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Monday 2/12/2024
Today was a very productive day. We came in early to warm up our agar for the plates. Then during class time, we made our dilutions, melted the agar the rest of the way, and labeled the plates. We then combined the agar with the different dilutions, plated the mixtures up, and put them in the fridge to cool.
Tuesday 2/13/2024
Today we took our plates out of the fridge and unwrapped them. We then streaked each of them with E. Coli and rewrapped them with parafilm. We then put the plates into the incubator and leave them in there for three days. We then began the sterilization process for some of our beakers.
Wednesday 2/14/2024
Today was another day of waiting for the incubator plates to grow. During this class time, we discussed another method for our analysis. In this new method, we will be pipetting 0.5ml of E. Coli into the center of the plates and recording the diameter of the bacteria circle after the growth period. We also sterilized the rest of the beakers to prepare for tomorrow.
Thursday 2/15/2024
Today we spoke with Dr. R about methods we can use to test our hypothosis. He explained to us the idea of soaking paper discs in our different hot sauces and applying them to a plain agar plate streaked with E. Coli. We went with this method and made our first trial using this new method. One trial includes one disc per plate, each using a different dilution.
Friday 2/16/2024
Today we didn't have much time due to WHS having a half day. For the first half of class, we participated in another classmate's project. After we got back from the testing we proceeded to look at the plates we had created yesterday. After looking at them we decided that one day of incubation would be sufficient which would greatly improve our efficiency in collecting data. We also realized that there was a trend of less bacteria growth near the paper slip with the hot sauce on it.
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