A.Breast Cancer

https://www.cell.com/cms/attachment/1088302/8034710/mmc1.pdf?code=cell-site

PRIMERS

TP53-fwd

TGT GAA GCA GCC ATT CTT TTC

TP53-Rev

CCC ATT TAC TTT GCA CAT CTC A

BRCA1F

GTG ATG AAA ACA TTC AAG CAG AAC TAG

BRCA1R

GGG ATA CAT ACT ACT GAA TGC AAA GGA

BrcaII-Fwd

AAC AGG AGA AGG GGT GAC TG

BrcaII-Rev

GCA CAT CCT GCA CAT GTA CC

PTEN-proF

GGT GCG TCC CAC TCA CAG

PTEN-proR

ATG GGG AGA AGA CGA ATA ATC C

PTEN-exF

TTG GTG TAT GCC TTC ACA TAT CTC T

PTEN-exR

GAA CAA GAT CTG AAG CTC TAC TGG A

CHEK-Fwd

GTG CCT GGC CATGTG GTT AT

CHEK-Rev

GGT GAC AGT GCAAGA CTC CG

27f

AGA GTT TGA TCC TGG CTC AG

1492r

TAC GGY TAC CTT GTT ACG ACT T

ITS1 (Ribosomales para Levaduras)

TCCGTAGGTGAACCTGCGG

ITS4 (Reverse)

TCCTCCGCTTATTGATATGC

40 cycles 95°C 30 s/52°C 1 min/72°C 1 min

SNPs asociados a Cáncer

http://snpedia.com/index.php/Breast_cancer

SNPs importantes

http://www.eupedia.com/genetics/cancer_related_snp.shtml

Sitios SNPS

http://egp.gs.washington.edu/

SNPs con Genes

http://egp.gs.washington.edu/finished_genes.html

Ejemplo Click en Mapping Data

Plant rbcL gene

• rbcLa f 5’- ATGTCACCACAAACAGAGACTAAAGC-3’ (forward primer)
• rbcLa rev 5’- GTAAAATCAAGTCCACCRCG-3’ (reverse primer)

Vertebrate (non-fish) COI gene

• VF1_t1 5'-TCTCAACCAACCACAAAGACATTGG-3' (forward primer)
• VR1d_t1 5'-TAGACTTCTGGGTGGCCRAARAAYCA-3' (reverse primer)

Fish COI gene

• VF2_t1 5'-CAACCAACCACAAAGACATTGGCAC-3' (forward primer)
• FishR2_t15'-ACTTCAGGGTGACCGAAGAATCAGAA-3' (reverse primer )

Fungi ITS

• ITS1 F 5’-TCCGTAGGTGAACCTGCGG-3’ (forward primer)
• ITS4 R 5’-TCCTCCGCTTATTGATATGC-3’ (reverse primer)

Invertebrate COI gene

• LCO1490_F 5’-GGTCAACAAATCATAAAGATATTGG-3’ (forward primer)
• HC02198_R 5’-TAAACTTCAGGGTGACCAAAAAATCA-3’ (reverse primer)