SDCA Journal of Medical Technology

Abstract

This study aimed to isolate and identify the bacteria in jeepney hand railings in Bacoor, Cavite. During the isolation and identification of bacteria, the researchers found out that almost all of the samples collected of the jeepney hand railings were positive for the presence of different microorganisms. Based on the data obtained from the experiment, the researchers have confirmed the presence of Staphyloccocus spp., Acinetobacter baumannii, Streptococcus spp., Bacillus spp., Escherichia coli, and Serratia spp. in the isolation of bacteria in the jeepney hand railings. Blood Agar Plate (BAP), MacConkey Agar (MAC), and Chocolate Agar Plate (CAP) were used as the primary media and biochemical tests for further testing. The data that gathered during experimentation and testing were analyzed using one-way analysis of variance (ANOVA) that were used to compare the mean values obtained. The researchers confirmed bacterial count differs according to tests used which is the colony forming unit; it also appeared that BAP and CAP shows the greatest number of bacteria in the evening. The swab samples from the jeepney hand railings exceed the acceptable limit of CFU; therefore, the microorganisms resented on the saw samples possibly can cause health risk to humans especially to the immunocompromised who are more susceptible and exposed to acquire illnesses.

Keywords: Jeepney hand railings, swab sample, bacteria, Bacoor, Cavite.