Publication

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2018

Maryu G*, Miura H*, Uda Y, Komatsubara AT, Matsuda M, Aoki K (Corresponding Author)(* Equal contribution)

Live-cell imaging with genetically encoded protein kinase activity reporters

Cell Structure and Function, 2018, in press

2017

Komatsubara AT, Matsuda M, Aoki K (Corresponding Author)

Single-cell quantification of the concentration and dissociation constant of endogenous proteins

bioRxiv, doi: https://doi.org/10.1101/236422

青木一洋、宇田燿一、小田茂和、「光誘導性二量体化による細胞内シグナル伝達経路の操作」、

生体の科学 増大特集細胞多様性解明に資する光技術─見て，動かす, 68 (5): 492-493, 2017

Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K (Corresponding Author)

Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling

Proc Natl. Acad. Sci. U.S.A., 114 (45) 11962-11967, 2017, doi: 10.1073/pnas.1707190114

* Some plasmids used in this study are deposited in Addgene.

基生研プレスリリース、10月31日21面の日刊工業新聞、日経バイオテク

Miura M, Matsuda M, Aoki K (Corresponding Author)

Cross-inhibition of JNK by p38 visualized by multiplexed fluorescence imaging.

bioRxiv, doi: https://doi.org/10.1101/151852

2016

Yamao M, Aoki K, Yukinawa N, Ishii S, Matsuda M, Naoki H.

Two New FRET Imaging Measures: Linearly Proportional to and Highly Contrasting the Fraction of Active Molecules.

PLoS One. 2016, 11:e0164254. doi: 10.1371/journal.pone.0164254

Kamezaki A, Sato F, Aoki K, Asakawa K, Kawakami K, Matsuzaki F, Sehara-Fujiwara A.

Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo.

Scientific Reports, 2016, 6:28873. doi: 10.1038/srep28873

Maryu G, Matsuda M, Aoki K (Corresponding Author).

Multiplexed fluorescence imaging of ERK and Akt activities and cell-cycle progression.

Cell Structure and Function, 2016, 41:81-92. doi: 10.1247/csf.16007. 2016年度日本細胞生物学会論文賞（CSF Award）受賞

Inaba K, Oda K, Aoki K, Sone K, Ikeda Y, Miyasaka A, Kashiyama T, Fukuda T, Makii C, Arimoto T, Wada-Hiraike O, Kawana K, Yano T, Osuga Y, Fujii T.

Synergistic antitumor effects of combination of PI3K/mTOR and MEK inhibition (SAR245409 and pimasertib) in mucinous ovarian carcinoma cells by fluorescence resonance energy transfer imaging.

Oncotarget, 2016, 7:29577-91. doi: 10.18632/oncotarget.8807

青木一洋、「細胞内情報伝達系の定量的なシミュレーション」、

生体の科学 増大特集 生命動態システム科学, 65 (5): 490-491, 2017

2015

Nagata Y, Kontani K, Enami T, Kataoka K, Ishii R, Totoki Y, Kataoka TR, Hirata M, Aoki K, Nakano K, Kitanaka A, Sakata-Yanagimoto M, Egami S, Shiraishi Y, Chiba K, Tanaka H, Shiozawa Y, Yoshizato T, Suzuki H, Kon A, Yoshida K, Sato Y, Sato-Otsubo A, Sanada M, Munakata W, Nakamura H, Hama N, Miyano S, Nureki O, Shibat T, Haga H, Shimoda K, Katada T, Chiba S, Watanabe T, Ogawa S.

Variegated RHOA mutations in adult T-cell leukemia/lymphoma.

Blood, 2015, 127; 596-604. doi: https://doi.org/10.1182/blood-2015-06-644948

Yamao M, Naoki H, Kunida K, Aoki K, Matsuda M, Ishii S

Distinct predictive performance of Rac1 and Cdc42 in cell migration.

Scientific Reports, 2015, 5; 17527. doi:10.1038/srep17527

Aoki K (corresponding author)

Visualization of Intracellular Signaling with Fluorescence Resonance Energy Transfer-Based Biosensors.

Protein Modifications in Pathogenic Dysregulation of Signaling, 2015, Springer, 31-41. doi: 10.1007/978-4-431-55561-2

Komatsubara AT, Matsuda M, Aoki K (corresponding author)

Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer.

Scientific Reports, 2015, 5; 13283. doi:10.1038/srep13283

Komatsu N, Fujita Y, Matsuda M, Aoki K (corresponding author)

mTORC1 upregulation via ERK-dependent gene expression change confers intrinsic resistance to MEK inhibitors in oncogeneic KRas-mutant cancer cells.

