I am passionate about ethical research involving animals and I have a strong animal welfare interest.

During my teaching, research and research supervision at the Department of Pharmacy, Northern University Bangladesh, I was responsible for overseeing the Pharmacology lab and the animal house where we used Swiss ablino mice (Mus musculus) models, brine shrimp (Artemia salina), earthworm (Pheretima posthuman), grain insect (Tribolium castaneum), panel of bacteria and fungus, human blood clot to evaluate bioactive components.

As a part of the Pharmacology research, Swiss ablino mice models were used to test the acute toxicity study of drugs or plant materials in oral route, antinociceptive and analgesic potential  (using  acetic acid-induced writhing,  carrageenan induced paw oedema and formalin induced paw licking tests); antidiarrhoeal effect; central nervous system depressant activity; diuretic activity following the standard methods.

All the experimental protocols were approved by the Animal Ethics Committee, Northern University Bangladesh (Ref.: NUB/Pharm/AEC-2013). 

Researches using Swiss-albino mice, Mus musculus, of either sex:

i. Rahman, M., Khatun, A., Nesa, M., Hossain, H., & Jahan, I. A. (2015). Bioactive polyphenols from the methanol extract of Cnicus arvensis (L.) Roth demonstrated antinociceptive and central nervous system depressant activities in mice. Evidence-Based Complementary and Alternative Medicine, 2015.

ii. Khatun, A., Rahman, M., Rahman, M. M., Hossain, H., Jahan, I. A., & Nesa, M. L. (2016). Antioxidant, antinociceptive and CNS activities of Viscum orientale and high sensitive quantification of bioactive polyphenols by UPLC. Frontiers in pharmacology, 7, 176.

iii. Rahman, M., Khatun, A., Uddin, S. J., & Shilpi, J. A. (2016). Comparative effect of Lannea coromandelica (Houtt.) Merr. leaves and stem barks on acetic acid induced pain model in mice and chromagenic reagents: exploring the analgesic potential and phytochemical groups. Pharmacologyonline, 1(1), 146-152.

iv. Rahman, M., Khatun, A., Islam, M. M., Akter, M. N., Chowdhury, S. A., Khan, M. A. A., ... & Rahman, A. A. (2013). Evaluation of antimicrobial, cytotoxic, thrombolytic, diuretic properties and total phenolic content of Cinnamomum tamala. International Journal of Green Pharmacy (IJGP), 7(3).

v. Khatun, A., Rahman, M., Nesa, M., Looi, C. Y., Wong, W. F., Hazni, H., ... & Shilpi, J. A. (2022). Analgesic, anti-inflammatory and NF-κB inhibitory activity of aerial parts of Cestrum diurnum. Clinical Phytoscience, 8(1), 1-9.

vi. Biswas, A. S., Siraj, M. A., Saifuzzaman, M., Rahman, M., Khatun, A., Hossain, A., ... & Yasmin, F. (2014). Bioactivity of Xanthium indicum koen.(Compositae) leaf. Pharmacologyonline, 1(1), 21-28 [antidiarrhoeal effect].

vii. Afjalus, S., Chakma, N., Rahman, M., Salahuddin, M., & Kumar, S. (2012). Assessment of analgesic, antidiarrhoeal and cytotoxic activity of ethanolic extract of the whole plant of Bacopa monnieri Linn. International Research Journal of Pharmacy, 3(10), 98-101.

Researches using thrombolytic property of bioactive compounds using human blood:

i. Khatun A, Rahman M, et al., Rahman M, et al., Preliminary study on thrombolytic property of thirty six different extracts of eight Bangladeshi medicinal plants with folkloric relevance. Oriental Pharmacy and Experimental Medicine (2016). doi:10.1007/s13596-016-0247-5.

ii. Khatun A, Chowdhury UK, Jahan A, Rahman M. Cytotoxic and thrombolytic activity of the aerial part of Cestrum diurnum L. (Solanaceae). Pharmacology Online, 2014; 1: 109-113.

iii. Khatun A, Rahman M, Jahan S. Preliminary phytochemical, cytotoxic, thrombolytic and antioxidant activities of the methanol extract of Murraya exotica Linn. leaves. Oriental Pharmacy and Experimental Medicine, 2014; 14(3): 223-229. DOI: 10.1007/s13596-014-0150-x.

iv. Rahman M, et al.,  Evaluation of antimicrobial, cytotoxic, thrombolytic, diuretic properties and total phenolic content of Cinnamomum tamala. International Journal of Green Pharmacy, 2013; 7(3): 236-243. DOI: 10.4103/0973-8258.120242.

