BBCCL-102

Molecules of Life (Practical)

About the course

This laboratory course is of 2 credits. In this lab course, you will be introduced to the basic requirements of biochemistry lab like cleaning, maintenance and safety measures of the lab. Apart from these fundamentals you will also learnt about concepts of various qualitative and quantitative tests routinely performed in a biochemistry lab.

You are aware that this lab course is designed in connection with theory course Molecules of life (BBCCT-101) where we have described structure, classification and functions of biomolecules. Hence, in this lab course we have developed experiments that will give you an opportunity to understand the biochemical properties of biomolecules discussed in BBCCT-101.

Objectives

The broad objectives of this course are to enable you to:

  • identify the routinely used glass ware and apparatus;

  • impart maintenance and cleaning of biochemistry lab;

  • preparation of normal, molar and percent solutions;

  • perform titrations and plot graphs;

  • know the basic principles behind qualitative and quantitative tests;

  • distinguish different types of biomolecules in a given test solution; and

  • determine the purity of a given nucleic acid in a solution.

Self Learning Material

BBCCL-102.pdf

In this course we have included 3 exercises & 6 experiments, among which first 3 exercises are helpful in understanding the basic needs and requirements of a biochemistry lab. Whereas, the next 6 experiments based on the physical and chemical properties of the biomolecules. This course will help you in acquiring essential skills and concepts in performing qualitative and quantitative tests.

We advise you to go through respective blocks of BBCCT-101 before you come to attend the practical sessions. This will enable you to easily understand the purpose of doing experiments and their applications. You should also read the principles of each experiment of this course along with procedure before you start performing the experiment. It is always good to prepare all the reagents freshly and store them under prearranged storage conditions. Adhere to all the safety measures and follow the safety instructions while handling the reagents.

Video Resources