Research Output

Although the facility is a teaching facility we have a strong focus on basic and translational research in human disease. The facility proactively acts to support funding applications and project research outputs directly and indirectly through the services listed within these pages. In order to maintain these high standards and to ensure that the facility is constantly resourcing advanced research technologies as they become commercially available, we request that researchers using the facility help to support both the facility and the staff within by acknowledging their input. This support acts to strengthen cases for capital equipment grant awards and helps the facility to synergistically evolve it's capabilities with your own research needs.

Acknowledging the Facility & Staff

Although the Facility is a charged service in order to recover most of its operating costs, the full costs (including capital equipment purchases) are not passed on to the end user.

The Faculty of Medicine & Health look at the quantity and quality of publications from the University research and assess the research output related to work using our core facility. This is used as a metric of facility performance (alongside the number of usage hours and cost recovery) and affects the degree of long term support that the facility receives. As a result the facility requests acknowledgment in your research outputs including posters, talks and publications to help maintain its research profile.

Guidelines

Using data generated within the Facility

In a publication that includes data (main figures or supplemental) generated within the FCCF, please state:

“We acknowledge the University of Leeds FoMH Flow Cytometry & Imaging Facility for assistance with the generation of Flow Cytometry & Light Microscopy data”

Significant Input from Facility Support Staff

If a member of the Facility support team have worked closely with you on a project, you should acknowledge this. It is good practice to include their name in the acknowledgement section at least.

In situations where they have made significant intellectual input, you can offer them a co-authorship. This should be discussed at the earliest opportunity.

Research Awards

Capital Equipment Awards

(Under construction TBA)