Researches on central nervous system stimulant or depressant activities using experimental rodent models:

i. Rahman, M., Khatun, A., Nesa, M., Hossain, H., & Jahan, I. A. (2015). Bioactive polyphenols from the methanol extract of Cnicus arvensis (L.) Roth demonstrated antinociceptive and central nervous system depressant activities in mice. Evidence-Based Complementary and Alternative Medicine, 2015.

ii. Khatun, A., Rahman, M., Rahman, M. M., Hossain, H., Jahan, I. A., & Nesa, M. L. (2016). Antioxidant, antinociceptive and CNS activities of Viscum orientale and high sensitive quantification of bioactive polyphenols by UPLC. Frontiers in pharmacology, 7, 176. 

Researches using pathogenic bacterial models to test antibacterial activity of bioactive compounds:

i. Rahman M, et al., In silico, molecular docking and in vitro antimicrobial activity of the major rapeseed proteins-napin and cruciferin. Frontiers of Pharmacology, 11, Article 1340 2020. DOI:10.3389/fphar.2020.01340. Published as a part of research Topic: Natural Antimicrobial Peptides: Hope for New Antibiotic Lead Molecules.

ii. Rahman M, Khatun A, et al., Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh. Pharmacology Online, 2014; 1: 3-10.

Researches using brine shrimp Artemia salina to test cytotoxicity of bioactive compounds:

i. Rahman M, et al., Phytopharmacological evaluation of leaves of Olive-Olea europaea. Pharmacology Online, 2015; 1:45-49.

ii. Khatun A, Rahman M, et al., Cytotoxicity potentials of eleven Bangladeshi medicinal plants. The Scientific World Journal. 2014; 2014: 7 pages.

iii. Khatun A, Rahman M, et al., Bioactivity of the bark of Macaranga indica Wight Ic (Euphorbiaceae). World Journal of Pharmaceutical Research, 2014; 3(10): 172-182. 

iv. Khatun A, Chowdhury UK, Jahan A, Rahman M. Cytotoxic and thrombolytic activity of the aerial part of Cestrum diurnum L. (Solanaceae). Pharmacology Online, 2014; 1: 109-113.

v. Khatun A, Rahman M, Jahan S. Preliminary phytochemical, cytotoxic, thrombolytic and antioxidant activities of the methanol extract of Murraya exotica Linn. leaves. Oriental Pharmacy and Experimental Medicine, 2014; 14(3): 223-229. DOI: 10.1007/s13596-014-0150-x.

v. Rahman M, Khatun A, et al., Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh. Pharmacology Online, 2014; 1: 3-10.

vi. Rahman M, et al.,  Evaluation of antimicrobial, cytotoxic, thrombolytic, diuretic properties and total phenolic content of Cinnamomum tamala. International Journal of Green Pharmacy, 2013; 7(3): 236-243. DOI: 10.4103/0973-8258.120242.

Researches using earthworm Pheretima Posthuma species to test anthelmintic property of bioactive compounds:

i. Rahman M. Chemical and biological screening of Ecbolium linneanum (Family- Acanthaceae) leaves and stems extracts. Masters of Pharmacy thesis submitted to Pharmacy Discipline, Khulna University.

Researches using rice insect to test insecticidal potential of medicinal herb components:

i. Rahman M. Chemical and biological screening of Ecbolium linneanum (Family- Acanthaceae) leaves and stems extracts. Masters of Pharmacy thesis submitted to Pharmacy Discipline, Khulna University.