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As a heavily used core facility supporting a diverse range of biological disciplines, there are an extensive list of applications that are carried out within the facility. Although the list of applications is extensive, it is not exhaustive and support staff are continually working alongside our researchers to modify and develop new applications to meet ever changing research needs. For individuals seeking advice on novel or bespoke applications using the varied technologies housed within the facility please email the facility emails: medlfacs@leeds.ac.uk or medlconf@leeds.ac.uk
Flow Cytometry Applications
Flow Cytometry Applications
Protein Measurements
Protein Measurements
- Immunophenotyping –rare subset identification
- Quantification of protein expression
- High Throughput proteome screening
- Measurement of phosphorylated proteins
- Co-localisation of protein markers using FRET
- Cytokine/Cytometric Bead Arrays (Beads based ELISAs)
- Lyoscreen
- Quantification of mRNA using Prime Flow/SMART FLARE Beads (FISH-Fluorescence in situ Hybridisation)
- High Throughput System for protein analysis (96 well plates)
Cell Viability/Apoptosis
Cell Viability/Apoptosis
- Caspase Cleavage
- PhiPhi Lux (Substate cleavage)
- Mitochondrial Depolarisation
- Annexin V/ Viability Dye
- Tunnel
Proliferation/Cell Division
Proliferation/Cell Division
- Cell Cycle/ Ploidy Assessment
- CFSE/Cell Tracker Dye Assays
- Cell enumeration using Counting Beads
- Microparticle (MP)/exosome quantification
Cell Activation
Cell Activation
- Oxidative Burst Test
- Intracellular Calcium Flux & magnesium ion flux
- Drug Efflux - Hoechst efflux assessments (Side Population identification)
- Cellular pH Measurements
Cell Purification
Cell Purification
- 6 Way Cell sorting
- Index plate sorting options for clonogenic assays and single cell RNA assessment
- Sorting transfected cell lines to ensure purity of populations for future work including imaging.
Imaging Applications
Imaging Applications
Fluorescence Microscopy
Fluorescence Microscopy
- Immunophenotyping
- Quantification of protein expression
- Co-localisation (FRET)/co-compartmentalisation
- FISH (DNA & RNA)
- Cellular morphology
- 3D Imaging (Z-Stacks)
- Measurement of phosphorylated proteins
- Cell division
- Apoptosis
High Resolution Imaging-Confocal Microscopy
High Resolution Imaging-Confocal Microscopy
- Sub-cellular feature enumeration (speckle counting)
- Immunophenotyping
- Quantification of protein expression
- Co-localisation (FRET)/co-compartmentalisation
- FISH (DNA & RNA)
- Cellular morphology
- 3D Imaging (Z-Stacks)
- Measurement of phosphorylated proteins
- Cell division
- Apoptosis
- Volume measurement
Polarised Light /Chromophore Microscopy
Polarised Light /Chromophore Microscopy
- Differential Interference Contrast Microscopy (DIC)
- Light Polarisation
- Phase Contrast Microscopy
- Darkfield
- Brightfield
- Colour quantification
Live Cell Imaging
Live Cell Imaging
- Confluence
- Angiogenesis
- Cell migration/invasion
- Proliferation
- Apoptosis
- 3D Imaging (Z-Stacks)
- Wound healing (Scratch Wound assessment)
- Cell Counting
High Content-High Throughput Imaging
High Content-High Throughput Imaging
- Bioscreening of treatment arrays- biomarker expression & cell morphology
- High content imaging
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