UST Museum is the oldest school based museum. It was established in 1869. It was initially founded as a repository for scientific collections and antiques used for education, making it a center for learning during the Spanish colonial era. Today, the museum houses a diverse collection of art, cultural artifacts, and natural history specimens that reflect the heritage of the Philippines and the long history of UST as one of Asia's oldest educational institutions.

It contains various types of collection. There are real animal that mummified, document and tools, artifacts, artwork, religion related collection, and others.

In the front part of UST, there is an arch with the form of gate. The tradition is entering and exiting the arch. Students on UST will enter when they become a new college students. Meanwhile, the exiting tradition done after they graduated from UST.