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The facility is equipped with 2 flow cytometry analysers covering a wide variety of applications. Our systems have a range of capabilities including high throughput and high volume sampling and up to 21 simultaneous parameter collection. All our systems benefit from digital processing (Pulse Area, Height & Width measurements) and can be readily booked and reserved thorough the QReserve online calendar system. Offline analysis of data can be carried out on any of our 4 offline data analysis PCs free of charge. All our flow cytometer analysers are user run following specific training for the instrumentation from facility support staff. To organise training on a system please email: medlfacs@leeds.ac.uk.
The Cytoflex S is a digital 4 laser 13 colour flow cytometer analyser, offering improved small particle resolution and high speed/high volume sampling. Options for 2 SSC parameter detections from both the Violet 405nm laser and blue 488nm laser simultaneously for enhanced small particle detection for particles down to 200nm in size. PC is Windows 7 64bit with CytExpert V2.3 Software for data acquisition. This system is user run, but training is required from facility support staff prior to access being given.
System capabilities:
4 laser system (375nm 60mW, 405nm 80mW, 488nm 50mW & 640nm 50mW excitations)
13 fluorescent parameter measurement alongside Forward Scatter (FSC) & Side Scatter (SSC)
Possibility for blue and violet laser SSC measurements simulataneously for enhanced resolution of 200nm microparticles.
Low noise avalanche photodiode detectors for enhanced light detection
Volumetric cell enumeration
Up to 30,000 events/second
Adjustable flow rates, 10µl, 30µl, 60µl, Custom mode variable up to 250µl/min with 1 μL incremental adjustments
Automatic and manual tube loading modes
Non-pressurised system allowing chemical stimulation to be carried out during sampling
Accepts 1.5ml, 1ml and 5ml Tubes
High-throughput plate arm accept 96 well Flat, U and V bottom plates (with cooling capability)
Configurable optics
Auto Compensation capability in software
Histogram Overlay capability in software
Optical Config
Cytoflex LX (Beckman Coulter)
Cytoflex LX (Beckman Coulter)
The Cytoflex LX is our newest digital 6 laser 21 colour flow cytometer analyser, offering improved small particle resolution and high speed/high volume sampling. Options for 2 SSC parameter detections from both the Violet 405nm laser and blue 488nm laser simultaneously for enhanced small particle detection for particles down to 200nm in size. PC is Windows 7 64bit with CytExpert V2.3 Software for data acquisition. This system is user run, but training is required from facility support staff prior to access being given.
System capabilities:
6 laser system (355nm 20mW, 405nm 80mW, 488nm 50mW, 561nm 30mW , 640nm 50mW and 800nm 60mW excitations)
21 fluorescent parameter measurement alongside Forward Scatter (FSC) & Side Scatter (SSC)
Possibility for blue and violet laser SSC measurements simulataneously for enhanced resolution of 200nm microparticles.
Low noise avalanche photodiode detectors for enhanced light detection
Volumetric cell enumeration
Up to 30,000 events/second
Adjustable flow rates, 10µl, 30µl, 60µl, Custom mode variable up to 250µl/min with 1 μL incremental adjustments
Automatic and manual tube loading modes
Non-pressurised system allowing chemical stimulation to be carried out during sampling
Accepts 1.5ml, 1ml and 5ml Tubes
High-throughput plate arm accept 96 well Flat, U and V bottom plates (with cooling capability)
Configurable optics
Auto Compensation capability in software
Histogram Overlay capability in software
Optical Config
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