Publications Within The Facility

Imaging 2015-2017

Abraham S, Scarcia M, Bagshaw RD, McMahon K, Grant G, Harvey T, Yeo M, Esteves FO, Thygesen HH, Jones PF, Speirs V, Hanby AM, Selby PJ, Lorger M, Dear TN, Pawson T, Marshall CJ, Mavria G (2015) A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis. Nat Commun. 6:7286. doi: 10.1038/ncomms8286. PMID: 26129894.
Abdelhamed ZA, Natarajan S, Wheway G, Inglehearn CF, Toomes C, Johnson CA, Jagger DJ (2015). The Meckel-Gruber syndrome protein TMEM67 controls basal body positioning and epithelial branching morphogenesis in mice via the non-canonical Wnt pathway. Dis. Model Mech. 8: 527-41.
Acevedo AC, Poulter JA, Alves PG, de Lima CL, Castro LC, Yamaguti PM, Paula LM, Parry DA, Logan CV, Smith CE, Johnson CA, Inglehearn CF, Mighell AJ (2015). Variability of systemic and oro-dental phenotype in two families with non-lethal Raine syndrome with FAM20C mutations. BMC Med. Genet. 16: 8.
Cockle JV, Picton S, Levesley J, Ilett E, Carcaboso AM, Short S, Steel LP, Melcher A, Lawler SE, Brüning-Richardson A (2015). Cell migration in paediatric glioma; characterisation and potential therapeutic targeting. Br J Cancer. 112(4):693-703. doi: 10.1038/bjc.2015.16. Epub 2015. PMID: 25628092.
Cockle JV, Brüning-Richardson A, Scott KJ, Thompson J, Kottke T, Morrison E, Ismail A, Carcaboso AM, Rose A, Selby P, Conner J, Picton S, Short S, Vile R, Melcher A, Ilett E (2017). Oncolytic Herpes Simplex Virus Inhibits Pediatric Brain Tumor Migration and Invasion. Mol Ther Oncolytics. 5:75-86. doi: 10.1016/j.omto.2017.04.002. PMID: 28547002.
Cheng V, Esteves F, Chakrabarty A, Cockle J, Short S, Brüning-Richardson A (2015). High-content analysis of tumour cell invasion in three-dimensional spheroid assays. Oncoscience. 2(6):596-606. PMID: 26244167
Diggle CP, Martinez-Garay I, Molnar Z, Brinkworth MH, White E, Fowler E, Hughes R, Hayward BE, Carr IM, Watson CM, Crinnion L, Asipu A, Woodman B, Coletta PL, Markham AF, Dear TN, Bonthron DT, Peckham M, Morrison EE, Sheridan E (2017). A tubulin alpha 8 mouse knockout model indicates a likely role in spermatogenesis but not in brain development. PLoS One. 12(4):e0174264.
El-Asrag ME, Sergouniotis PI, McKibbin M, Plagnol V, Sheridan E, Waseem N, Abdelhamed Z, McKeefry D, Van Schil K, Poulter JA; UK Inherited Retinal Disease Consortium, Johnson CA, Carr IM, Leroy BP, De Baere E, Inglehearn CF, Webster AR, Toomes C, Ali M (2015). Biallelic mutations in the autophagy regulator DRAM2 cause retinal dystrophy with early macular involvement. Am J Hum Genet. 96: 948-54.
Hartill VL, van de Hoek G, Patel MP, Little R, Watson CM, Berry IR, …18 others… Norris D, Mitchison HM, Giles RH, Johnson CA (2017). DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport. Hum Mol Genet. In press.
Kamal M, Holliday DL, Morrison EE, Speirs V, Toomes C, Bell SM (2017). Loss of CSMD1 expression disrupts mammary duct formation while enhancing proliferation, migration and invasion. Oncol Rep. 38(1):283-292. doi: 10.3892/or.2017.5656. Epub
Kennedy H, Haack TB, Hartill V, Mataković L, Baumgartner ER, Potter H, Mackay R, Alston CL, O'Sullivan S, McFarland R, Connolly G, Gannon C, King R, Mead S, Crozier I, Chan W, Florkowski CM, Sage M, Höfken T, Alhaddad B, Kremer LS, Kopajtich R, Feichtinger RG, Sperl W, Rodenburg RJ, Minet JC, Dobbie A, Strom TM, Meitinger T, George PM, Johnson CA, Taylor RW, Prokisch H, Doudney K, Mayr JA (2016). Sudden cardiac death due to deficiency of the mitochondrial inorganic pyrophosphatase PPA2. Am. J. Hum. Genet. 99:674-82.
