Bruker

I. Pepsep Column

High Performance PepSep UHPLC nanoFlow Columns

High Performance Columns

High Sensitivity -> Quantitation

User Flexibility

Swap columns in seconds

Robustness

Tested to last >1000 injections

Affordable

Target Markets

Proteomics & Pharma – higher flow rates (nano->capLC)

Application

General Proteomics, Single Cell, Cell lysate, Immunopeptidomics, homogenized Tissues, FFPE, Biological samples, peptide mapping, PTMs, Phosphoproteomics, Peptidomics, lack of sample prep

Emitters - Causes of Poor Spray

Sample Prep

No Clean-up
Debris, salt, detergents, contaminants

LC issue

No Solvent
Blockage/leak

User Error

Instrument parameters
Mishandling

II. PreOmics

Technologies for proteomic sample processing, automation and tissue homogenization/cell lysis

Proprietary PreOmics technology and design
Reagent-based kits compatible for a wide range of applications by academics and pharma / biotech companies

iST technology

iST Kits

For protein/proteomics sample preparation
Application: Bottom-up proteomics, General proteomic investigations, Not Compatible with TMT-labeling as LYSE contains primary amines

iST-NHS Kits

For compatible with chemical labeling
Applications: Bottom-up proteomics TMTor iTRAQ labeling, General proteomic investigations, Relative quantitation experiments

iST and iST-NHS Kits

Sample types: Cell, Tissue, Model organism, Ips, Samples with chemical labeling
Key point:
- Fast, Robust, Easy-to-use, high-quality sample preparation
- Adaptable to high-throughput and automation
- Focus on comprehensive lysis and protein extraction
- Focus on optimized digestion

iST-BCT Kitsquantitation

For biological fluids
Applications: Bottom-up proteomics, General proteomic investigations,
Not Compatible with TMT-labeling as BCT-LYSE primary amines
Sample types: Biological fluids (plasma, serum, whole blood, CSF, saliva) Single proteins, therapeutic proteins, HCP analysis
Key point:
- Fast, Robust, Easy-to-use, high-quality sample preparation
- Adaptable to high-throughput and automation
- Focus on high alkylation efficiency
- Focus on low artificial modifications

iST-PSI Kits

For positive pressure processing

iST Trap

For immunoprecipitation preparation

Phoenix

For peptide clean-up

iST Fractionation Add-on

For peptide fractionation

iST-PreON Kits

Protein Extraction

BeatBox Tissue kit 96x and 24x

96x

96-well plate with one Gyuto bead per well
Tissue amount 1-5 mg of tissue wet weight
Cell amount up to 6x105 of mammalian cells or 1 mL of 0.6 OD600
for yeast pellet or 0.5 OD600 for bacteria pellet
The volume of lysis buffer from 50-100 mL

24x

24x 2mL tubes with one Gyuto bead per tube
Tissue amount 5-50 mg of tissue wet weight
Cell amount up to 1x107 of mammalian cells
The volume of lysis buffer from 300-1000 mL

III. IntelliSlidesTM

Automatic reading of barcode and teach marks (with specialized software)
Simplify your MALDI Imaging workflows by enabling automated setup.

IX. Matrix

Created especially for high resolution MALDI Imaging workflows

Qualified for all of Bruker’s MALDI Imaging platforms
Extra pure to remove potential sources of contamination.
Dissolves rapidly and completely; simplifies and speeds up sample preparation.
Easily removed from tissue samples for histological staining, immunohistochemistry or genetic analyses

Consume: 2,5-Dihydroxybenzoic acid (2,5-DHB)

Application: matrices for a wide variety of peptides, proteins, polymers and carbohydrates, including phosphopeptides and glycoproteins.

**200,000 samples/200mg using Anchor plate

Consume: super-DHB (SDHB)

Application: matrices for a large intact proteins and glycoproteins, and generation of in-source decay (ISD) spectra of intact proteins.

**1,000,000 samples/500mg Anchor plate

Consume: 2,5-Dihydroxyacetophenon (2,5-DHAP)

Application: proteins (mass range between 8 and 100 kDa), preventing in-source decay (ISD) fragmentation, proteomic profiling studies and glycoproteins.

**30,000 samples/200mg