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immunoassay: ELISA - BTEC 22 (1 unit)
immunoassay: ELISA - BTEC 22 (1 unit)
Learn to set up, run and analyze one of the most currently sot after lab techniques used in industry to analyze proteins – enzyme-linked immunosorbent assay (ELISA). Examples of new procedures, technical advances, past, present and future prospects in the pharmaceutical, biotechnology and clinical laboratory settings will be discussed.
Some of what we do in Immnoassay: ELISA
Some of what we do in Immnoassay: ELISA
Discuss the underlying principles of immunoassay with focus on ELISA (enzyme-linked immunosorbent assay) & its applications.
Laboratory exercises and discussions cover qualitative and quantitative data analysis
Perform and discuss direct, indirect, sandwich, and competitive ELISA methods covered
Discussion of alternative and innovative ELISA methods such as bead based ELISA, multiplex ELISA systems & EliSpot
Quantitative data analysis and protein concentration determination using standard graphs
ELISA optimization and experimental design
Cover ELISA intra-assay & inter-assay quality control methods
Schedule:
Schedule:
Offered Fall & Spring (Day & Evening Sections) – 7wk course - Tuesdays 9am-1pm and Wednesdays 6-10pm
Check the current schedule here.
Pre-requisites: None. A basic understanding of biology (DNA, replication, transcription, translation) and chemistry is needed - recommended course preparation is Bio 11 and Chem 32.
Programs: BTEC 22 is is a major elective for the Biotechnology AS degree, a requirement for the Biotechnology Certificate, and an optional requirement for the Stem Cell Technology Certificate.
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