Oncogene, 2015, 34; 5607-5616. doi:10.1038/onc.2015.16

Hiratsuka T, Fujita Y, Naoki H, Aoki K, Kamioka Y, Matsuda M.

Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse epidermis.

eLife, 2015, 4 e05178. doi: http://dx.doi.org/10.7554/eLife.05178.001

2014

2014年11月26日

Sadaie W, Harada Y, Matsuda M, Aoki K (corresponding author)

Quantitative in vivo fluorescence cross-correlation analyses highlight the importance of competitive effect in the regulation of protein-protein interactions.

Molecular and Cellular Biology, 2014, 34; 3272-90. doi: 10.1128/MCB.00087-14

Fujita Y, Komatsu N, Matsuda M, Aoki K (corresponding author )

FRET-based quantitative analysis of feedforward and feedback loops in EGFR signaling and the sensitivity to molecular targeting drugs.

FEBS Journal, 2014, 281; 3177-92. doi: 10.1111/febs.12852

Miura H, Matsuda M, Aoki K (corresponding author )

Development of a FRET biosensor with high specificity for Akt.

Cell Structure and Function, 2014, 39; 9-20. doi: http://doi.org/10.1247/csf.13018. 2014年度日本細胞生物学会論文賞（CSF Award）受賞

2013

Aoki K (corresponding author), Kumagai Y, Sakurai A, Komatsu N, Fujita Y, Shionyu C, Matsuda M.

Stochastic ERK activation induced by noise and cell-cell propagation regulates cell density-dependent proliferation.

Molecular Cell, 2013, 52; 529-40

Aoki K (corresponding author), Takahashi K, Kaizu K, Matsuda M.

A Quantitative Model of ERK MAP Kinase Phosphorylation in Crowded Media.

Scientific Reports, 2013; 3:1541.

Aoki K (corresponding author), Kamioka Y, Matsuda M.

Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: Basis of biosensor construction, live imaging, and image processing.

Development, Growth, and Differentiation. 2013; 55:515-22.

2012

Kunida K, Matsuda M, Aoki K (corresponding author).

FRET imaging and statistical signalprocessing reveal positive and negative feedback loops regulating themorphology of randomly migrating HT-1080 cells.

Journal of Cell Science, 2012;125(Pt 10):2381-92.

Aoki K, Komatsu N, Hirata E, Kamioka Y, Matsuda M.

Stable expression of FRET biosensors: A new light in cancer research.

Cancer Science, 2012;103(4):614-9. doi: 10.1111/j.1349-7006.2011.02196.x.

Aoki K, Komatsu N, Goto A, Matsuda M.

Visualization of Protein Kinase Activities in Living Cells

Protein Kinase Technologies, 3-12

2011

Komatsu N, Aoki K (corresponding author) , Yamada M, Yukinaga H, Fujita Y, Kamioka Y, Matsuda M.

Development of an optimized backbone ofFRET biosensors for kinases and GTPases.

Molecular Biology of the Cell. 2011;22:4647-56.

Aoki K (corresponding author) , Yamada M, Kunida K, Yasuda S, Matsuda M.

Processive phosphorylation of ERK MAPkinase in mammalian cells.

Proc Natl. Acad. Sci. U.S.A. 2011;108:12675-80.

Parrini MC, Sadou-Dubourgnoux A, Aoki K, Kunida K, Biondini M, Hatzoglou A, Poullet P, Formstecher E, Yeaman C, Matsuda M, Rossé C, Camonis J.

SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility.

Molecular Cell, 2011;42(5):650-61.

Kiyokawa E, Aoki K, Nakamura T, Matsuda M.

Spatiotemporal regulation of small GTPases as revealed by probes based on the principle of Forster Resonance Energy Transfer (FRET): Implications for signaling and pharmacology.

Annu Rev Pharmacol Toxicol. 2011 Feb 10;51:337-58.

2010

Mitsushima M, Aoki K, Ebisuya M, Matsumura S, Yamamoto T, Matsuda M, Toyoshima F, Nishida E.

Revolving movement of a dynamic cluster of actin filaments during mitosis.

Journal of Cell Biology, 2010;191(3):453-62.

Kamioka Y, Yasuda S, Fujita Y, Aoki K, and Matsuda M.

Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK.

Journal of Biological Chemistry, 2010;285(43):33540-8.

