https://www.whitepages.com/name/Peter-D-Kwong/Washington-DC/PK3ddj5G53p

april 6 1965

Peter Kwong, Ph.D.

https://www.niaid.nih.gov/research/peter-kwong-phd-structural-biology-section

Peter Kwong, Ph.D.

Credit: NIAID

Chief, Structural Biology Section

Vaccine Research Center

Contact Information

Peter Kwong, Ph.D.

Major Areas of Research

• Structure-based vaccine design

• Neutralizing antibodies

• Atomic-level understanding of the HIV-1 envelope and its evasion from the immune system

Program Description

The Structural Biology Section (SBS) seeks to apply structural biology to the development of an effective HIV-1 vaccine. Despite the enormous potential of atomic-level design—successfully used, for example, in the development of potent drugs against the HIV-1 protease—current vaccine development makes little use of atomic-level information. We are trying to change this.

3-D X-ray crystallographic image showing the broadly neutralizing antibody b12 (green ribbon) in contact with a critical target (yellow) for vaccine developers on HIV-1 gp120 (red). Read more about this discovery.

Credit

NIAID

One area in which we and others have already made an impact is in understanding how HIV-1 is able to evade the humoral immune system. Determination of the structure of the HIV-1 gp120 envelope glycoprotein (Kwong 1998 Nature 393, 648-659), provided a physical map of the primary target of neutralizing antibodies against HIV-1 and showed how gp120 conformational diversity can prevent antibody-mediated neutralization (Kwong 2002 Nature 420, 678-682) and how N-linked carbohydrates can form an "evolving glycan shield" (Wei 2003 Nature 422, 307-312). These and other studies—including the determination of the crystal structure of the entire spike ectodomain (Pancera 2014 Nature 514, 455-461)—have led to an understanding of the molecular trickery that protects HIV-1 from the humoral immune response.

A second area in which we and others have made an impact is in understanding how human antibodies can neutralize diverse HIV-1 isolates. The VRC01 antibody uses mimicry of the CD4 receptor to neutralize over 90 percent of HIV-1 isolates, though the inability of germline versions of VRC01 to bind to HIV-1 Env and its extraordinary level of somatic hypermutation suggest roadblocks to eliciting similar antibodies (Zhou 2010 Science 329, 811-817). Antibodies that bind the V1V2-region of HIV-1 such as CAP256-VRC26, by contrast, use an anionic loop formed by recombination and neutralize at much lower levels of somatic hypermutation (Doria-Rose 2014 Nature 509, 55-62).

But can one use structural biology in vaccine design? Currently, we are following three lines of investigation.

One line involves understanding the B cell pathways that produce broadly neutralizing antibodies and seeking to replicate their development. We’ve observed select broadly neutralizing antibodies to develop similarly in multiple donors (e.g., Wu 2011 Science 333, 1593-1602; Zhou 2013 Immunity 39, 245-258; Bonsignori 2011 J. Virol. 85, 9998-10009; Doria-Rose 2014 Nature 509, 55-62) suggesting that—for select antibodies—a common set of immunogens might spur the induction and maturation of similar antibodies in the general population.

A second line involves the precise delineation of functional constraints to identify potential footholds of conservation and exposure. One functional constraint involves receptor binding—with the site on HIV-1 Env involved in binding the CD4 receptor providing a “supersite of vulnerability.” Analysis of recognition of the CD4 supersite in 14 donors suggest that steric access to the CD4 supersite is a primary physical constraint limiting antibody recognition (Zhou 2015 Cell 161, 1280-1292). Another functional constraint involves the viral fusion machinery, including the fusion peptide, which grabs hold of the target cell membrane, as an essential step in virus entry. We found the N-terminal eight residues of the fusion peptide to be a region targeted by neutralizing antibodies (Kong 2016 Science).

A third line involves the structure-based engineering of the trimeric spike ectodomain into immunogens with the ability to stimulate the induction of broadly neutralizing antibodies. The success of this line of investigation depends in part on the design of spike mimics, which are specific for broadly neutralizing antibodies and not recognized by the non- or poorly neutralizing antibodies that typically dominant the humoral immune response, and have used structure-based design to produce prefusion-stabilized HIV-1 Env spikes of appropriate antigenicity, from a variety of Env strains (Kwon 2015 NSMB 22, 522-531; Joyce 2017 Cell Rep. 21, 2992-3002; Rutten 2018 Cell Rep. 23, 584-595).

Excitingly, by combining both second and third lines of investigation, we succeeded in inducing cross-clade neutralizing antibodies in mice, guinea pigs, and rhesus macaques (Xu 2018 Nat Med. 24, 857-867). In specific, we used prefusion-stabilized Env spikes to boost fusion peptide-directed responses, induced by vaccinating with fusion peptide coupled to the highly immunogenic carrier, keyhole limpet hemocyanin (KLH), a standard workhorse of the monoclonal antibody industry. We are currently working to apply the methodologies used to discern B cell ontogenies (first line of investigation) to improve breadth, potency and consistency of responses.

