Our Applications

As a heavily used core facility supporting a diverse range of biological disciplines, there are an extensive list of applications that are carried out within the facility. Although the list of applications is extensive, it is not exhaustive and support staff are continually working alongside our researchers to modify and develop new applications to meet ever changing research needs. For individuals seeking advice on novel or bespoke applications using the varied technologies housed within the facility please email the facility emails: medlfacs@leeds.ac.uk or medlconf@leeds.ac.uk


Flow Cytometry Applications


Protein Measurements

  • Immunophenotyping –rare subset identification
  • Quantification of protein expression
  • High Throughput proteome screening
  • Measurement of phosphorylated proteins
  • Co-localisation of protein markers using FRET
  • Cytokine/Cytometric Bead Arrays (Beads based ELISAs)
  • Lyoscreen
  • Quantification of mRNA using Prime Flow/SMART FLARE Beads (FISH-Fluorescence in situ Hybridisation)
  • High Throughput System for protein analysis (96 well plates)


Cell Viability/Apoptosis

  • Caspase Cleavage
  • PhiPhi Lux (Substate cleavage)
  • Mitochondrial Depolarisation
  • Annexin V/ Viability Dye
  • Tunnel


Proliferation/Cell Division

  • Cell Cycle/ Ploidy Assessment
  • CFSE/Cell Tracker Dye Assays
  • Cell enumeration using Counting Beads
  • Microparticle (MP)/exosome quantification


Cell Activation

  • Oxidative Burst Test
  • Intracellular Calcium Flux & magnesium ion flux
  • Drug Efflux - Hoechst efflux assessments (Side Population identification)
  • Cellular pH Measurements


Cell Purification

  • 6 Way Cell sorting
  • Index plate sorting options for clonogenic assays and single cell RNA assessment
  • Sorting transfected cell lines to ensure purity of populations for future work including imaging.


Imaging Applications

Fluorescence Microscopy

  • Immunophenotyping
  • Quantification of protein expression
  • Co-localisation (FRET)/co-compartmentalisation
  • FISH (DNA & RNA)
  • Cellular morphology
  • 3D Imaging (Z-Stacks)
  • Measurement of phosphorylated proteins
  • Cell division
  • Apoptosis

High Resolution Imaging-Confocal Microscopy

  • Sub-cellular feature enumeration (speckle counting)
  • Immunophenotyping
  • Quantification of protein expression
  • Co-localisation (FRET)/co-compartmentalisation
  • FISH (DNA & RNA)
  • Cellular morphology
  • 3D Imaging (Z-Stacks)
  • Measurement of phosphorylated proteins
  • Cell division
  • Apoptosis
  • Volume measurement

Polarised Light /Chromophore Microscopy

  • Differential Interference Contrast Microscopy (DIC)
  • Light Polarisation
  • Phase Contrast Microscopy
  • Darkfield
  • Brightfield
  • Colour quantification

Live Cell Imaging

  • Confluence
  • Angiogenesis
  • Cell migration/invasion
  • Proliferation
  • Apoptosis
  • 3D Imaging (Z-Stacks)
  • Wound healing (Scratch Wound assessment)
  • Cell Counting


High Content-High Throughput Imaging

  • Bioscreening of treatment arrays- biomarker expression & cell morphology
  • High content imaging