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Introduction to PCR - BTEC 24 (1 unit)
Introduction to PCR - BTEC 24 (1 unit)
An introduction to the theoretical aspects and laboratory techniques of the Polymerase Chain Reaction (PCR). Students gain practical experience performing PCR as well as experimenting with optimization of the reaction. Applications of PCR used in biotechnology industry, basic science, forensics, epidemiology, diagnostics, and determination of evolutionary relationships will be discussed.
What we do in Intro to PCR
What we do in Intro to PCR
Set-up 3 PCR reactions: genotyping, Magnesium optimization & diagnostic PCR
Run, image & analyze 3-4 agarose gels
Document & describe your PCR experiments and analysis of your results
Practice standard calculations required for PCR
One of the most ubiquitous techniques in bioscience
Applications of PCR
Applications of PCR
Genotyping, cloning, mutation detection, sequencing, microarrays, forensics, paternity testing, and evolutionary biology.
Schedule:
Schedule:
Usually offered Fall & Spring – Thursdays (9am-1pm), 6 weeks; and Saturdays (9am-3pm), 4 weeks
Check the curent schedule here.
Pre-requisites: None. A basic understanding of DNA structure and DNA replication is recommended.
Programs: BTEC 24 is an elective for the Biotechnology AS degree, required for the Biotechnology Certificate, and an optional requirement for the Stem Cell Technology.
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