Laser Scanning Confocal Microscopes (LSM)
The facility is equipped with two confocal systems with a range of capabilities including fast laser scanning through a resonance scan head, live cell imaging, high-thoughput sampling, volume (z-stack) Imaging, TIRF FRAP and spectral deconvolution to name but a few. For more information about these functions please email the facility at medlconf@ leeds.ac.uk and we would be happy to provide more information and advice about these functions.
The A1R is our most capable system built on the Eclipse TiE Microscope (Nikon Instruments) providing stable time-lapse imaging with automatic focus correction system and a high speed motorized stage with a broad range of sample vessel options. The A1R LSM provides a hybrid scan head allowing for the collection of high quality images through its unique pinhole and galvano scan head or high acquisition rates with 512 x 512 pixel definition at speeds of up to 30 frames per second. This system is fitted with its own incubation chamber allowing for live cell confocal assessments in gas and temperature controlled environment.
System Capabilities
- Inverted microscope platform
- 4 Laser illumination sources (1 gas, 3 solid state) 405nm, 457nm, 471nm, 488nm, 514nm, 561nm, & 641nm
- Motorized stage with inserts allowing for slide , dish and plate formats
- Fluorescence Recovery After Photobleach (FRAP)
- Spectral deconvolution for spectral overlap of dyes
- Forster Resonance Energy Transfer (FRET)
- Time lapse (live-cell)
- Volume (z-stack) imaging/volume measurements
- Automated Multi-point-Sampling of plates from 6 well-384 well
- Large image stitching capabilities
- Auto-focus through Perfect Focus System or the fluorescence intensity presets
- Complex automated sample acquisition, including random generation of multi-point images to remove experimental bias alongside automated analysis through the JOBS software module
- Incubation options (Temperature & CO2 control)
- Simultaneous 4 fluorescence channel collection alongside transmitted light collection
Optical Config
The C1 is our workhorse confocal LSM based on the TE2000E inverted microscope (Nikon Instruments) providing stable time-lapse imaging with automatic focus correction system and an ORCA ER monochromatic camera for TIRF.
System Capabilities
- Inverted microscope platform
- 4 Laser illumination sources (1 gas, 3 solid state) 405nm, 457nm, 471nm, 488nm, 514, 561nm, & 641nm
- Total Internal Reflection Fluorescence Microscopy (TIRF)
- Forster Resonance Energy Transfer (FRET)
- Time lapse (live-cell)
- volume (z-stack) imaging
- Auto-focus through Perfect Focus System or the fluorescence intensity presets
- Incubation options (Temperature & CO2 control)
- Simultaneous 3 fluorescence channel collection alongside transmitted light collection
Optical Config