Khan KN, El-Asrag ME, Ku CA, Holder GE, McKibbin M, Arno G, Poulter JA, Carss K, Bommireddy T, Bagheri S, Bakall B, Scholl HP, Raymond FL, Toomes C, Inglehearn CF, Pennesi ME, Moore AT, Michaelides M, Webster AR Ali M; for NIHR BioResource-Rare Diseases and UK Inherited Retinal Disease Consortium (2017). Specific Alleles of CLN7/MFSD8, a Protein That Localizes to Photoreceptor Synaptic Terminals, Cause a Spectrum of Nonsyndromic Retinal Dystrophy. Invest Ophthalmol Vis Sci. 58(7):2906-2914. doi: 10.1167/iovs.16-20608.
King, H. O., Brend, T., Payne, H. L., Wright, A., Ward, T. A., Patel, K., . . . Short, S. C. (2017). RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports, 8(1), 125-139. doi:10.1016/j.stemcr.2016.12.005
Kowal-Bielecka O, Fransen J, Avouac J, Becker M, Kulak A, Allanore Y, Distler O, Clements P, Cutolo M, Czirjak L, Damjanov N, Del Galdo F, Denton CP, Distler JHW, Foeldvari I, Figelstone K, Frerix M, Furst DE, Guiducci S, Hunzelmann N, Khanna D, Matucci-Cerinic M, Herrick AL, van den Hoogen F, van Laar JM, Riemekasten G, Silver R, Smith V, Sulli A, Tarner I, Tyndall A, Welling J, Wigley F, Valentini G, Walker UA, Zulian F, Müller-Ladner U; EUSTAR Coauthors (2017). Update of EULAR recommendations for the treatment of systemic sclerosis. Ann Rheum Dis. 76(8):1327-1339. doi: 10.1136/annrheumdis-2016-209909. PMID: 27941129
Lambacher NJ, Bruel A-L, van Dam TJP, Szymańska K, Slaats GG, Kuhns S, McManus GJ, Kennedy JE, Gaff K, Wu KM, van der Lee R, Burglen L, Doummar D, Rivière J-B, Faivre L, Attié-Bitach T, Saunier S, Curd A, Peckham M, Giles RH, Johnson CA, Huynen MA, Thauvin-Robinet C, Blacque OE (2016). TMEM107 recruits ciliopathy proteins to anchored periodic subdomains of the ciliary transition zone membrane and is mutated in Joubert syndrome. Nat. Cell Biol. 18:122-31.
Levesley J, Steele L, Brüning-Richardson A, Davison A, Zhou J, Ding C, Lawler S, Short SC (2017). Selective BCL-XL inhibition promotes apoptosis in combination with MLN8237 in medulloblastoma and pediatric glioblastoma cells. Neuro Oncol. doi: 10.1093/neuonc/nox134. PMID: 29016820
Logan CV* Cossins J, Rodriguez Cruz PM, Parry DA, Maxwell S, Martinez Martinez P, Riepsaame J, Abdelhamed ZA, Lake AVR, Smith M, Moran M, Robb S, Chow G, Sewry C, Hopkins PM, Sheridan E, Jayawant S, Palace J, Johnson CA+, Beeson D+ (2015). A novel congenital myasthenic syndrome caused by mutations in COL13A1, encoding the atypical non-fibrillar collagen type XIII α1 chain. Am J Hum Genet 97: 878-85.
Makrythanasis P, Kato M, Zaki MS, Saitsu H, Nakamura K, Santoni FA, Miyatake S, Nakashima M, Issa MY, Guipponi M, Letourneau A, Logan CV, Roberts N, Parry DA, Johnson CA, Matsumoto N, Hamamy H, Sheridan E, Kinoshita T, Antonarakis SE, Murakami Y (2016). Pathogenic variants in PIGG cause intellectual disability with seizures and hypotonia. Am J Hum Genet. 98:615-26.
Mankouri J, Walter C, Stewart H, Bentham M, Park WS, Heo WD, Fukuda M, Griffin S, Harris M (2016). Release of Infectious Hepatitis C Virus from Huh7 Cells Occurs via a trans-Golgi Network-to-Endosome Pathway Independent of Very-Low-Density Lipoprotein Secretion. J Virol. 90(16):7159-70. doi: 10.1128/JVI.00826-16. Print 2016 Aug 15.PMID: 27226379
