隅山 健太
Kenta Sumiyama
所属：
名古屋大学大学院生命農学研究科 動物科学専攻 動物遺伝育種学研究室 教授
〒464-8601 名古屋市千種区不老町 国立大学法人東海国立大学機構 名古屋大学大学院生命農学研究科 動物科学専攻 動物遺伝育種学研究室
国立研究開発法人理化学研究所・生命機能科学研究センター（客員研究員）
〒565-0871 大阪府吹田市山田丘1-3 大阪大学生命システム棟3階E301
Affiliation:
Kenta Sumiyama, Ph.D.
Laboratory of Animal Genetics and Breeding
Department of Animal Sciences
Graduate School of Bioagricultural Sciences
Nagoya University
Tokai National Higher Education and Research System
Furo-cho, Chikusa, Nagoya 464-8601, Japan
https://researchmap.jp/read0199852
プロフィール：
東京大学理学部生物学科卒、同大学院理学系研究科生物科学専攻修了、博士（理学）。国立遺伝学研究所とYale大学Frank Ruddle研で博士研究員、国立遺伝学研究所助教を経て、国立研究開発法人理化学研究所 生命機能科学研究センター（BDR）高速ゲノム変異マウス作製支援研究チーム・チームリーダー（非常勤／招聘、~2023.9.30）、名古屋大学大学院生命農学研究科 動物科学専攻 動物遺伝育種学研究室 教授。
Research Topics:
1. Highly efficient gene knockout / knock-in by improving genome editing technology
2. Highly efficient transgenesis using the Tol2 transposon system
3. Understanding cis-regulatory evolutoinary mechanism and its application to genetic engineering tools
現在の研究テーマ（詳しくはここをクリック）：
１．ゲノム編集技術改良による高効率遺伝子ノックアウト・ノックイン
２．Tol2トランスポゾンを用いた高効率トランスジェネシス
３．シス制御因子駆動型の進化メカニズム研究と発生工学への応用
脊椎生物（のゲノム）が突然変異だけでより複雑な方向に進化可能なしくみについて興味を持ち研究を進めている。分子生物学、分子進化学、発生生物学、発生工学、遺伝学、ゲノム情報学などを基盤として、ゲノム編集をはじめとする発生工学技術を開発しつつ、脊椎動物の発生制御システムの進化について研究を行っている。発生調節遺伝子の遺伝子重複進化をモデル系として、ゲノムの98％を占める非コード領域に潜む膨大な制御情報がどのように進化することでボディプラン進化など新奇機能獲得に結びつくのか、またそれがどうして淘汰されず進化しうるのかを解明し、さらにその進化原理を応用して生物表現型の操作を可能にすることが研究目標。
≪ゲノム進化プロジェクト≫
ヒトに至る動物の進化の道筋では高度な生命機能が突然変異の蓄積によって実現されてきました。なぜ生物のゲノムは突然変異によって有害変異を蓄積しすぎて絶滅したりせず、進化し続けることが可能なのでしょうか？
偶然の産物である突然変異をどのように取り入れ進化するしくみがあるのか、ゲノムの進化原理の理解と応用を目指しゲノム機能発現機能の解析を行い、一方で研究ツールとしてのゲノム編集・トランスジェネシスなどの最先端マウス発生工学技術開発を進めています。さらに、こうした技術を応用して生物に新しい機能をデザインしようとしています。
隅山 健太
RIKEN BDR HP, Researchmap, Researchgate, orcid.org/0000-0001-8785-5439
（研究室の過去在籍者）
田邉 彰 研究員
浦入 裕一 JAC派遣職員
松下恵里子 JAC派遣職員
杉原有美香 JAC派遣職員
堀 奈津美 JAC派遣職員
山中香織
西山紋惠
正脇健次
長谷川小百合
中井尚美
松本直実
最近の原著論文 (Google Scholar)：
Recent publications
62. Arthur Millius, Rikuhiro G Yamada, Hiroshi Fujishima, Kazuhiko Maeda, Daron M Standley, Kenta Sumiyama, Dimitri Perrin and Hiroki R Ueda. “Circadian ribosome profiling reveals a role for the Period2 upstream open reading frame in sleep” (2023) PNAS 120 (40) e2214636120 https://doi.org/10.1073/pnas.2214636120
61. Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki, Kenta Terai, Sachiko Komazawa-Sakon, Takao Seki, Yoshifumi Yamaguchi, Tetuo Mikami, Kimi Araki, Masaki Ohmuraya, Michiyuki Matsuda, Hiroyasu Nakano. “Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis” Communications Biology (2022) 5, 1331. accepted. 25th Nov 22, Published 05 December 2022 https://doi.org/10.1038/s42003-022-04300-0
