Advanced Techniques in Molecular Biology

Welcome to the Advanced Techniques in Molecular Biology webpage. In this section, we will explore key techniques that have transformed modern biological research: Polymerase Chain Reaction (PCR), electrophoresis, western blotting, and Sanger sequencing. These methods are essential for amplifying, separating, detecting, and analyzing DNA, RNA, and proteins. PCR enables the rapid amplification of specific genetic sequences, providing a powerful tool for genetic analysis. Electrophoresis, used for both DNA and proteins, allows us to separate biomolecules based on their size and charge. Western blotting focuses on detecting specific proteins within complex mixtures, playing a crucial role in protein analysis. Lastly, Sanger sequencing, a highly accurate method for determining DNA sequences, remains widely used for identifying mutations and sequencing smaller genomes. Together, these techniques form the backbone of molecular biology research, offering insights into genetics, cellular functions, and biotechnology.