Nguyen TTM, Murakami Y, Sheridan E, Ehresmann S, Rousseau J, St-Denis A, Chai G, Ajeawung NF, Fairbrother L, Reimschisel T, Bateman A, Berry-Kravis E, Xia F, Tardif J, Parry DA, Logan CV, Diggle C, Bennett CP, Hattingh L, Rosenfeld JA, Perry MS, Parker MJ, Le Deist F, Zaki MS, Ignatius E, Isohanni P, Lönnqvist T, Carroll CJ, Johnson CA, Gleeson JG, Kinoshita T, Campeau PM (2017). Mutations in GPAA1, encoding a GPI transamidase complex protein, cause developmental delay, epilepsy, cerebellar atrophy, and osteopenia. Am J Hum Genet. 101:856-865.
Parry DA, Al Tassan N, Monies D, Megarbane A, Abouelhoda M, Halees A, Johnson CA, Alkuraya FS (2016). Characterizing the morbid genome of ciliopathies. Genome Biol. 17(1):242. doi: 10.1186/s13059-016-1099-5
Parry DA, Holmes TD, Gamper N, El-Sayed W, Hettiarachchi NT, Ahmed M, Cook GP, Logan CV, Johnson CA, Joss S, Peers C, Prescott K, Savic S, Inglehearn CF, Mighell AJ (2016). A homozygous STIM1 mutation impairs store-operated calcium entry and natural killer cell effector function without clinical immunodeficiency. J Allergy Clin Immunol. 137:955-7.
Parry DA, Smith CE, El-Sayed W, Poulter JA, Shore RC, Logan CV, Mogi C, Sato K, Okajima F, Harada A, Zhang H, Koruyucu M, Seymen F, Hu JC, Simmer JP, Ahmed M, Jafri H, Johnson CA, Inglehearn CF, Mighell AJ (2016). Mutations in the pH-sensing G-protein-coupled receptor GPR68 cause amelogenesis imperfecta. Am. J. Hum. Genet. 99:984-90.
Polson S., Kuchler V.B., Abbosh C., Ross E.M., Mathew, R.K., Beard H.A., Chuntharpursat-Bon E., Williams J., Da Silva B., Shao H., Patel A., Davies A.J., Droop A., Griffiths H.B.S., Chumas P., Short S.C., Lorger M., Gestwicki J., Roberts L.D., Bon, R.S. Allison S.J., Zhu S., Markowetz F., Wurdak H (2017). The small molecule KHS101 induces bioenergetic dysfunction in glioblastoma cells through inhibition of mitochondrial HSPD1, bioRxiv 205203; doi: https://doi.org/10.1101/205203
Roosing S, Hofree M, Kim S, Scott E, Copeland B, Romani M, Silhavy JL, Rosti RO, Schroth J, Mazza T, Miccinilli E, Zaki MS, Swoboda KJ, Milisa-Drautz J, Dobyns WB, Mikati MA, İncecik F, Azam M, Borgatti R, Romaniello R, Boustany RM, Clericuzio CL, D'Arrigo S, Strømme P, Boltshauser E, Stanzial F, Mirabelli-Badenier M, Moroni I, Bertini E, Emma F, Steinlin M, Hildebrandt F, Johnson CA, Freilinger M, Vaux KK, Gabriel SB, Aza-Blanc P, Heynen-Genel S, Ideker T, Dynlacht BD, Lee JE, Valente EM, Kim J, Gleeson JG (2015). Functional genome-wide siRNA screen identifies KIAA0586 as mutated in Joubert syndrome. Elife 4: e06602.
Samson A, Scott KJ, Taggart D, West EJ, Wilson E, Nuovo GJ, Thomson S, Corns R, Mathew RK, Fuller MJ et al (2017/8). Intravenous Delivery of Oncolytic Reovirus to Brain Tumor Patients Immunologically Primes for Subsequent Checkpoint Blockade. Science Translational Medicine. In press.
Samson A, Bentham MJ, Scott K, Nuovo G, Bloy A, Appleton E, Adair RA, Dave R, Peckham-Cooper A, Toogood G, Nagamori S, Coffey M, Vile R, Harrington K, Selby P, Errington-Mais F, Melcher A, Griffin S (2016). Oncolytic reovirus as a combined antiviral and anti-tumour agent for the treatment of liver cancer. Gut. pii: gutjnl-2016-312009. doi: 10.1136/gutjnl-2016-312009. PMID: 27902444
Savic S, Parry D, Carter C, Johnson C, Logan C, Gutierrez BM, Thomas JE, Bacon CM, Cant A, Hambleton S (2015). A new case of Fas-associated death domain protein deficiency and update on treatment outcomes. J Allergy Clin Immunol. 136: 502-5.
