Welcome to the laboratory course on Gene expression and regulation, BBCL-124. In this laboratory course, you will perform experiments to isolate nucleic acids and their estimations, along with determining purity. This laboratory course is connected with the core course BBCCT-123. The basics of these experiments will help you for better performance in the experiments in upcoming courses.

The first experiment of this course demonstrates how to isolate total nucleic acids from plant tissue. The following experiment is on isolating nucleic acids from the onion bulb. In the third experiment, you will isolate mRNA using the TRIzol reagent. In the fourth experiment, you will quantify the RNA using the Orcinol method. The last experiment is the determination of the purity of nucleic acids by spectrophotometry. Learners are advised to follow the standard operating procedures and instructions given by the respective counsellors and instructors.