This laboratory course is designed in connection with the core theory course, genetic engineering and biotechnology-BBCCT-125. There is a total of five experiments; in the first experiment, you will isolate plasmid DNA from E. coli.

The second experiment is designed for the digestion of plasmid DNA using restriction endonucleases. The third and most crucial experiment regarding research applications is DNA amplification by polymerase chain reaction (PCR). In the fourth experiment, learners will demonstrate the transformation of E. coli cells with plasmid DNA. This laboratory course's final experiment is on the hyper expression of poly histidine-tagged recombinant protein and its purification.

Learners or advised to go through the principles of restriction enzymes and the polymerase chain reaction dealt with in the theory course BBCCT 125.