Thus, by combining multiple approaches including those used in biotechnology, we have been able to elicit broadly neutralizing antibodies against HIV-1. Complete functional screening indicate resistance to these antibodies to be focused almost exclusively to the N-terminus of the fusion peptide (Dingens 2018 PLoS Pathogens 14:e1007159).

While structure-based vaccine development with HIV-1 is proceeding, we have also been working to test our structure-based approach with other viral pathogens. We have engineered a promising vaccine antigen against respiratory syncytial virus (RSV), the leading cause of hospitalization for children under five years of age. Our “conformational fixation” approach focused on a metastable neutralization-sensitive site called antigenic site Ø (zero), at the membrane-distal apex of the RSV fusion (F) glycoprotein. Immunization of mice and nonhuman primates with a site Ø-stabilized version of RSV F (called DS-Cav1) elicited antibodies many times the protective threshold (McLellan 2013 Science 342, 592-598).

We are now working to apply the insights gleaned from both RSV and HIV-1 investigations to other human pathogens.

Biography

Dr. Kwong joined the VRC as chief of the Structural Biology Section in the Laboratory of Virology in 2001. Dr. Kwong comes to the Washington area from New York City, where he conducted research in the department of biochemistry and molecular biophysics at Columbia University.

Selected Publications

Xu K, Acharya P, Kong R, Cheng C, Chuang GY, Liu K, Louder MK, O'Dell S, Rawi R, Sastry M, Shen CH, Zhang B, Zhou T, Asokan M, Bailer RT, Chambers M, Chen X, Choi CW, Dandey VP, Doria-Rose NA, Druz A, Eng ET, Farney SK, Foulds KE, Geng H, Georgiev IS, Gorman J, Hill KR, Jafari AJ, Kwon YD, Lai YT, Lemmin T, McKee K, Ohr TY, Ou L, Peng D, Rowshan AP, Sheng Z, Todd JP, Tsybovsky Y, Viox EG, Wang Y, Wei H, Yang Y, Zhou AF, Chen R, Yang L, Scorpio DG, McDermott AB, Shapiro L, Carragher B, Potter CS, Mascola JR, Kwong PD. Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1. Nat Med. 2018 Jun;24(6): 857-867.

Stewart-Jones GB, Soto C, Lemmin T, Chuang GY, Druz A, Kong R, Thomas PV, Wagh K, Zhou T, Behrens AJ, Bylund T, Choi CW, Davison JR, Georgiev IS, Joyce MG, Kwon YD, Pancera M, Taft J, Yang Y, Zhang B, Shivatare SS, Shivatare VS, Lee CC, Wu CY, Bewley CA, Burton DR, Koff WC, Connors M, Crispin M, Baxa U, Korber BT, Wong CH, Mascola JR, Kwong PD. Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G. Cell. 2016 May 5;165(4):813-26. doi: 10.1016/j.cell.2016.04.010.

Pancera M, Zhou T, Druz A, Georgiev IS, Soto C, Gorman J, Huang J, Acharya P, Chuang GY, Ofek G, Stewart-Jones GB, Stuckey J, Bailer RT, Joyce MG, Louder MK, Tumba N, Yang Y, Zhang B, Cohen MS, Haynes BF, Mascola JR, Morris L, Munro JB, Blanchard SC, Mothes W, Connors M, Kwong PD. Structure and immune recognition of trimeric pre-fusion HIV-1 Env. Nature. 2014 Oct 23;514(7523):455-61.

McLellan JS, Chen M, Joyce MG, Sastry M, Stewart-Jones GB, Yang Y, Zhang B, Chen L, Srivatsan S, Zheng A, Zhou T, Graepel KW, Kumar A, Moin S, Boyington JC, Chuang GY, Soto C, Baxa U, Bakker AQ, Spits H, Beaumont T, Zheng Z, Xia N, Ko SY, Todd JP, Rao S, Graham BS, Kwong PD. Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus. Science. 2013 Nov 1;342(6158):592-8.

Zhou T, Georgiev I, Wu X, Yang ZY, Dai K, Finzi A, Kwon YD, Scheid JF, Shi W, Xu L, Yang Y, Zhu J, Nussenzweig MC, Sodroski J, Shapiro L, Nabel GJ, Mascola JR, Kwong PD. Structural basis for broad and potent neutralization of HIV-1 by antibody VRC01. Science. 2010 Aug 13;329(5993):811-7.

Kwong PD, Wyatt R, Robinson J, Sweet RW, Sodroski J, Hendrickson WA. Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. Nature. 1998 Jun 18;393(6686):648-59.

Visit PubMed for a complete publication listing.

Structural Models

Trimeric gp120 models have been superseded by the atomic-level crystal structure of trimeric HIV-1 Env 4TVP.

Visit the RCSB PDB for a more complete listing of SBS structures.

Content last reviewed on January 7, 2020

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Form approved OMB#: 0925-0668, EXP. DATE: 04/30/2022