The detailed story of my PhD thesis

The following articles were published from my thesis.

1. Brassicaceae Mustards: Traditional and Agronomic Uses in Australia and New Zealand.

Published in Molecules, Basel.

Summary: Summarizes the uses of mustard, its proteins and peptides as well as the bioactive ingredients used in Australia and New Zealand traditional and complementary medicine .

2. Identification, characterization and epitope mapping of proteins encoded by putative allergenic napin genes from Brassica rapa.

Published in Clinical and Experimental Allergy.

Summary: Characterize the major gene family encoding the major rapeseed protein-napin, provides molecular insights into the genetic architecture and details understanding of the potential allergenicity by characterizing conserved epitopes through sequence analysis.

3. In Silico, Molecular Docking and In Vitro Antimicrobial Activity of the Major Rapeseed Seed Storage Proteins.

Published in Frontiers of Pharmacology.

Summary: This study analyzes the antimicrobial properties of major rapeseed proteins using in vitro disc-diffusion, microdilution method and computational biology approaches.

4. A Single Seed Protein Extraction Protocol for Characterizing Brassica Seed Storage Proteins.

Published in Agronomy.

Summary: First report of an optimized method for protein extraction in a single seed and analysis for seed storage proteins.

5. Shotgun Proteomics of Brassica rapa Seed Proteins Identifies Vicilin as a Major Seed Storage Protein in the Mature Seed.

Published in PLos ONE.

Summary: The study first time reports the identification of major proteins in rapeseed using Shotgun proteomics. It also characterizes the gene families encoding the major proteins, identified allergenic epitopes, determined their tertiary 3D-structures and their links with allergenicity.

Details about my research at SCU researcher profile https://researchportal.scu.edu.au/esploro/profile/mahmudur_rahman/overview?institution=61SCU_INST

Altogether, my PhD study provides a strong foundation on seed protein research which can be used to exploit their use by manipulating their quality, quantity and digestibility, improving their attributes for human consumption. This will help contribute to meet the global demand for additional protein sources within the foreseeable future.

Last updated: 11 August 2022

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