LB (Luria-Bertani medium)

• 0.5% Bacto-Yeast extract 5 g
• 1% Bacto-Tryptone 10 g
• 1% NaCl 10 g/ L
• 1% Agar 10 g

※Autoclave後、20 ml/plate

10x SC-His-Trp-Leu-Ura-Ade drop out mix

• Alanine 20 g
• Arginine 20 g
• Asparagine 20 g
• Asparatic acid 20 g
• Cystein 20 g
• Glutamine 20 g
• Glutamic acid 20 g
• Inositol 20 g
• Isoleucine 20 g
• Lysine 20 g
• Methionine 20 g
• para-Aminobenzoic acid 20 g
• Phenylalanine 20 g
• Piroline 20 g
• Serine 20 g
• Threonine 20 g
• Tyrosine 20 g
• Valine 20 g

※ロータリーミキサーにかけてよく混ぜる (12 h)

※4℃保存

SC-X stocks

Take 36.2 g of the 10x SC-His-Trp-Leu-Ura-Ade mix and His, Leu, Ura, Trp or Ade as appropriate.

• Adenine 2 g
• Histidine 2 g
• Leucine 4 g
• Uracil 2 g
• or Tryptophan 2 g

※使用前によく混ぜる

※4℃保存