4. Notas

“Non-hazardous, non-toxic, no chemical material. It is DNA sample for sequencing service purpose. No liquid is contained. Item is wrapped within a tube or a plate”

- Dudas, se escribe a sequencing@macrogenusa.com

- Descargar el TSA (abajo) y completarlo

- Llamar a FEDEX para coordinar entrega 800 463-3339

Lista de Primers Universales usados en Macrogen

1492R TACGGYTACCTTGTTACGACTT posición 1492-1513

27F AGAGTTTGATCMTGGCTCAG posición 8-27

Aprox 1500

11. Predicción de péptido de tránsito

https://services.healthtech.dtu.dk/service.php?DeepLoc-1.0

https://services.healthtech.dtu.dk

https://scialert.net/fulltext/?doi=pjbs.2017.306.313

ITS1 (Ribosomales para Levaduras)

TCCGTAGGTGAACCTGCGG

ITS4 (Reverse)

TCCTCCGCTTATTGATATGC

40 cycles 95°C 30 s/55°C 1 min/72°C 1.5 min

HTTPS://WWW.OMICSONLINE.ORG/A-SPECIES-SPECIFIC-PCR-BASED-ASSAY-FOR-RAPID-DETECTION-OF-MANGO-ANTHRACNOSE-PATHOGEN-COLLETOTRICHUM-GLOEOSPORIOIDES-PENZ-AND-SACC-2157-7471.1000184.PHP?AID=14947&VIEW=MOBILE

HTTP://SITES.BIOLOGY.DUKE.EDU/FUNGI/MYCOLAB/PRIMERS.HTM

White, T. J., T. Bruns, S. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications, eds. Innis, M. A., D. H. Gelfand, J. J. Sninsky, and T. J. White. Academic Press, Inc., New York..©W

Ejemplo de como se pueden usar

http://aem.asm.org/content/76/21/7171.full

Cloning and sequencing of 16S rRNA genes.Three separate reactions for the bacterial 16S rRNA gene using the universal primers 27f and 1492r were run for each sample to minimize PCR bias in subsequent cloning steps, and all PCR products of water samples from the same point were further pooled together. The amplification products were purified with a QIAquick PCR cleanup kit (Qiagen, Inc., Chatsworth, CA) and cloned into the TOPO TA cloning vector pCR2.1, with TOP10 Escherichia coli transformants further selected according to the manufacturer's instructions (Invitrogen). Cloned inserts were amplified from lysed colonies by PCR with plasmid vector-specific primers M13F and M13R under the same conditions as for the 16S rRNA gene listed above. Positive clones were sequenced with an ABI 3730 automated sequencer (Invitrogen, Shanghai, China).

TABLE 2.

Oligonucleotide primers and probes used in this study

11. QUE ES UN MICROSATELITE

12. Comprar pépticos de entrada a célula

Cell Penetrating Peptide: TAT sequence for cell penetration.

13. ARNi

http://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-017-0328-7

Clay RNAi

http://www.scidev.net/asia-pacific/agriculture/news/rna-clay-green-alternative-plant-pesticides.html

Video

http://www.nature.com/nrg/multimedia/rnai/animation/index.html

https://www.nature.com/nrg/posters/rnai/nrg_rnai_poster.pdf

14. Purificación

Ni-NTA agarose

https://classes.soe.ucsc.edu/bme220l/Spring11/Reading/ExampleIMACprotocol.pdf

15. Non coding RNA

http://www.the-scientist.com/?articles.view/articleNo/48691/title/Study--Most-Long-Noncoding-RNAs-Likely-Functional/

16. Secuencias interesantes

Receptor TrpV1 chile picante

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326624/

17. Learn Pyton

Learn Python

18. Ori

ori="ATCAATGATCAACGTAAGCTTCTAAGCATGATCAAGGTGCTCACACAGTTTATCCACAACCTGAGTGGATGACATCAAGATAGGTCGTTGTATCTCCTTCCTCTCGTACTCTCATGACCACGGAAAGATGATCAAGAGAGGATGATTTCTTGGCCATATCGCAATGAATACTTGTGACTTGTGCTTCCAATTGACATCTTCAGCGCCATATTGCGCTGGCCAAGGTGACGGAGCGGGATTACGAAAGCATGATCATGGCTGTTGTTCTGTTTATCTTGTTTTGACTGAGACTTGTTAGGATAGACGGTTTTTCATCACTGACTAGCCAAAGCCTTACTCTGCCTGACATCGACCGTAAATTGATAATGAATTTACATGCTTCCGCGACGATTTACCTCTTGATCATCGATCCGATTGAAGATCTTCAATTGTTAATTCTCTTGCCTCGACTCATAGCCATGATGAGCTCTTGATCATGTTTCCTTAACCCTCTATTTTTTACGGAAGAATGATCAAGCTGCTGCTCTTGATCATCGTTTC"

# Then, print the length of ori

print (len(ori))

Córralo en https://repl.it/repls/RemarkableExpensiveClumber

19. Curso Optogenics

https://www.embl.de/training/e-learning/optogenetics/index.html?_ga=2.38179491.1696672009.1518450284-2042839292.1467418975

20. Sitios de proteínas

https://web.expasy.org/spotlight/snapshots/

21. Inactivación del X

https://youtu.be/_pviVv7rR3g

22. Canal MacGrawhill

https://www.youtube.com/channel/UCxUHVv2k31uTOiCm4njuRfQ

23. Tutoriales