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Factors affecting the rate of ennzyme activity
Enzyme Experiments
Key things to consider when conducting an experimental investigation into a factor affecting enzyme activity include:
- What is an appropriate range of values to select for your independent variable?
- Have you chosen a sufficient time period for the reaction to proceed?
- Have you identified, and controlled, all relevant extraneous variables?
- Can you include a negative control condition (no enzyme) to establish baseline readings?
- Can you include a positive control condition to confirm enzyme activity?
- Is it possible to treat the enzyme with the independent variable before mixing with the substrate?
- Does the data collection method allow for sufficient precision in detecting changes to levels of product / substrate?
- Have all appropriate safety precautions been taken when handling relevant substances?
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