Schmidts M, Hou Y, Cortés CR, Mans DA, Huber C, Boldt K, Patel M, van Reeuwijk J, Plaza JM, van Beersum SE, Yap ZM, Letteboer SJ, Taylor SP, Herridge W, Johnson CA, Scambler PJ, Ueffing M, Kayserili H, Krakow D, King SM; UK10K, Beales PL, Al-Gazali L, Wicking C, Cormier-Daire V, Roepman R, Mitchison HM, Witman GB (2015). TCTEX1D2 mutations underlie Jeune asphyxiating thoracic dystrophy with impaired retrograde intraflagellar transport. Nat. Commun. 6: 7074.
Shaheen R, Szymanska K, Basu B, Patel N, Ewida N, Faqeih E, Al Hashem A, Derar N, Alsharif H, Aldahmesh MA, Alazami AM, Hashem M, Ibrahim N, Abdulwahab FM, Sonbul R, Alkuraya H, Alnemer M, Al Tala S, Al-Husain M, Morsy H, Seidahmed MZ, Meriki N, Al-Owain M, AlShahwan S, Tabarki B, Salih MA; Ciliopathy WorkingGroup, Faquih T, El-Kalioby M, Ueffing M, Boldt K, Logan CV, Parry DA, Al Tassan N, Monies D, Megarbane A, Abouelhoda M, Halees A, Johnson CA, Alkuraya FS (2016). Characterizing the morbid genome of ciliopathies. Genome Biol. 17(1):242.
Slaats GG, Wheway G, Foletto V, Szymanska K, van Balkom BWM, Logister I, Den Ouden K, Keijzer-Veen MG, Lilien MR, Knoers NV, Johnson CA, Giles RH (2015). Screen-based identification and validation of four novel ion channels as regulators of renal ciliogenesis. J. Cell Sci. 128: 4550-9
Smith CE, Poulter JA, Levin AV, Capasso JE, Price S, Ben-Yosef T, Sharony R, Newman WG, Shore RC, Brookes SJ, Mighell AJ, Inglehearn CF (2016). Spectrum of PEX1 and PEX6 variants in Heimler syndrome. Eur J Hum Genet. 24(11):1565-1571. doi: 10.1038/ejhg.2016.62. Epub 2016 Jun 15.
Stewart H, Bartlett C, Ross-Thriepland D, Shaw J, Griffin S, Harris M (2015). A novel method for the measurement of hepatitis C virus infectious titres using the IncuCyte ZOOM and its application to antiviral screening. J Virol Methods. 218:59-65. doi: 10.1016/j.jviromet.2015.03.009. Epub 2015 Mar 20. PMID: 25796989
Szymanska K, Toedt G, Lake AVR, Wheway G, Bond J, Gibson TJ, Johnson CA (2017). High-throughput siRNA-based screening methods to identify novel ciliogenesis-associated proteins. Meth. Mol. Biol. in press.
Tardif J, Parry DA, Logan CV, Diggle C, Bennett CP, Hattingh L, Rosenfeld JA, Perry MS, Parker MJ, Le Deist F, Zaki MS, Ignatius E, Isohanni P, Lönnqvist T, Carroll CJ, Johnson CA, Gleeson JG, Kinoshita T, Campeau PM (2017). Mutations in GPAA1, encoding a GPI transamidase complex protein, cause developmental delay, epilepsy, cerebellar atrophy, and osteopenia. Am J Hum Genet. 101:856-865.
Tiernan JP, Ingram N, Marston G, Perry SL, Rushworth JV, Coletta PL, Millner PA, Jayne DG, Hughes TA (2015) CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models. Nanomedicine 10, 1223-31
Waters AM, Asfahani R, Carroll P, Bicknell L, Lescai F, Bright A, Chanudet E, Brooks A, Christou-Savina S, Osman G, Walsh P, Bacchelli C, Chapgier A, Vernay B, Bader DM, Deshpande C, O' Sullivan M, Ocaka L, Stanescu H, Stewart HS, Hildebrandt F, Otto E, Johnson CA, Szymanska K, Katsanis N, Davis E, Kleta R, Hubank M, Doxsey S, Jackson A, Stupka E, Winey M, Beales PL. (2015). The kinetochore protein, CENPF, is mutated in human ciliopathy and microcephaly phenotypes. J. Med. Genet. 52: 147-56.
Wheway G*, Schmidts M*, Mans DA*, Szymanska K*, Nguyen T-MT*, ...69 others... Doherty D+, Mitchison HM+, Roepman R+, Johnson CA+ (2015). An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nat. Cell. Biol. 17: 1074-87

