BBCCL-108

Enzymes (Practical)

About the Course

This biochemistry laboratory course is for the learners of undergraduate programme of biochemistry with emphasis on enzymes. The lab course is worth two credits. It is our belief that all students of biochemistry, regardless of their primary interest within the discipline, will profit from a working knowledge of the structure and function of enzymes. This laboratory manual on enzymes describes the experiments you will perform during this course. The lab exercises are designed with approach that also allows learner to learn and perform a series of experiments proceeding from a less complex to a more complex experimental design. Learners are introduced to common techniques, experimental theory and experimental problem solving in the framework of a biochemical laboratory in the area of enzymology.

Self Learning Material

BBCCL-108.pdf
BBCCL-108.pdf

Efforts have been made for a well directed approach in these exercises for our learners, more like a real-life laboratory situation. Many students/learners do lab course experiments without the understanding of background needed to design a simple experiment. They lack the sense of where to start. What volume of reagents should be prepared? What concentration? Should temperature and ionic strength be controlled? What about the pH of a reaction? What is an adequate control? This lack of experience in identifying the basic elements of an experimental design often results in the failure to perform the experiment successfully. We have tried to develop these lab exercises keeping in mind what learner do, learner will also have to think through on his/her own. Learner will have to adapt procedures to the particular experiment being performed or the equipment which is available for use. These lab exercises will help each learner to complete the experiment, analyze the data and write laboratory report in a fixed time period.

Each practical exercise begins with the ‘Introduction’ about the experiment in which theory and relevance have been discussed so that one can understand fully the importance of experiment beforehand. Protocols are covered in detail explaining how to take observations followed by calculations. Precautions to be taken while performing experiment, have invariably been included under each practical exercise. Attention has also been drawn to careful handling of chemicals, and the erroneous measurements creeping in methods described for different experiments.