BBCEL-152: PLANT BIOCHEMISTRY

 (Laboratory)

About the Course

Dear learners, welcome to the practical sessions of Plant Biochemistry. The lab exercises and experiments provided in this manual are based on the syllabus that you have studied in BBCET-151. The concepts that you have studied in theory course of Plant Biochemistry will be  experienced during performing these lab experiments.

This lab course is worth 2 Credits and consists of six laboratory experiments. Appendix 1 and 2 are adopted from Experimental Biochemistry: A Student Companion by Beedu Sashidhar Rao and Vijay Deshpande. I. K. International Pvt. Ltd and provided as alternate method of Urease extraction and assay. All the experiments are designed in such a way that you shall be able to experience and connect the theoretical concepts explained so far. Here also the basic concepts on which the experimental procedures are based have been discussed as required.

Expected Learning Outcomes

The broad objective of this lab course is to enable you to: 

Self Learning Material

BBCEL-152.pdf

We advise you to go through respective units of BBCET-151 before you come to attend the practical sessions. This will enable you to easily understand the purpose of doing experiments and their applications. You should also read the principles of each experiment of this course along with procedure before you start performing the experiment. It is always good to prepare all the reagents freshly and store them under prearranged storage conditions. Adhere to all the safety measures and follow the safety instructions while handling the reagents. One of the good laboratory practices is to maintain your log books up-to-date i.e., enter the observations made while performing the experiments. Carry this laboratory manual and your log book during lab sessions.

Like all other IGNOU laboratory courses, this is an intensive residential exercise requiring one week for completing 2 credits. Everyday there will be two laboratory sessions of 4 hours each. So there will be a total of 14 sessions. The first session will be introductory and the remaining 2nd to 12th sessions will be based on the exercises given in the course. A schedule for laboratory exercises will be given to you in the first session. Sessions 1 to 12 shall have guided exercises under the supervision of the Academic Counsellor. The last two sessions i.e., 13th and 14th will be unguided sessions and that shall be the term end examination. In each session you shall perform exercises for 3 hours and in the remaining 1 hour you are advised to complete your practical note book. The laboratory notebook must be submitted to the counsellor for corrections and grading. 70% marks have been allocated for doing the experiments and for recording it properly. You are aware that there is a time constraint as you will have limited access to laboratory work; therefore, you are required not to miss any of the laboratory sessions.