt cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacctt cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat atatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacag gagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgcca gcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaga cgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatg aaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgt gaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctt tacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattat ggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaa aaatccttagctttcgctaaggatgatttctggaaTTCGCGGCCGCTTCTAGATGGGACATGTTACATCTCCCTCTAAACGCTATCCTGCCTCTTTTAAGCGGGCAGGTAGTATTCTTGGCGTGAGTATTGCGCTTGCTG CATTTTCCAATGTGGCAGCAGCGGGTTGTGAGTATGTGGTTACCAATAGCTGGGGGTCGGGATTTACCGCAGCAATTCGTATTACTAACTCAACATCTTCTGTAATCAATGGTTGGAACGTCAGCTGGCAATATAACAGCAACCGTGTTACCAACTTATGGA ATCCCAACCTTTCCGGTAGCAATCCCTATACGGCGTCCAACCTGAGTTGGAATGGCACTATTCAACCTGGGCAAACGGTAGAGTTTGGTTTCCAGGGGGTTACCAATAGCGGCACTGTCGAGAGTCCAACAGTGAATGGTGCTGCATGTACTGGCGGAACCA GTTCTTCGGTGAGTTCTTCCAGTGTTGTGAGTTCAAGCTCGTCATCGCGTAGCAGTGTGTCTTCAAGCTCTGTTGTGTCCTCAAGCTCCAGCGTAGTGAGTTCATCGTCTTCCTCTGTGGTCAGCGGCGGCGGCCAGTGTAATTGGTATGGAACCCTGTATC CGTTGTGTGTGAGCACAACCTCTGGTTGGGGTTACGAAAACAACAGAAGCTGTATCTCTCCATCAACCTGTTCGGCCCAGCCGGCGCCTTATGGGATTGTCGGGGGCTCGAGCTCGCCCAGCTCAATTTCCAGTTCAAGCGTCCGCTCCAGCAGCTCGTCTT CTGTAGTACCGCCTAGCAGTAGCTCATCTTCCAGTGTTCCATCAAGCAGCTGTTCCAGTGTTAGCTCCAGCTCGGTTGTCTCTTCCAGTTCATCTTCTGTGAGTGTGCCCGGAACCGGTGTGTTCCGTGTTAATACTAAGGGTAACCTGACGAAAGACGGTC AACTGCTGCCTGCGCGTTGCGGGAACTGGTTTGGTTTGGAGGGGCGTCATGAGCCATCAAATGATGCCGATAACCCCAGCGGTGCGCCAATGGAGTTGTATGCGGGTAACATGTGGTGGGTGAATAACAGCCAAGGTTCCGGTCGCACCATTCAGCAGACTA TGACTGAGTTGAAGCAGCAGGGTATTACTATGTTGCGTCTGCCAATTGCACCGCAAACCCTGGATGCAAATGACCCGCAAGGTCGCAGTCCAAACCTCAAAAACCATCAATCCATTCGTCAATCCAACGCGCGTCAAGCATTGGAGGATTTCATCAAACTGG CTGATCAAAATGACATCCAGATCTTTATTGATATCCACTCCTGCTCTAATTACGTTGGTTGGCGGGCCGGTCGTTTGGATGCCCGTCCGCCCTATGTGGATGCGAATCGCGTTGGTTACGACTTCACTCGTGAAGAGTATTCCTGTTCTGCTACCAATAACC CCAGTTCTGTTACCAGGTTCCATGCTTACGATAAGCAGAAGTGGTTGGCAAACCTGCGTGAAATCGCCGGACTATCCGCCAAGCTGGGGGTAAGTAACCTGATTGGTATTGATGTCTTCAATGAGCCTTATGATTACACTTGGGCAGAATGGCAGGGTATGG TTGAAGAGGCCTATCAGGCGATCAATGAAGTTAACCCCAATATGCTTATTATCGTTGAAGGTATTTCCGCCAATGCTAATACGCAAGATGGAACACCTGACACATCCGTACCTGTGCCACACGGTAGCACCGACTTGAATCCAAACTGGGGTGAAAACCTCT ACGAAGCGGGTGCTAACCCACCCAACATTCCCAAGGATCGCCTGTTGTTCTCTCCACACACTTATGGTCCGTCCGTGTTTGTTCAAAGACAATTCATGGAACCGGCGCAGACAGAGTGTGCAGGGCTGGAAGGTGATGAAGCAGCTCAGGCCAGGTGCGGTA TTGTGATTAATCCGACCGTGCTTGAGCAAGGTTGGGAAGAGCACTTTGGCTATCTGCGTGAATTGGGTTACGGTATTTTGATTGGTGAATTTGGCGGTAATATGGATTGGCCTGGTGCCAAGTCGAGCCAGGCTGACCGTAATGCCTGGAGCCATATCACCA CCAACGTTGACCAGCAATGGCAACAGGCGGCGGCAAGCTATTTCAAGAGGAAAGGGATAAATGCTTGCTACTGGTCGATGAACCCTGAATCAGCAGATACCATGGGTTGGTATTTAACTCCCTGGGATTCAGTGACTGCCAACGATATGTGGGGTCAGTGGA CAGGTTTCGATCCTCGTAAAACCCAGCTGTTGCACAATATGTGGGGTTTGTAATActagag

Ejemplo

Promotor regulado por LacI

>BBa_R0010 Part-only sequence (200 bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaat

gtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca

RBS

>BBa_B0034 Part-only sequence (12 bp)

aaagaggagaaa

Red flurorescens protein

>BBa_E1010 Part-only sequence (706 bp)

atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgt

ccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttac

gttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacc

caggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaa

gcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacc

tacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgt

gctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc

Doble terminador

>BBa_B0015 Part-only sequence (129 bp)

ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg

tgggcctttctgcgtttata

Scar

Las partes upstream y downstream se unen en los sitios generados por los cortes de XbaI y SpeI, los cuales son complementarios y generan un SCAR (TACTAGAG). El TA proviene de Spe(TACTAGT)y CTAG de XbaI (TCTAG)y AG es el residuo que queda del prefijo para secuencias no codificantes GAATTCGCGGCCGCTTCTAGAG

Scar para secuencias codificantes

Las partes upstream y downstream se unen en los sitios generados por los cortes de XbaI y SpeI, los cuales son complementarios y generan un SCAR (TACTAG). El TA proviene de Spe(TACTAGT)y CTAG

>BBa_J04450 Part-only sequence (1069 bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagt

tagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa

tactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggt

cgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttac

gttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag

gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttcc

accgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggct

aaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgt

cactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct

gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

También se pueden añadir cambios como por ejemplo un sitio de restricción como NdeI

CATATG

>BBa_K1041000 Part-onlysequence(bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagtt

agctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa

taccatatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag

gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc

ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtt

acccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga

agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacct

acatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct

gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt

cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata