Gene knockout methodInventor: Hiroki Ueda, Genshiro Sunagawa, Kenta Sumiyama. Maki Ukai, Dimitri PerrinAbstractA method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.WO EP US JP US20180020646A1 Hiroki Ueda RikenPriority 2014-12-26 • Filing 2015-12-25 • Publication 2018-01-25
Linker for unimolecular FRET biosensor based on principle of fluorescence resonance energy transferInventor: Michiyuki Matsuda, Naoki Komatsu, Kazuhiro Aoki, Yuji Kamioka, Hiroko Yukinaga, Yoshie Inaoka, Atsuro Sakurai, Etsuko Kiyokawa, Kenta SumiyamaAbstractA linker for a unimolecular FRET biosensor based on a principle of fluorescence resonance energy transfer, the linker including: a polypeptide containing 52 to 400 amino acids residues, wherein at least 45% of a total number of the amino acid residues are glycine, alanine, or both thereof, and at least 10% of the total number of the amino acid residues are alanine.WO EP US JP US9103790B2 Michiyuki Matsuda Kyoto UniversityPriority 2010-09-27 • Filing 2013-03-15 • Grant 2015-08-11 • Publication 2015-08-11
Efficient cis-element discovery method using multiple sequence comparisons based on evolutionary relationshipsInventor: Frank H. Ruddle, Kenta Sumiyama, Chang-Bae Kim AbstractA method is described for efficiently searching non-coding DNA for known control elements. Short conserved DNA sequences can be identified among 3 or more species by selecting DNA sequences from species having a total genetic distance larger than one substitution per site at a neutrally evolving region and calculating the total substitutions using pair-wise genetic distances, with the pairs for comparison selected based on phylogenetic relationships.US US6917883B1 Frank H. Ruddle Yale UniversityPriority 2002-01-15 • Filing 2003-01-15 • Grant 2005-07-12 • Publication 2005-07-12