60. Daisuke Tone, Koji L. Ode, Qianhui Zhang, Hiroshi Fujishima, Rikuhiro G. Yamada, Yoshiki Nagashima, Katsuhiko Matsumoto, Zhiqing Wen, Shota Y. Yoshida, Tomoki T. Mitani, Yuki Arisato, Rei-ichiro Ohno, Maki Ukai-Tadenuma, Junko Yoshida Garçon, Mari Kaneko, Shoi Shi, Hideki Ukai, Kazunari Miyamichi, Takashi Okada, Kenta Sumiyama, Hiroshi Kiyonari, Hiroki R. Ueda. "Distinct phosphorylation states of mammalian CaMKIIβ control the induction and maintenance of sleep" PLOS Biology (2022) 20(10) e3001813 https://doi.org/10.1371/journal.pbio.3001813 Oct 4, 2022
59. Hiroshi Ichise, Shoko Tsukamoto, Tsuyoshi Hirashima, Yoshinobu Konishi, Choji Oki, Shinya Tsukiji, Satoshi Iwano, Atsushi Miyawaki, Kenta Sumiyama, Kenta Terai, Michiyuki Matsuda. "Functional visualization of NK Cell-mediated killing of metastatic single tumor cells." eLife (2022) 11:e76269 DOI: 10.7554/eLife.76269 First Published: Feb 3, 2022
58. Jiazhou He, Masamichi Yamamoto, Kenta Sumiyama, Yumi Konagaya, Kenta Terai, Michiyuki Matsuda, Shinya Sato. "Two-photon AMPK and ATP imaging reveals the bias between rods and cones in glycolysis utility" FASEB J. (2021) e21880 DOI: 10.1096/fj.202101121R First Published:27 August 2021
57. Keiji Kawatani, Toshihiko Nambara, Nobutoshi Nawa, Hidetaka Yoshimatsu, Haruna Kusakabe, Katsuya Hirata, Akira Tanave, Kenta Sumiyama, Kimihiko Banno, Hidetoshi Taniguchi, Hitomi Arahori, Keiichi Ozono and Yasuji Kitabatake. "A human isogenic iPSC-derived cell line panel identifies major regulators of aberrant astrocyte proliferation in Down syndrome" Communications Biology (2021) volume 4, Article number: 730 (2021). Published: 14 June 2021 DOI https://doi.org/10.1038/s42003-021-02242-7
56. Ayako Imanishi, Hiroshi Ichise, Chuyun Fan, Yasuaki Nakagawa, Koichiro Kuwahara, Kenta Sumiyama, Michiyuki Matsuda, Kenta Terai. "Visualization of Spatially Controlled Vasospasm by Sympathetic Nerve-Mediated ROCK Activation" The American Journal of Pathology (2021) Volume 191, Issue 1, January 2021, Pages 194-203. Available online 16 October 2020 DOI: https://doi.org/10.1016/j.ajpath.2020.09.012.
55. Tsubasa Itoh, Miki Takeuchi, Marina Sakagami, Kazuhide Asakawa, Kenta Sumiyama, Koichi Kawakami, Takashi Shimizu, Masahiko Hibi. “Gsx2 is required for specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neural progenitors in zebrafish” Development (2020) 147: dev190603 Published 9 October 2020 doi: 10.1242/dev.190603
54. Kenju Kobachi, Sota Kuno, Shinya Sato, Kenta Sumiyama, Michiyuki Matsuda, Kenta Terai. "Biliverdin reductase-A deficiency brighten and sensitize biliverdin-binding chromoproteins" Cell Structure and Function (2020) 45(2): 131-141. Released online on: 2020/06/25 DOI: https://doi.org/10.1247/csf.20010
53. Kenta Sumiyama, Akira Tanave. "The regulatory landscape of the Dlx gene system in branchial arches: shared characteristics among Dlx bigene clusters and evolution." Development, growth & differentiation (2020) 62(5): 355-362. DOI: 10.1111/dgd.12671 REVIEW.
