Live Cell Imaging Systems
The BioStation IM Microscope (Nikon Instruments) widefield fluorescence live cell imaging microscope is a workhorse within the facility allowing for high quality phase, and fluorescence assessments of cell lines over long time courses requiring a controlled atmosphere and high optical magnifications. Often this is used to more cheaply optimise research approaches and experimental time frames prior to moving these projects onto more expensive and complex technologies such as confocal microscopy looking at a more focused time course.
Specifications
Equipped with a Nikon DS-Qi1MC (monochrome, 1280 x 1024) camera for fluorescence Imaging
Internal incubator with humidified atomosphere options and CO2 mixer and gas perfusion
Inverted microscope platform
Time lapse (live-cell)
2 channel fluorescence light collection alongside Phase
Flourscence filterset options, DAPI, FITTC, wtGFP, HcRed, TRITC and Cy5
40x 0.8 NA Plan Fluor objective effective magnifications 20x, 40x & 80x
Automated stage movement x,y & z with offset options for multipoint collections
Accepts glass/optical grade plastic bottom 35mm dish
Intensilight epi-fluorescence illuminator light source for fluorescence illumination
Optical Config
The IncuCyte Zoom (Sartorius) widefield fluorescence microscope is a workhorse within the facility allowing for high quality phase, and fluorescence assessments of cell lines over time courses under a controlled atmosphere. Often this is used to more cheaply optimise research approaches prior to moving these projects onto more expensive and complex technologies such as confocal microscopy
Specifications
Equipped with a Nikon DS-Qi1MC (monochrome, 1280 x 1024) camera for fluorescence Imaging
Internal incubator with humidified atomosphere options and CO2 mixer and gas perfusion
Inverted microscope platform
Time lapse (live-cell)
2 channel fluorescence light collection alongside Phase
Flourscence filterset options, DAPI, GFP, DsRed and Cy5
40x 0.8 NA Plan Fluor objective effective magnifications 20x, 40x & 80x
Automated stage movement x,y & z with offset options for multipoint collections
Accepts glass/optical grade plastic bottom 35mm dish
Intensilight epi-fluorescence illuminator light source for fluorescence illumination
Optical Config