Yoshiki S, Matsunaga-Udagawa R, Aoki K, Kamioka Y, Kiyokawa E, and Matsuda M.

Ras and Calcium Signaling Pathways Converge at Raf1 via the Shoc2 Scaffold Protein.

Molecular Biology of the Cell, 2010;21(6):1088-96.

Matsunaga-Udagawa R, Fujita Y, Yoshiki S, Terai K, Kamioka Y, Kiyokawa E, Yugi K, Aoki K (corresponding author), and Matsuda M.

The scaffold protein Shoc2/SUR-8accelerates the interaction of Ras and Raf.

Journal of Biological Chemistry, 2010;285(10):7818-26.

2009

Aoki K, and Matsuda M.

Visualization of small GTPase activity withfluorescence resonance energy transfer-based biosensors.

Nature Protocols. 2009;4(11):1623-31. Epub 2009 Oct 15.

2008

Tsukada Y, Aoki K, Nakamura T, Sakumura Y, Matsuda M, Ishii S.

Quantification of local morphodynamics and local GTPase activity by edge evolution tracking.

PLoS Computational Biology, 2008;4(11):e1000223.

Nakamura T, Aoki K, and Matsuda M.

FRET imaging and in silico simulation: analysis of the signaling network of nerve growth factor-induced neuritogenesis.

Brain Cell Biol. 2008;36(1-4):19-30

Aoki K, Kiyokawa E, Nakamura T, and Matsuda M.

Visualization of growth signal transduction cascades in living cells with genetically encoded probes based on Forster resonance energy transfer.

Philos Trans R Soc Lond B Biol Sci. 2008;363(1500):2143-51

Nishioka T, Aoki K, Hikake K, Yoshizaki H, Kiyokawa E, and Matsuda M.

Rapid Turnover Rate of Phosphoinositides at the Front of Migrating MDCK Cells.

Molecular Biology of the Cell. 2008 19:4213-4223

Itoh RE, Kiyokawa E, Aoki K, Nishioka T, Akiyama T, and Matsuda M.

Phosphorylation and activation of the Rac1 and Cdc42 GEF Asef in A431 cells stimulated by EGF.

Journal of Cell Science, 2008 Aug 15;121(Pt 16):2635-42

2007

Aoki K, Nakamura T, Inoue T, Meyer T, and Matsuda M.

An essential role for the SHIP2-dependentnegative feedback loop in neuritogenesis of NGF-stimulated PC12 cells.

Journal of Cell Biology, 2007 Jun 4;177(5):817-27

Hisata S, Sakisaka T, Baba T, Yamada T, Aoki K, Matsuda M, Takai Y.

Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth.

Journal of Cell Biology, 2007 Aug 27;178(5):843-60.

2006

Kawase K, Nakamura T, Takaya A, Aoki K, Namikawa K, Kiyama H, Inagaki S, Takemoto H, Saltiel AR, and Matsuda M.

GTP Hydrolysis of a Rho-family GTPase TC10 promotes exocytic vesicle fusion.

Developmental Cell, 2006;11(3):411-21

Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, and Matsuda M.

Dynamics of the RAS/ERK map kinase cascade as monitored by fluorescence probes.

Journal of Biological Chemistry, 2006;281(13):8917-26. Epub 2006 Jan 17.

Yoshizaki H, Aoki K, Nakamura T, and Matsuda M.

Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes.

Biochem Soc Trans. 2006;34(Pt 5):851-4

2005

Nakamura T, Aoki K, and Matsuda M.

Monitoring spatio-temporal regulation of Ras and Rho GTPases with GFP-based FRET probes.

Methods. 2005;37(2):146-53.

Nakamura T, Aoki K, and Matsuda M.

FRET imaging in nerve growth cones reveals a high level of RhoA activity within the peripheral domain.

Brain Res Mol Brain Res. 2005;139(2):277-87.

Aoki K, Nakamura T, Fujikawa K, and Matsuda M.

Local PIP3 Accumulation Recruits Vav2 andVav3 to Activate Rac1/Cdc42 and Initiate Neurite Outgrowth in Nerve GrowthFactor-stimulated PC12 Cells.

Molecular Biology of the Cell, 2005;16(5):2207-17.

2004

Aoki K, Nakamura T, and Matsuda M.

Spatio-temporal regulation of Rac1 andCdc42 activity during nerve growth factor-induced neurite outgrowth in PC12cells.

Journal of Biological Chemistry, 2004;279(1):713-9.