52. Tetsuya Watabe, Kenta Terai, Kenta Sumiyama, Michiyuki Matsuda. "Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools" ACS Sensors 2020, 5 (3), 719-730. Publication Date:February 26, 2020 https://doi.org/10.1021/acssensors.9b01941
51. Katsumi Fumoto, Hisako Takigawa-Imamura, Kenta Sumiyama, Shige H. Yoshimura, Natsumi Maehara, Akira Kikuchi. "Mark1 regulates distal airspace expansion through type I pneumocyte flattening in lung development" Journal of Cell Science (2019) 2019: jcs.235556 doi: 10.1242/jcs.235556 Published 12 November 2019
50. Tomoaki Kinjo, Kenta Terai, Shoichiro Horita, Norimichi Nomura, Kenta Sumiyama, Kaori Togashi, So Iwata and Michiyuki Matsuda. "FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics" Nature Methods (2019) volume 16, pages 1029–1036. https://doi.org/10.1038/s41592-019-0541-5
49. Hideki Ukai, Kenta Sumiyama and Hiroki R Ueda. "Next-generation Human Genetics for Organism-level Systems Biology." REVIEW paper. Current Opinion in Biotechnology (2019) 58: 137-145. https://doi.org/10.1016/j.copbio.2019.03.003
48. Yoshiyasu Ono, Yasunori Mori, Yoshihiro Egashira, Kenta Sumiyama and Shigeo Takamori. "Expression of plasma membrane calcium ATPases confers Ca2+/H+ exchange in rodent synaptic vesicles" Scientific Reports (2019) volume 9, Article number: 4289 (2019) . https://doi.org/10.1038/s41598-019-40557-y
47. Kenta Sumiyama*, Naomi Matsumoto, Junko Garçon-Yoshida, Hideki Ukai, Hiroki R. Ueda, Yo Tanaka*. "Easy and efficient production of completely embryonic-stem-cell-derived mice using a micro-aggregation device." PLOS ONE (2018) 13(9): e0203056, September 19, 2018. https://doi.org/10.1371/journal.pone.0203056 *Corresponding authors
46. Yasutaka Niwa, Genki N. Kanda, Rikuhiro G. Yamada, Shoi Shi, Genshiro A. Sunagawa, Maki Ukai-Tadenuma, Hiroshi Fujishima, Naomi Matsumoto, Koh-hei Masumoto, Mamoru Nagano, Takeya Kasukawa, James Galloway, Dimitri Perrin, Yasufumi Shigeyoshi, Hideki Ukai, Hiroshi Kiyonari, Kenta Sumiyama, Hiroki R. Ueda. "Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep" Cell Reports (2018) Volume 24, Issue 9, p2231-2247, AUGUST 28, 2018. DOI:https://doi.org/10.1016/j.celrep.2018.07.082
45. Naoki Komatsu, Kenta Terai, Ayako Imanishi, Yuji Kamioka, Kenta Sumiyama, Takashi Jin, Yasushi Okada, Takeharu Nagai, Michiyuki Matsuda. "A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging" Scientific Reports (2018) volume 8, Article number: 8984 (2018) Published 12 June 2018 DOI: 10.1038/s41598-018-27174-x
44. Yu Muta, Yoshihisa Fujita, Kenta Sumiyama, Atsuro Sakurai, M Mark Taketo, Tsutomu Chiba, Hiroshi Seno, Kazuhiro Aoki, Michiyuki Matsuda, Masamichi Imajo. "Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine" Nature communications (2018) volume 9(1) Article number: 2174 (2018). Published 05 June 2018 DOI: 10.1038/s41467-018-04527-8
43. Yaxiaer Yalikun, Yusufu Aishan, Abulaiti Mosha, Kenta Sumiyama, Yo Tanaka, "Oocyte all-surfaces’ imaging method using micro-scale rotational flow." Micro & Nano Letters (2018), 13 (3):306-311, doi: 10.1049/mnl.2017.0731 Accepted on 10th November 2017
*related—<MHS2017 Best paper awards>
42. Yumi Konagaya, Kenta Terai, Yusuke Hirao, Kanako Takakura, Masamichi Imajo, Yuji Kamioka, Norio Sasaoka, Akira Kakizuka, Kenta Sumiyama, Tomoichiro Asano, Michiyuki Matsuda."A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs" Cell reports (2017) Volume 21, Issue 9, p2628–2638, 28 November 2017 DOI: 10.1016/j.celrep.2017.10.113
41. Takuya Hiratsuka, Takeshi Sano, Hisashi Kato, Naoki Komatsu, Masamichi Imajo, Yuji Kamioka, Kenta Sumiyama, Fumiaki Banno, Toshiyuki Miyata, Michiyuki Matsuda. "Live imaging of extracellular signal‐regulated kinase and protein kinase A activities during thrombus formation in mice expressing biosensors based on Förster resonance energy transfer." Journal of Thrombosis and Haemostasis (2017) 15: 1487–1499. 2017 Apr 28. doi: 10.1111/jth.13723.
