Biolab 03 Enzyme Action

PART TWO Experimental Setup

This lab has you creating an experiment for your team to learn more about the catalase enzyme. We may not have used the optimum conditions in our first exploration. What conditions would affect the reaction rate? 

Complete the following in your science notebook! 

Science Question: What factor can impact the reaction rate of the catalase enzyme?

REQUIRED: 

• • hypothesis; answer to the science question

  • experimental setup

    • independent variable (test variables)

    • dependent variable (measurement in the experiment)

    • experimental conditions to be controlled/maintained

    • data collection chart (measurement per interval of time)

      • Independent Variable X axis

      • Dependent Variable Y axis

      • How many trials are needed?

      • How will you find average of the trials? 

PART Three - Conducting the Lab

Follow the same procedure as in the initial part of the lab (part 1) . 

Be sure to add your independent variable to the setup. In other words, how are you to affect the action of the enzymes? This needs to be implemented in the lab. 

Record the results in your lab notebook. This will be graded! 

Part Four - Lab Report

This is an individual task. You are going to create a lab report for this experiment. You must include all of the required parts. Be sure you read the requirements for the lab report and the grading rubric. Also, there is a sample lab report for you to reference. 

Include the following parts with labels on the document:

• Title of the Experiment or Activity

• Introduction

• Data Tables

• Data Analysis

• Conclusion

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SETUP NOTEBOOK:

Chart 1: Data Collection

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Chart 2: Calculations

Change in O2 %

Mean (average)

Median

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PROCEDURE:

• 1. Obtain and wear googles at all times.

  2. Obtain 20 mL of H2O2 and pour it into the biochamber.

  3. Obtain 5 mL of the catalase sample from the chicken liver and place it into the vial.

  4. Carefully place the vial into the biochamber without spilling the contents  (preventing the reaction to start too soon).

  5. Insert the sensor into the rubber gasket on the probe. 

  6. Indicate the initial (start) % oxygen in the vial on chart 1.

  7. Then start the reaction by mixing the contents of th vial with the H2O2  by gently shaking the biochamber. 

  8. Record the change of % oxygen every 0.5 minutes (30 seconds). 

  9. After 3 minutes stop collecting data. 

  10. Wash the biochamber. 

  11. Repeat the procedure steps 2-10 for a total of five trials.

  12. Calculate the change (difference) in oxygen gas % in the second chart. Then, calculate the mean (average) and median change in the final row2 of chart 2. 

There are only FOUR oxygen gas sensors. 

Keep them in the four stations.