This project aims to complete the main projects of Blanca's.
From our meeting with Blanca on 6/28/2023:
EIB source - Will check with Jesus
Timeline & Behavior -
B: surgery @ 14mos +/- ?? Mos, recover for a week, began training after recovery, 1st: 3 days @ 30min(?) habituation (place rat on track, familiarization with rewards [sweetened condensed milk: 1:1 dilution with water] in homecage and on track), 2nd training: 2min ITI, 15sec btw sample and test, 10 trials per day or 30. 5xLeft & 5xRight randomized. 5 days a week. Criterion >=70% for 3 days in a row or 30 days max.
S: Modify protocol to do habituation to track and rewards (in homecage and at arm ends) before surgery to accelerate post surgical performance. Keep training even after reaching criterion.
General - don’t rearrange room, clean maze between animals, be sure there are distal cues (pull curtain back if in end room). Match the same training frequency. Sac rats after training completion. (A few (3-4?) were retested in the maze 1-2 weeks after completion of training to test for ‘reactivation of the memory’).
Arrays -
B: 3 in HPC (spacing?), 1 in PFC (spacing?), Coordinates?
S: 1 in HPC, 1 in PFC, fixed (no drive)
Histology -
4 slices per rat for Stephen’s (and retrospectively for Blanca’s)