40. Yuji Kamioka, Kanako Takakura, Kenta Sumiyama and Michiyuki Matsuda. "Intravital Förster resonance energy transfer imaging reveals osteopontin‐mediated polymorphonuclear leukocyte activation by tumor cell emboli" Cancer Science (2017) 108: 226-235; Accepted manuscript online: 13 DEC 2016 04:00PM EST | DOI: 10.1111/cas.13132
39. Katsumi Fumoto, Hisako Takigawa-Imamura, Kenta Sumiyama, Tomoyuki Kaneiwa, Akira Kikuch. "Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition." Development (2017) 144: 151-162; doi: 10.1242/dev.141325
38. Hidenori Nishihara, Naoki Kobayashi, Chiharu Kimura-Yoshida, Kuo Yan, Olga Bormuth, Qiong Ding, Akiko Nakanishi, Takeshi Sasaki, Mika Hirakawa, Kenta Sumiyama, Yasuhide Furuta, Victor Tarabykin, Isao Matsuo, Norihiro Okada. "Coordinately Co-opted Multiple Transposable Elements Constitute an Enhancer for wnt5a Expression in the Mammalian Secondary Palate." PLOS Genetics (2016) 12(10): e1006380. dx.doi.org/10.1371/journal.pgen.1006380
37. Fumiya Tatsuki, Genshiro A. Sunagawa, Shoi Shi, Etsuo A. Susaki, Hiroko Yukinaga, Dimitri Perrin, Kenta Sumiyama, Maki Ukai-Tadenuma, Hiroshi Fujishima, Rei-ichiro Ohno, Daisuke Tone, Koji L. Ode, Katsuhiko Matsumoto, and Hiroki R. Ueda. "Involvement of Ca2+-Dependent Hyperpolarization in Sleep Duration in Mammals" Neuron (2016) 90: 70–85. April 6, 2016 dx.doi.org/10.1016/j.neuron.2016.02.032
36. Genshiro A. Sunagawa†, Kenta Sumiyama†, Maki Ukai-Tadenuma†, Dimitri Perrin†, Hiroshi Fujishima, Hideki Ukai, Osamu Nishimura, Shoi Shi, Rei-ichiro Ohno, Ryohei Narumi, Yoshihiro Shimizu, Daisuke Tone, Koji L. Ode, Shigehiro Kuraku and Hiroki R. Ueda*, †Co-first authors. "Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene" Cell Reports (2016) 14, 1–16, January 26, 2016. dx.doi.org/10.1016/j.celrep.2015.12.052.
35. Masafumi Noguchi, Kenta Sumiyama and Mitsuru Morimoto. "Directed migration of pulmonary neuroendocrine cells toward airway branches organizes the stereotypic location of neuroepithelial bodies" Cell Reports (2015) 13, 1–8, December 29, 2015. dx.doi.org/10.1016/j.celrep.2015.11.058.
34. Akihiro Goto, Ichiro Nakahara, Takashi Yamaguchi, Yuji Kamioka, Kenta Sumiyama, Michiyuki Matsuda, Shigetada Nakanishi, and Kazuo Funabiki. "Circuit-dependent striatal PKA and ERK signaling underlies rapid behavioral shift in mating reaction of male mice" PNAS (2015) 112, 6718-6723. published ahead of print May 11, 2015, doi:10.1073/pnas.1507121112.