Flow Cytometry 2015-2017

Alase AA, El-Sherbiny YM, Vital EM, Tobin DJ, Turner NA, Wittmann M. IFNλ Stimulates MxA Production in Human Dermal Fibroblasts via a MAPK-Dependent STAT1-Independent Mechanism. J Invest Dermatol. 2015 Dec;135(12):2935-2943. doi: 10.1038/jid.2015.317. Epub 2015 Aug 19. PMID: 26288353
Alkharobi H, Beattie J, Meade J, Devine D, El-Gendy R. Dental Pulp Cells Isolated from Teeth with Superficial Caries Retain an Inflammatory Phenotype and Display an Enhanced Matrix Mineralization Potential. Front Physiol. 2017 Apr 28;8:244. doi: 10.3389/fphys.2017.00244. eCollection 2017. PMID: 28503150
Arumugakani G, Stephenson SJ, Newton DJ, Rawstron A, Emery P, Doody GM, McGonagle D, Tooze RM. Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition. J Immunol. 2017 Jun 15;198(12):4618-4628. doi: 10.4049/jimmunol.1501761. Epub 2017 May 10. PMID: 28490574
Campbell TM, Churchman SM, Gomez A, McGonagle D, Conaghan PG, Ponchel F, Jones E. Mesenchymal Stem Cell Alterations in Bone Marrow Lesions in Patients With Hip Osteoarthritis. Arthritis Rheumatol. 2016 Jul;68(7):1648-59. doi: 10.1002/art.39622. PMID: 26866940
Care MA, Stephenson SJ, Barnes NA, Fan I, Zougman A, El-Sherbiny YM, Vital EM, Westhead DR, Tooze RM, Doody GM. Network Analysis Identifies Proinflammatory Plasma Cell Polarization for Secretion of ISG15 in Human Autoimmunity. J Immunol. 2016 Aug 15;197(4):1447-59. doi: 10.4049/jimmunol.1600624. Epub 2016 Jun 29. PMID: 27357150
Churchman SM, Boxall SA, McGonagle D, Jones EA. Predicting the Remaining Lifespan and Cultivation-Related Loss of Osteogenic Capacity of Bone Marrow Multipotential Stromal Cells Applicable across a Broad Donor Age Range. Stem Cells Int. 2017;2017:6129596. doi: 10.1155/2017/6129596. Epub 2017 Feb 19. PMID: 28298930
Cockle JV, Brüning-Richardson A, Scott KJ, Thompson J, Kottke T, Morrison E, Ismail A, Carcaboso AM, Rose A, Selby P, Conner J, Picton S, Short S, Vile R, Melcher A, Ilett E. Oncolytic Herpes Simplex Virus Inhibits Pediatric Brain Tumor Migration and Invasion. Mol Ther Oncolytics. 2017 May 2;5:75-86. doi: 10.1016/j.omto.2017.04.002. eCollection 2017 Jun 16. PMID: 28547002
Cockle JV, Rajani K, Zaidi S, Kottke T, Thompson J, Diaz RM, Shim K, Peterson T, Parney IF, Short S, Selby P, Ilett E, Melcher A, Vile R. Combination viroimmunotherapy with checkpoint inhibition to treat glioma, based on location-specific tumor profiling. Neuro Oncol. 2016 Apr;18(4):518-27. doi: 10.1093/neuonc/nov173. Epub 2015 Sep 26. PMID: 26409567
Cuthbert RJ, Fragkakis EM, Dunsmuir R, Li Z, Coles M, Marzo-Ortega H, Giannoudis PV, Jones E, El-Sherbiny YM, McGonagle D. Brief Report: Group 3 Innate Lymphoid Cells in Human Enthesis. Arthritis Rheumatol. 2017 Sep;69(9):1816-1822. doi: 10.1002/art.40150. Epub 2017 Aug 8. PMID: 28511289
Cuthbert RJ, Giannoudis PV, Wang XN, Nicholson L, Pawson D, Lubenko A, Tan HB, Dickinson A, McGonagle D, Jones E. Examining the feasibility of clinical grade CD271+ enrichment of mesenchymal stromal cells for bone regeneration. PLoS One. 2015 Mar 11;10(3):e0117855. doi: 10.1371/journal.pone.0117855. eCollection 2015. PMID: 25760857
El-Jawhari JJ, Cuthbert R, McGonagle D, Jones E, Giannoudis PV. The CD45lowCD271high Cell Prevalence in Bone Marrow Samples May Provide a Useful Measurement of the Bone Marrow Quality for Cartilage and Bone Regenerative Therapy. J Bone Joint Surg Am. 2017 Aug 2;99(15):1305-1313. doi: 10.2106/JBJS.16.01138. PMID: 28763416
El-Jawhari JJ, Sanjurjo-Rodríguez C, Jones E, Giannoudis PV. Collagen-containing scaffolds enhance attachment and proliferation of non-cultured bone marrow multipotential stromal cells. J Orthop Res. 2016 Apr;34(4):597-606. doi: 10.1002/jor.23070. Epub 2015 Dec 1. PMID: 26466765
El-Sherbiny YM, Doody GM, Kelly RJ, Hill A, Hillmen P, Cook GP. Natural killer (NK) cell function in paroxysmal nocturnal hemoglobinuria: a deficiency of NK cells, but not an NK cell deficiency. Blood. 2015 Feb 19;125(8):1351-2. doi: 10.1182/blood-2014-07-591255. No abstract available. PMID: 25700426
