Semester 5th
(Human Genetics-NEP)
Department of Human Genetics, Sri Pratap College, Srinagar
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Advances in Molecular Genetics and Cellular Signaling
This course provides an in-depth exploration of gene expression, regulation, and cellular communication, focusing on advanced molecular genetics. Students will examine bacterial and eukaryotic gene regulation mechanisms, such as the operon model and histone modifications. The course also covers key signaling pathways, including GPCR and RTK, which are critical for understanding cellular responses.
Hands-on experience with molecular biology techniques like DNA extraction, electrophoresis, and PCR is a significant component, equipping students with practical skills for research and clinical applications.
Unit-wise Learning Modules
This course consists of three theory units and one practical unit (Course Credit: 3+1). You may access the complete course details through the link provided below.
Gene Expression & Regulation
Gene Expression & Regulation
Explores how the genetic information is transcribed and translated into functional proteins, and how these complex processes are tightly regulated to ensure proper cellular function and adaptation to environmental cues.
Cell Signaling & Mol. Genetics
Cell Signaling & Mol. Genetics
How cells communicate through signaling pathways and how genetic information governs cellular behavior, integrating molecular mechanisms with gene regulation to maintain homeostasis and respond to stimuli.
Advanced Tech. in Mol. Biology
Advanced Tech. in Mol. Biology
Introduces key laboratory methods such as PCR, Southern blotting, DNA sequencing, and electrophoresis, which are essential for analyzing, amplifying, and visualizing genetic material in research and diagnostics.
Unit 4
Unit 4
Laboratory Component
Laboratory Component
Practical laboratory exercises to reinforce theoretical concepts
1: DNA Extraction (Phenol: Chloroform Method): Isolate genomic DNA from biological samples using organic solvents to separate nucleic acids from proteins and lipids
2: Electrophoresis (Agarose Gel Electrophoresis): Separate and visualize DNA fragments based on size using an electric field through agarose gel
3: Centrifugation: Use centrifugal force to separate cellular components or nucleic acids based on their size and density
4: PCR (Principle & Working): Amplify specific DNA sequences through repeated cycles of denaturation, annealing, and extension using thermostable DNA polymerase.
Enter into the Lab Course
Semester 5-NEP Syllabus
UNIT 1: Gene Expression & Regulation (15 Hrs)
UNIT 1: Gene Expression & Regulation (15 Hrs)
Introduction to Gene Expression and Regulation
Regulation of Bacterial Gene Transcription (Operon Model: Lac Operon, Trp Operon)
Regulation of transcription in Eukaryotes (Covalent Histone Modification: Acetylation, Methylation, Phosphorylation)
Post Transcriptional Gene Regulation (RNA Interference)
UNIT 2: Cell Signaling in Molecular Genetics (15 Hrs)
UNIT 2: Cell Signaling in Molecular Genetics (15 Hrs)
Overview of Cell Signaling: Basics of cell communication: autocrine, paracrine, and endocrine signaling.
Receptor & Types (Intracellular & Cell Surface Receptor)
GPCR and G-Protein Mediated Signaling Pathway
Enzyme Linked Receptors: Receptor Tyrosine Kinase (RTK Pathway)
UNIT 3: Advanced Techniques in Molecular Biology (15 Hrs)
UNIT 3: Advanced Techniques in Molecular Biology (15 Hrs)
Polymerase Chain Reaction (PCR)
Electrophoresis
Western Blotting
DNA Sequencing (Sanger Sequencing)
Unit 4: Practical/Lab Course (1 Credit - 30 Hrs)
Unit 4: Practical/Lab Course (1 Credit - 30 Hrs)
DNA Extraction (Phenol: Chloroform Method)
Electrophoresis (Agarose Gel Electrophoresis)
Centrifugation
PCR (Principle & Working)
Suggested Reading
Suggested Reading
Cell and Molecular Biology, 9th Edition by G. Karp
Molecular Biology of the Cell by Bruce Alberts
The Cell: A Molecular Approach by Geoffrey M. Cooper
Advanced Molecular Diagnostic Techniques Principles and Applications by S. Nandi
Fundamentals and Techniques of Biophysics and Molecular Biology by Pranav Kumar
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