Bruker
I. Pepsep Column
High Performance PepSep UHPLC nanoFlow Columns
High Performance Columns
High Sensitivity -> Quantitation
User Flexibility
Swap columns in seconds
Robustness
Tested to last >1000 injections
Affordable
Target Markets
Proteomics & Pharma – higher flow rates (nano->capLC)
Application
General Proteomics, Single Cell, Cell lysate, Immunopeptidomics, homogenized Tissues, FFPE, Biological samples, peptide mapping, PTMs, Phosphoproteomics, Peptidomics, lack of sample prep
Emitters - Causes of Poor Spray
Sample Prep
No Clean-up
Debris, salt, detergents, contaminants
LC issue
No Solvent
Blockage/leak
User Error
Instrument parameters
Mishandling
II. PreOmics
Technologies for proteomic sample processing, automation and tissue homogenization/cell lysis
Proprietary PreOmics technology and design
Reagent-based kits compatible for a wide range of applications by academics and pharma / biotech companies
iST technology
iST Kits
For protein/proteomics sample preparation
Application: Bottom-up proteomics, General proteomic investigations, Not Compatible with TMT-labeling as LYSE contains primary amines
iST-NHS Kits
For compatible with chemical labeling
Applications: Bottom-up proteomics TMTor iTRAQ labeling, General proteomic investigations, Relative quantitation experiments
iST and iST-NHS Kits
Sample types: Cell, Tissue, Model organism, Ips, Samples with chemical labeling
Key point:
Fast, Robust, Easy-to-use, high-quality sample preparation
Adaptable to high-throughput and automation
Focus on comprehensive lysis and protein extraction
Focus on optimized digestion
iST-BCT Kitsquantitation
For biological fluids
Applications: Bottom-up proteomics, General proteomic investigations,
Not Compatible with TMT-labeling as BCT-LYSE primary aminesSample types: Biological fluids (plasma, serum, whole blood, CSF, saliva) Single proteins, therapeutic proteins, HCP analysis
Key point:
Fast, Robust, Easy-to-use, high-quality sample preparation
Adaptable to high-throughput and automation
Focus on high alkylation efficiency
Focus on low artificial modifications
iST-PSI Kits
For positive pressure processing
iST Trap
For immunoprecipitation preparation
Phoenix
For peptide clean-up
iST Fractionation Add-on
For peptide fractionation
iST-PreON Kits
Protein Extraction
BeatBox Tissue kit 96x and 24x
96x
96-well plate with one Gyuto bead per well
Tissue amount 1-5 mg of tissue wet weight
Cell amount up to 6x105 of mammalian cells or 1 mL of 0.6 OD600
for yeast pellet or 0.5 OD600 for bacteria pelletThe volume of lysis buffer from 50-100 mL
24x
24x 2mL tubes with one Gyuto bead per tube
Tissue amount 5-50 mg of tissue wet weight
Cell amount up to 1x107 of mammalian cells
The volume of lysis buffer from 300-1000 mL
III. IntelliSlidesTM
Automatic reading of barcode and teach marks (with specialized software)
Simplify your MALDI Imaging workflows by enabling automated setup.
IX. Matrix
Created especially for high resolution MALDI Imaging workflows
Qualified for all of Bruker’s MALDI Imaging platforms
Extra pure to remove potential sources of contamination.
Dissolves rapidly and completely; simplifies and speeds up sample preparation.
Easily removed from tissue samples for histological staining, immunohistochemistry or genetic analyses
Consume: 2,5-Dihydroxybenzoic acid (2,5-DHB)
Application: matrices for a wide variety of peptides, proteins, polymers and carbohydrates, including phosphopeptides and glycoproteins.
**200,000 samples/200mg using Anchor plate
Consume: super-DHB (SDHB)
Application: matrices for a large intact proteins and glycoproteins, and generation of in-source decay (ISD) spectra of intact proteins.
**1,000,000 samples/500mg Anchor plate
Consume: 2,5-Dihydroxyacetophenon (2,5-DHAP)
Application: proteins (mass range between 8 and 100 kDa), preventing in-source decay (ISD) fragmentation, proteomic profiling studies and glycoproteins.
**30,000 samples/200mg
Consume: 3-Hydroxypicolinic acid (3-HPA)
Application: oligonucleotides
** 5 x 10,000 samples/200mg
Consume: α-Cyano-4-hydroxycinnamic acid (HCCA or CHCA)
Application: widely used in proteomics, peptides and proteins (0.7 to 20 kDa), nucleotides.
** 1,400,000 samples/200mg Anchor plate
Consume: Sinapinic acid
Application: widely used in proteomics, peptides and large proteins (10–150 kDa) and some polar polymers, generating of in-source decay (ISD)
**10,000 samples/200mg
X. Calibration std
Oligonucleotide Calibration Standard LMW
Application:
mass range 1000 to 4000 Da
** 5x100 calibration spots
Oligonucleotide Calibration Standard (5x100 calibration spots)
Application:
mass range 3500 to 10000 Da
** 5x100 calibration spots
Peptide Calibration Standard II
Application:
mass range 700 to 3200 Da
5x250 calibration spots
Peptide Calibration Standard
Application:
mass range 1000 and 3500 Da
5x250 calibration spots
Protein Calibration Standard II
Application:
mass range 10000 to 70000 Da
5x250 calibration spots
Protein Calibration Standard I
Application:
mass range 4000 to 20000 Da
5x250 calibration spots
fleXstandard Polymers
Application:
PMMA mixture, mass range 300 to 10000 Da
15x250 calibration spots
fleXstandard Polymers
Application:
Tryptic digest, mass range 900 to 2100 Da
15x250 calibration spots