El-Sherbiny YM, Holmes TD, Wetherill LF, Black EV, Wilson EB, Phillips SL, Scott GB, Adair RA, Dave R, Scott KJ, Morgan RS, Coffey M, Toogood GJ, Melcher AA, Cook GP. Controlled infection with a therapeutic virus defines the activation kinetics of human natural killer cells in vivo. Clin Exp Immunol. 2015 Apr;180(1):98-107. doi: 10.1111/cei.12562. PMID: 25469725
Hunt L, Hensor EM, Nam J, Burska AN, Parmar R, Emery P, Ponchel F. T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals. Ann Rheum Dis. 2016 Oct;75(10):1884-9. doi: 10.1136/annrheumdis-2015-207991. Epub 2015 Dec 1. PMID: 27613874
Ilett E, Kottke T, Thompson J, Rajani K, Zaidi S, Evgin L, Coffey M, Ralph C, Diaz R, Pandha H, Harrington K, Selby P, Bram R, Melcher A, Vile R. Prime-boost using separate oncolytic viruses in combination with checkpoint blockade improves anti-tumour therapy. Gene Ther. 2017 Jan;24(1):21-30. doi: 10.1038/gt.2016.70. Epub 2016 Oct 25. PMID: 27779616
Kim B, Stephen SL, Hanby AM, Horgan K, Perry SL, Richardson J, Roundhill EA, Valleley EM, Verghese ET, Williams BJ, Thorne JL, Hughes TA. Chemotherapy induces Notch1-dependent MRP1 up-regulation, inhibition of which sensitizes breast cancer cells to chemotherapy. BMC Cancer. 2015 Sep 11;15:634. doi: 10.1186/s12885-015-1625-y. PMID: 26362310
King HO, Brend T, Payne HL, Wright A, Ward TA, Patel K, Egnuni T, Stead LF, Patel A, Wurdak H, Short SC. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports. 2017 Jan 10;8(1):125-139. doi: 10.1016/j.stemcr.2016.12.005. PMID: 28076755
Kottke T, Evgin L, Shim KG, Rommelfanger D, Boisgerault N, Zaidi S, Diaz RM, Thompson J, Ilett E, Coffey M, Selby P, Pandha H, Harrington K, Melcher A, Vile R. Subversion of NK-cell and TNFα Immune Surveillance Drives Tumor Recurrence. Cancer Immunol Res. 2017 Nov;5(11):1029-1045. doi: 10.1158/2326-6066.CIR-17-0175. Epub 2017 Oct 15. PMID: 29038298
Kreuz S, Holmes KB, Tooze RM, Lefevre PF. Loss of PIM2 enhances the anti-proliferative effect of the pan-PIM kinase inhibitor AZD1208 in non-Hodgkin lymphomas. Mol Cancer. 2015 Dec 8;14:205. doi: 10.1186/s12943-015-0477-z. PMID: 26643319
Levesley J, Steele L, Brüning-Richardson A, Davison A, Zhou J, Ding C, Lawler S, Short SC. Selective BCL-XL inhibition promotes apoptosis in combination with MLN8237 in medulloblastoma and pediatric glioblastoma cells. Neuro Oncol. 2017 Jul 20. doi: 10.1093/neuonc/nox134. [Epub ahead of print] PMID: 29016820
Parrish C, Scott GB, Migneco G, Scott KJ, Steele LP, Ilett E, West EJ, Hall K, Selby PJ, Buchanan D, Varghese A, Cragg MS, Coffey M, Hillmen P, Melcher AA, Errington-Mais F. Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia. Leukemia. 2015 Sep;29(9):1799-810. doi: 10.1038/leu.2015.88. Epub 2015 Mar 27. PMID: 25814029
Parry DA, Holmes TD, Gamper N, El-Sayed W, Hettiarachchi NT, Ahmed M, Cook GP, Logan CV, Johnson CA, Joss S, Peers C, Prescott K, Savic S, Inglehearn CF, Mighell AJ. J Allergy Clin Immunol. 2016 Mar;137(3):955-7.e8. doi: 10.1016/j.jaci.2015.08.051. Epub 2015 Nov 10. No abstract available. PMID: 26560041
Ponchel F, Burska AN, Hensor EM, Raja R, Campbell M, Emery P, Conaghan PG. Changes in peripheral blood immune cell composition in osteoarthritis. Osteoarthritis Cartilage. 2015 Nov;23(11):1870-8. doi: 10.1016/j.joca.2015.06.018. Epub 2015 Jul 8. PMID: 26162804
Rajani K, Parrish C, Kottke T, Thompson J, Zaidi S, Ilett L, Shim KG, Diaz RM, Pandha H, Harrington K, Coffey M, Melcher A, Vile R. Combination Therapy With Reovirus and Anti-PD-1 Blockade Controls Tumor Growth Through Innate and Adaptive Immune Responses. Mol Ther. 2016 Feb;24(1):166-74. doi: 10.1038/mt.2015.156. Epub 2015 Aug 27. PMID: 26310630
Rippaus N, Taggart D, Williams J, Andreou T, Wurdak H, Wronski K, Lorger M. Metastatic site-specific polarization of macrophages in intracranial breast cancer metastases. Oncotarget. 2016 Jul 5;7(27):41473-41487. doi: 10.18632/oncotarget.9445. PMID: 27203741
Roulstone V, Khan K, Pandha HS, Rudman S, Coffey M, Gill GM, Melcher AA, Vile R, Harrington KJ, de Bono J, Spicer J. Phase I trial of cyclophosphamide as an immune modulator for optimizing oncolytic reovirus delivery to solid tumors. Clin Cancer Res. 2015 Mar 15;21(6):1305-12. doi: 10.1158/1078-0432.CCR-14-1770. Epub 2014 Nov 25. PMID: 25424857
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Flow Cytometry Historical

