A.Breast Cancer
https://www.cell.com/cms/attachment/1088302/8034710/mmc1.pdf?code=cell-site
PRIMERS
TP53-fwd
TGT GAA GCA GCC ATT CTT TTC
TP53-Rev
CCC ATT TAC TTT GCA CAT CTC A
BRCA1F
GTG ATG AAA ACA TTC AAG CAG AAC TAG
BRCA1R
GGG ATA CAT ACT ACT GAA TGC AAA GGA
BrcaII-Fwd
AAC AGG AGA AGG GGT GAC TG
BrcaII-Rev
GCA CAT CCT GCA CAT GTA CC
PTEN-proF
GGT GCG TCC CAC TCA CAG
PTEN-proR
ATG GGG AGA AGA CGA ATA ATC C
PTEN-exF
TTG GTG TAT GCC TTC ACA TAT CTC T
PTEN-exR
GAA CAA GAT CTG AAG CTC TAC TGG A
CHEK-Fwd
GTG CCT GGC CATGTG GTT AT
CHEK-Rev
GGT GAC AGT GCAAGA CTC CG
27f
AGA GTT TGA TCC TGG CTC AG
1492r
TAC GGY TAC CTT GTT ACG ACT T
ITS1 (Ribosomales para Levaduras)
TCCGTAGGTGAACCTGCGG
ITS4 (Reverse)
TCCTCCGCTTATTGATATGC
40 cycles 95°C 30 s/52°C 1 min/72°C 1 min
SNPs asociados a Cáncer
http://snpedia.com/index.php/Breast_cancer
SNPs importantes
http://www.eupedia.com/genetics/cancer_related_snp.shtml
Sitios SNPS
SNPs con Genes
http://egp.gs.washington.edu/finished_genes.html
Ejemplo Click en Mapping Data
Plant rbcL gene
- rbcLa f 5’- ATGTCACCACAAACAGAGACTAAAGC-3’ (forward primer)
- rbcLa rev 5’- GTAAAATCAAGTCCACCRCG-3’ (reverse primer)
Vertebrate (non-fish) COI gene
- VF1_t1 5'-TCTCAACCAACCACAAAGACATTGG-3' (forward primer)
- VR1d_t1 5'-TAGACTTCTGGGTGGCCRAARAAYCA-3' (reverse primer)
Fish COI gene
- VF2_t1 5'-CAACCAACCACAAAGACATTGGCAC-3' (forward primer)
- FishR2_t15'-ACTTCAGGGTGACCGAAGAATCAGAA-3' (reverse primer )
Fungi ITS
- ITS1 F 5’-TCCGTAGGTGAACCTGCGG-3’ (forward primer)
- ITS4 R 5’-TCCTCCGCTTATTGATATGC-3’ (reverse primer)
Invertebrate COI gene
- LCO1490_F 5’-GGTCAACAAATCATAAAGATATTGG-3’ (forward primer)
- HC02198_R 5’-TAAACTTCAGGGTGACCAAAAAATCA-3’ (reverse primer)