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Data taken with the thin sample resulted in a much too high characteristic decay time of ~3.3ms, compared to the expected ~85us.
Due to time constraints for setting up the confocal detection pinhole to try a different method, we took data on a different setup on the dye Alexa 488 and a solution of Flourescent Nanospheres.
The fit to the autocorrelation functions of A488 for various dilutions are shown below.
With these fits, we test the linearity of the concentration as a function of dilution through the scaling factor of the fit to the experimental autocorrelation function. We observe a linear relationship as expected.
We measure a diffusion coefficent from the characteristic decay times of 380 (um)^2 s^-1, compared to the literature value of 414 (um)^2 s^-1.
We also take data on flourescent nanospheres, which have a known radius. The diffusion coefficient calculated from this known radius of 50nm was 4.82(um)^2 s^-1, while we experimentally measured a diffusion coefficient of 3.1 ± 0.2 (um)^2 s^-1.