Exercise 6

In your groups fold one of the following RNAs with and without the attached constraints using the RNAfold webserver:

>Yeast-Phe-tRNA gene

GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAUAUCUGGAGGUCCUGUGUUCGAUCCACAGAAUUCGCACCA

...............xx...................x.x...............x.....................

>Yeast-Ala-tRNA gene

GGGCGUGUGGCGUAGUCGGUAGCGCGCUCCCUUGGCGUGGGAGAGUCUCCGGTUCGAUUCCGGACUCGUCCACCA

........x......x...x.....x.......x..xx...............x.....................

>Yeast-Tyr-tRNA gene

CUCUCGGUAGCCAAGUUGGUUUAAGGCGCAAGACUGUAAAUCUUGAGAUCGGGCGTUCGACUCGCCCCCGGGAGACCA

...............xxx.xxx.....x........x.x.........xx......x..x..................

>E.coli.1 6S RNA

AUUUCUCUGAGAUGUUCGCAAGCGGGCCAGUCCCCUGAGCCGAUAUUUCAUACCACAAGAAUGUGGCGCUCCGCGGUUGGUGAGCAUGCUCGGUCCGUCCGAGAAGCCUUAAAACUGCGACGACACAUUCACCUUGAACCAAGGGUUCAAGGGUUACAGCCUGCGGCGGCAUCUCGGAGAUUCC

.........................................xxxxxxxx.xxxxxxxx..........xxx.......xxx.x...x........x.x..........xxx........x..........x......xx..........x...xx.............................