33. Nilmini Hettiarachchi, Kirill Kryukov, Kenta Sumiyama, and Naruya Saitou. "Lineage specific conserved noncoding sequences of plant genomes: their possible role in nucleosome positioning." Genome Biology and Evolution (2014) 6 (9), 2527-2542. doi: 10.1093/gbe/evu188
32. Rei Mizuno, Yuji Kamioka, Kenji Kabashima, Masamichi Imajo, Kenta Sumiyama, Eiji Nakasho, Takeshi Ito, Yoko Hamazaki, Yoshihisa Okuchi, Yoshiharu Sakai, Etsuko Kiyokawa, and Michiyuki Matsuda. "In vivo imaging reveals PKA regulation of ERK activity during neutrophil recruitment to inflamed intestines" The Journal of Experimental Medicine (2014) vol. 211 no. 6 p1123-1136. doi: 10.1084/jem.20132112
31. Chris T. Amemiya, Jessica Alföldi, Alison P. Lee, Shaohua Fan, Hervé Philippe, Iain MacCallum, Ingo Braasch, Tereza Manousaki, Igor Schneider, Nicolas Rohner, Chris Organ, Domitille Chalopin, Jeramiah J. Smith, Mark Robinson, Rosemary A. Dorrington, Marco Gerdol, Bronwen Aken, Maria Assunta Biscotti, Marco Barucca, Denis Baurain, Aaron M. Berlin, Gregory L. Blatch, Francesco Buonocore, Thorsten Burmester, Michael S. Campbell, Adriana Canapa, John P. Cannon, Alan Christoffels, Gianluca De Moro, Adrienne L. Edkins, Lin Fan, Anna Maria Fausto, Nathalie Feiner, Mariko Forconi, Junaid Gamieldien, Sante Gnerre, Andreas Gnirke, Jared V. Goldstone, Wilfried Haerty, Mark E. Hahn, Uljana Hesse, Steve Hoffmann, Jeremy Johnson, Sibel I. Karchner, Shigehiro Kuraku, Marcia Lara, Joshua Z. Levin, Gary W. Litman, Evan Mauceli, Tsutomu Miyake, M. Gail Mueller, David R. Nelson, Anne Nitsche, Ettore Olmo, Tatsuya Ota, Alberto Pallavicini, Sumir Panji, Barbara Picone, Chris P. Ponting, Sonja J. Prohaska, Dariusz Przybylski, Nil Ratan Saha, Vydianathan Ravi, Filipe J. Ribeiro, Tatjana Sauka-Spengler, Giuseppe Scapigliati, Stephen M. J. Searle, Ted Sharpe, Oleg Simakov, Peter F. Stadler, John J. Stegeman, Kenta Sumiyama, Diana Tabbaa, Hakim Tafer, Jason Turner-Maier, Peter van Heusden, Simon White, Louise Williams, Mark Yandell, Henner Brinkmann, Jean-Nicolas Volff, Clifford J. Tabin, Neil Shubin, Manfred Schartl, David B. Jaffe, John H. Postlethwait, Byrappa Venkatesh, Federica Di Palma, Eric S. Lander, Axel Meyer and Kerstin Lindblad-Toh.
"The African coelacanth genome provides insights into tetrapod evolution." Nature 496, 311–316 (2013) doi:10.1038/nature12027
30. Akihiro Goto, Kenta Sumiyama, Yuji Kamioka, Eiji Nakasyo, Keisuke Ito, Mitsuhiro Iwasaki, Hideki Enomoto, and Michiyuki Matsuda. "GDNF and Endothelin 3 Regulate Migration of Enteric Neural Crest-Derived Cells via Protein Kinase A and Rac1." The Journal of Neuroscience (2013) 33(11): 4901-4912; doi: 10.1523/ JNEUROSCI.4828-12.2013
29. Kenta Sumiyama*, Tsutomu Miyake, Jane Grimwood, Andrew Stuart, Mark Dickson, Jeremy Schmutz, Frank H. Ruddle, Richard M. Myers, and Chris T. Amemiya*. “Theria-specific homeodomain and cis-regulatory element evolution of the Dlx3-4 bigene cluster in 12 different mammalian species.” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. (2012) 318 (8), 639-650. Article first published online: 5 SEP 2012 | DOI: 10.1002/jez.b.22469 *Corresponding authors
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