CRISPR-based Salmonid Conservation in the Pacific Northwest

Dr. Michael Phelps

Assistant Professor, Washington State University

Abstract

Pacific salmonids are iconic species in the Pacific Northwest and under threat from a variety of sources. The unique life history of these species create significant challenges for biologist because of the complexity of managing hundreds of unique genetic stocks. It has become increasingly apparent that the effective management of Pacific salmonids will require a detailed understanding of the biology and genetics that distinguish different population groups. New molecular tools, such as CRISPR genome editing technology, are aiding in pursuit of this knowledge, allowing for unique conservation approaches that have not previously been possible. The application of novel molecular technologies that are being developed for salmonid conservation will be discussed. This will focus on our work to better understand the run dynamics, genetic stock identification (GSI) and influence of hatcheries on wild stocks using traditional and eDNA based population profiling. Looking toward the future, I will discuss ongoing work investigating the use of genome editing technology to understand fitness traits in Pacific salmonids as well as potential applications of CRISPR-based conservation interventions.