Link to the current IBC newman lab protocol: https://iu.kuali.co/protocols/protocols/6388cabd555bf3003cf36d10
An offline copy of the IBC protocol can also be found on the desktop on the computer in 483.
The protocol can also be located from the biosafety website https://research.iu.edu/compliance/biosafety/index.html Additonal information on how to report spills or other compliance issues can also be found on this website.
General Practices
Hands will be washed immediately or as soon as feasible after removing gloves or other personal protective clothing as well as before exiting the laboratory.
No eating, drinking, storing food and drink for human consumption will occur in the laboratory or other work areas. This prohibition shall be well posted.
No smoking, applying cosmetics or lip balm, and/or handling of contact lenses in the laboratory or other work areas will occur.
No non-protocol associated plants will be allowed in the laboratory unless previously approved by the biosafety office. If plants are approved for lab space they cannot leave the lab and must be autoclaved.
Any potentially contaminated equipment or work surface will have a color-coded label incorporating the universal biohazard symbol including freezers, refrigerators, and incubators.
All infectious materials to be stored will be clearly labeled with the universal biohazard symbol as will the storage space.
Spills will be reported to Environmental Health and Safety Office, Biological Safety Program. If there is a spill it will be cleaned from the periphery of the spill towards the center and paper towels will be placed over the spill. All cleaning materials will be disposed of in an appropriate manner.
Specialized spill procedures are required: Evacuate immediate area for 30 minutes, post signage on all entrances and notify Environmental Health and Safety Office, Biological Safety Program
No mouth pipetting will occur. Mouth pipetting may lead to accidental ingestion of biological specimens.
All experiments will be performed in a manner to reduce aerosol generation
Long hair will be restrained so that it cannot contact hands, specimens, containers, or equipment
Non-Animal Research Personal Protective Equipment (PPE)
Closed toe shoes
Long pants or skirts that cover the legs
Gloves
Laboratory Coat
Eye Protection
Face Shield
Surgical Mask
Engineering Devices
Chemical Fume Hood (only select if required for biological work)
Health Surveillance/Immunization
Other vaccine(s)
List other vaccine(s):
tetanus
Laboratory Practices
Needles and syringes are not recapped or reused
Sharps containers are only ⅔ full before disposal
Vortexing/mixing/centrifugation performed in tightly capped tubes
Other Techniques performed on Bench Top
Describe other procedures to be performed on Bench Top
Histological processing of ABSL-1 tissues; aliquoting AAV in BSL-2 designated surgical space
Laboratory Access
Locked laboratories with limited public access
Limited to personnel listed on protocol
Limited to personnel trained for specific procedure
Lab and Surface Disinfectant
10% commercial bleach (0.5% sodium hypochlorite) with 10 minutes contact time
Equipment Disinfectant
Solid Waste
Puncture resistant container with biohazard symbol: autoclaved prior to disposal
Other
Describe other solid waste disposal:
An autoclave is not available, accordingly, we will coordinate with EHS for disposal.
Liquid Waste
Animal Research Personal Protective Equipment (PPE) and Safety Equipment
Closed toe shoes
Long pants or skirts that cover the legs
Gloves
Disposable Gown
Animal Restraint
Animal Carcass Waste Disposal
Mammalian/avian animal carcasses are frozen, EHS is contacted to pick them up and dispose of them (Bloomington)