1. Meade JL et al (2009) Proteolytic activation of the cytotoxic phenotype during human NK cell development. Journal of Immunology 183, 803-813.

2. Holmes TD et al (2011) A human NK cell activation/inhibition threshold allows small changes in the target cell surface phenotype to dramatically alter susceptibility to NK cells. Journal of Immunology 186:1538-45

3. Scott GB et al (2010). Identification of the BCL2/Adenovirus E1B-19K protein-interacting protein 2 (BNIP-2) as a granzyme B target during human natural killer cell-mediated killing. Biochemical Journal 431, 423-4314.

4. Churchman S et al (2012) Native CD271(+) multipotential stromal cells (MSCs) have a transcript profile indicative of multiple fates with prominent osteogenic and Wnt pathway signalling activity. Arthritis and Rheumatism in press; doi: 10.1002/art.34434.

5. Jones E et al (2010) Large-scale extraction and characterization of CD271+ multipotential stromal cells from trabecular bone in health and osteoarthritis: implications for bone regeneration strategies based on uncultured or minimally cultured multipotential stromal cells. Arthritis and Rheumatism 62:1944-54.

6. Jones E et al (2010) Mesenchymal stem cells in rheumatoid synovium: enumeration and functional assessment in relation to synovial inflammation level. Annals Rheumatic Diseases 69:450-7.

7. Hoogenkamp M et al (2009) Early chromatin unfolding by RUNX1: a molecular explanation for differential requirements during specification versus maintenance of the hematopoietic gene expression program. Blood 114:299-309

8. Barnes NA et al (2012) BLIMP-1 and STAT3 counter-regulate microRNA-21 during plasma cell differentiation. Journal of Immunology in press.

9. Doody GM et al (2007) PRDM1/BLIMP-1 Modulates IFN-γ-Dependent Control of the MHC Class I Antigen-Processing and Peptide-Loading Pathway. Journal of Immunology 179:7614-23.

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